Optimization of fermentation conditions for Chinese fermentation paocai with Enterococcus faecium IN3531 as a starter by single factor method

The strain Enterococcus faecium IN3531, a bacteriocin producer, was used as a starter culture for traditional fermentation paocai in china. By single factor experiment, the technological conditions of Chinese fermentation paocai using Enterococcus faecium IN3531 as a starter were optimizated, and parameters of optimization included the initial salt concentration, the inoculum size, the fermentation temperature and the ratio of material to liquid. The research results showed that, the suitable fermentation conditions were the initial salt concentration of 2%, the inoculum size of 3%, the fermentation temperature of 37°C, the ratio of material to liquid of 30%, fermentation time of 108 hours. Titratable acidity in fermentation terminus was 0.80%. © (2014) Trans Tech Publications, Switzerland.