D. Wade Abbott, Ph. D.

Image Wade Abbott
Research Scientist

Agricultural Glycomics

Functional Genomics of Carbohydrate Utilization

Intestinal Microbiomes

Carbohydrate Active Enzymes

Current research and/or projects

  1. Principal Investigator. Abbott DW, Zandberg WF, Inglis GD, TA, T, Thomas, D., Ortega Polo, R. Title: “Muco-Screen: A platform technology to evaluate commercial and agriproduct based inhibitors for mitigation of necrotic enteritis in broiler chickens.”  Agriculture and Forestry $325K, Apr 2020-Mar 2023.

  2. Co-Applicant. Beauchemin, K, McAllister, TW, Abbott, DW, Gruninger, R. “Seaweeds and seaweed-ingredients to reduce enteric methane emissions from pasture-based sheep, cattle, and dairy cows (SeaSolutions).” A-base $500K, Apr 2019-Mar 2022.

  3. Co-Applicant. Inglis, GD, Abbott, DW, Carrillo, C, Hodges, L, Richman, M. “Rationale-based mitigation of relevant Campylobacter jejuni strains within the broiler production continuum.” GF2 Poultry Cluster $559K, Apr 2018-Mar 2023.

  4. Co-Principal Investigator. Zandberg, WF, Abbott, DW, Inglis, GD, Boraston, AB, Smith, SP, Capicciotti, C. “Mitigation of Necrotic Enteritis by improving the Integrity of intestinal mucin through small molecules and stress management.” GlycoNet $370K, Apr 2020-Mar 2022.

  5. Co-Applicant. Brumer H, Inglis, GD (Co-PI), Abbott, DW, Sheedy, C, Boras, V. Sly, L. Title: “Development of next-generation glyco-caged drugs to treat enteric inflammatory diseases in humans and livestock.” GlycoNet $400K, Apr 2020-Mar2022.

  6. Principal Investigator. Abbott DW, Beauchemin, K, McAllister, TA, T, Thomas, D., Ortega Polo, R. Title:” Structural and functional characterization of bovine rumen microbiomes adapted to the consumption of seaweed in natural and farm-intensive systems.” Abase-AAFC $360K, Apr 2019-Mar 2022.

  7. Principal Investigator. Abbott DW, Alexander, T, Thomas, D. Title: “Evidence-based prebiotic-probiotic solutions for improving gut health and feed efficiency in cattle.” Beef and Cattle Research Council $576K, Apr 2018-Mar 2023.

  8. Principal Investigator. Abbott DW, Inglis GD, Slominski B.Title: “Discovery pipeline for rapid development of tailored fibrolytic enzymes that improve the digestibility of canola meal for monogastric livestock” Agriculture Funding Consortium. $234,600 Apr 2018-Mar 2020.

  9. Co- Principal Investigator. McAllister, TA (PI), Abbott, DW (Co-PI), Yang WZ. Title: “Development of novel feed enzymatic products to enhance ruminal digestion of low quality forage fiber.” Alberta Agriculture and Forestry. $315K, Apr 2018-Mar 2020.

Research and/or project statements

Agricultural Glycomics

Functional Genomics of Carbohydrate Utilization

Intestinal Microbiomes

Carbohydrate Active Enzymes

Enzyme discovery

Alternatives to antibiotics for livestock production

Education and awards

PhD, Biochemistry University of Victoria (2005). PhD thesis awarded the University of Victoria Governor General’s Gold Medal (2006) and Nominated for NSERC Silver Medal (2006)

BSc (Great Distinction), BIology (Major), Geography (Concentration), Trinity Western University (2000).

Key publications

  1. Klassen, L., Reintjes, G., Tingley, J.P., Jones, D.R., Hehemann, J.H., Smith, A.D., Schwinghamer, T.D., Arnosti, C., Jin, L., Alexander, T.W., Amundsen, C., Thomas, D., Amann, R., McAllister, T.A., Abbott, D.W. (2021). Quantifying fluorescent glycan uptake to elucidate strain-level variability in foraging behaviors of rumen bacteria, 9(1), http://dx.doi.org/10.1186/s40168-020-00975-x

    2021 - View publication details

  2. Tingley, J.P., Low, K.E., Xing, X., Abbott, D.W. (2021). Combined whole cell wall analysis and streamlined in silico carbohydrate-active enzyme discovery to improve biocatalytic conversion of agricultural crop residues, 14(1), http://dx.doi.org/10.1186/s13068-020-01869-8

    2021 - View publication details

  3. Douglas Inglis, G., Wright, B.D., Sheppard, S.A., Wade Abbott, D., Oryschak, M.A., Montina, T. (2021). Expeller-pressed canola (Brassica napus) meal modulates the structure and function of the cecal microbiota, and alters the metabolome of the pancreas, liver, and breast muscle of broiler chickens, 11(2), 1-23. http://dx.doi.org/10.3390/ani11020577

    2021 - View publication details

  4. Abbott, D.W., Aasen, I.M., Beauchemin, K.A., Grondahl, F., Gruninger, R., Hayes, M., Huws, S., Kenny, D.A., Krizsan, S.J., Kirwan, S.F., Lind, V., Meyer, U., Ramin, M., Theodoridou, K., Soosten, D.V., Walsh, P.J., Waters, S., Xing, X. (2020). Seaweed and seaweed bioactives for mitigation of enteric methane: Challenges and opportunities, 10(12), 1-28. http://dx.doi.org/10.3390/ani10122432

    2020 - View publication details

  5. Gruninger, R.J., Nguyen, T.T.M., Reid, I.D., Yanke, J.L., Wang, P., Abbott, D.W., Tsang, A., McAllister, T. (2018). Application of transcriptomics to compare the carbohydrate active enzymes that are expressed by diverse genera of anaerobic fungi to degrade plant cell wall carbohydrates, 9(JUL), http://dx.doi.org/10.3389/fmicb.2018.01581

    2018 - View publication details

  6. Brown, K., Abbott, D.W., Uwiera, R.R.E., Inglis, G.D. (2018). Removal of the cecum affects intestinal fermentation, enteric bacterial community structure, and acute colitis in mice, 9(3), 218-235. http://dx.doi.org/10.1080/19490976.2017.1408763

    2018 - View publication details

  7. Luis, A.S., Briggs, J., Zhang, X., Farnell, B., Ndeh, D., Labourel, A., Baslé, A., Cartmell, A., Terrapon, N., Stott, K., Lowe, E.C., McLean, R., Shearer, K., Schückel, J., Venditto, I., Ralet, M.C., Henrissat, B., Martens, E.C., Mosimann, S.C., Abbott, D.W., Gilbert, H.J. (2018). Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides, 3(2), 210-219. http://dx.doi.org/10.1038/s41564-017-0079-1

    2018 - View publication details

  8. Grondin, J.M., Tamura, K., Déjean, G., Abbott, D.W., Brumer, H. (2017). Polysaccharide utilization loci: Fueling microbial communities, 199(15), http://dx.doi.org/10.1128/JB.00860-16

    2017 - View publication details

  9. Jiminez, J.A., Uwiera, T.C., Wade Abbott, D., Uwiera, R.R.E., Inglis, G.D. (2017). Butyrate supplementation at high concentrations alters enteric bacterial communities and reduces intestinal inflammation in mice infected with Citrobacter rodentium, 2(4), http://dx.doi.org/10.1128/mSphere.00243-17

    2017 - View publication details

  10. Buchanan, C.J., Webb, A.L., Mutschall, S.K., Kruczkiewicz, P., Barker, D.O.R., Hetman, B.M., Gannon, V.P.J., Abbott, D.W., Thomas, J.E., Inglis, G.D., Taboada, E.N. (2017). A genome-wide association study to identify diagnostic markers for human pathogenic campylobacter jejuni strains, 8(JUN), http://dx.doi.org/10.3389/fmicb.2017.01224

    2017 - View publication details

  11. Ndeh, D., Rogowski, A., Cartmell, A., Luis, A.S., Baslé, A., Gray, J., Venditto, I., Briggs, J., Zhang, X., Labourel, A., Terrapon, N., Buffetto, F., Nepogodiev, S., Xiao, Y., Field, R.A., Zhu, Y., O'Neill, M.A., Urbanowicz, B.R., York, W.S., Davies, G.J., Abbott, D.W., Ralet, M.-C., Martens, E.C., Henrissat, B., Gilbert, H.J. (2017). Complex pectin metabolism by gut bacteria reveals novel catalytic functions, 544(7648), 65-70. http://dx.doi.org/10.1038/nature21725

    2017 - View publication details

  12. Jiminez, J.A., Uwiera, T.C., Abbott, D.W., Uwiera, R.R.E., Inglis, G.D. (2016). Impacts of resistant starch and wheat bran consumption on enteric inflammation in relation to colonic bacterial community structures and short-chain fatty acid concentrations in mice, 8(1), http://dx.doi.org/10.1186/s13099-016-0149-6

    2016 - View publication details

  13. Hobbs, J.K., Lee, S.M., Robb, M., Hof, F., Barr, C., Abe, K.T., Hehemann, J.H., McLean, R., Abbott, D.W., Boraston, A.B. (2016). KdgF, the missing link in the microbial metabolism of uronate sugars from pectin and alginate, 113(22), 6188-6193. http://dx.doi.org/10.1073/pnas.1524214113

    2016 - View publication details

  14. McLean, R., Hobbs, J.K., Suits, M.D., Tuomivaara, S.T., Jones, D.R., Boraston, A.B., Abbott, D.W. (2015). Functional analyses of resurrected and contemporary enzymes illuminate an evolutionary path for the emergence of exolysis in polysaccharide lyase family, 290(35), 21231-21243. http://dx.doi.org/10.1074/jbc.M115.664847

    2015 - View publication details

  15. Cuskin, F., Lowe, E.C., Temple, M.J., Zhu, Y., Cameron, E.A., Pudlo, N.A., Porter, N.T., Urs, K., Thompson, A.J., Cartmell, A., Rogowski, A., Hamilton, B.S., Chen, R., Tolbert, T.J., Piens, K., Bracke, D., Vervecken, W., Hakki, Z., Speciale, G., Munoz-Munoz, J.L., Day, A., Peña, M.J., McLean, R., Suits, M.D., Boraston, A.B., Atherly, T., Ziemer, C.J., Williams, S.J., Davies, G.J., Abbott, W.D., Martens, E.C., Gilbert, H.J. (2015). Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism, 517(7533), 165-169. http://dx.doi.org/10.1038/nature13995

    2015 - View publication details

  16. Abbott, D.W., Martens, E.C., Gilbert, H.J., Cuskin, F., Lowe, E.C. (2015). Coevolution of yeast mannan digestion: Convergence of the civilized human diet, distal gut microbiome, and host immunity, 6(5), 334-339. http://dx.doi.org/10.1080/19490976.2015.1091913

    2015 - View publication details

  17. Abbott, D.W., Farnell, B.J., and Yamashita, J.W. (2014). "Pectins: structure, function, and the human distal gut microbiota.", - Pectin: Chemical Properties, Uses and Health Benefits, Nova Publishers.

    2014 - View publication details

  18. Thomas, D.K., Lone, A.G., Brent Selinger, L., Taboada, E.N., Uwiera, R.R.E., Wade Abbott, D., Douglas Inglis, G. (2014). Comparative variation within the genome of Campylobacter jejuni NCTC 11168 in human and murine hosts, 9(2), http://dx.doi.org/10.1371/journal.pone.0088229

    2014 - View publication details

  19. Abbott, D.W. and Lammerts van Bueren, A. (2014). "Using structure to inform carbohydrate binding module function.", Current Opinion in Structural Biology, 28, pp. 32-40. doi : 10.1016/j.sbi.2014.07.004

    2014 - View publication details

  20. Abbott, D.W., Thomas, D., Pluvinage, B., Boraston, A.B. (2013). An ancestral member of the polysaccharide lyase family 2 displays endolytic activity and magnesium dependence, 171(7), 1911-1923. http://dx.doi.org/10.1007/s12010-013-0483-9

    2013 - View publication details

Research facility

5403 1st Avenue South
Lethbridge, AB T1J 4B1
Canada

Affiliations

University of Lethbridge, Departments of Biological Sciences; and Chemistry and Biochemistry

Canadian Glycomics Network (Investigator)

Alberta Glycomics Centre (Associate Investigator)

Language

English