Association Between Tail-Biting and Intestinal Microbiota Composition in Pigs
Citation
Rabhi, N., Thibodeau, A., Côté, J.-C., Devillers, N., Laplante, B., Fravalo, P., Larivière-Gauthier, G., Thériault, W.P., Faucitano, L., Beauchamp, G., Quessy, S.Association Between Tail-Biting and Intestinal Microbiota Composition in Pigs (2020) Frontiers in Veterinary Science, 7, art. no. 563762. DOI: 10.3389/fvets.2020.563762
Plain language summary
Tail-biting (TB) in pigs is a serious behavioral disorder. It is an important challenge in swine production as it impacts animal welfare and health and the economics and safety of the pork meat supply chain. To prevent TB, approaches including enrichment material and tail docking are proposed but none are optimal. Nutrition appears to be an important factor in TB behavior, perhaps by modulating the intestinal microbiota (IM). Our aim was to assess the association between TB behavior and IM in pigs through comparisons of IM in groups of biter, bitten and non-biter/non-bitten pigs (control). Our results provide the first evidence of a relationship between the occurrence of TB in biters and bitten pigs and IM. Using cortisol level as a marker, we showed that biter and bitten pigs were stressed in comparison to the control group. Interestingly, we showed that control pigs’ IM had more Lactobacillus in their IM than biters or bitten pigs. This is consistent with human and mice studies on the relationship between behavioral disorders and microbiota composition. However, further studies are needed to better understand of the cause-effect relationship between TB and IM.
Abstract
Tail-biting (TB) in pigs is a serious behavioral disorder. It is an important challenge in swine production as it impacts animal welfare and health and the economics and safety of the pork meat supply chain. To prevent TB, approaches including enrichment material and tail docking are proposed but none are optimal. Nutrition appears to be an important factor in TB behavior, perhaps by modulating the intestinal microbiota (IM). Our aim was to assess the association between TB behavior and IM in pigs through comparisons of IM in groups of biter, bitten and non-biter/non-bitten pigs. Each group composed of 12 pigs was formed at the beginning of the growing/finishing phase based on a target behavior analysis centered on TB behavior for the biter group and a score of damages caused to the tail for the bitten group. Blood and fecal samples were collected from each pig
during a TB episode, at time 0, t0, and when the TB episode was considered finished, 4 weeks later, at time 1, t1. Serum cortisol level was determined by ELISA and used as an indicator of stress. The pig’s fecal microbiota was analyzed from DNA extracted from freshly collected fecal matter using amplicon sequencing of the V4 hypervariable region of the 16S rRNA gene. Serum cortisol levels were significantly higher in either the biter or bitten pig groups compared to the negative control group (p = 0.02 and p = 0.01, respectively). The microbiota alpha-diversity was not significantly different between all groups, biter, bitten and negative control. Analyses of beta-diversity, however, revealed a significant difference between either the biter or the bitten group in comparison to the non-biter/non-bitten negative control group in terms of structure and composition of the microbiota. Lactobacillus were significantly more abundant in the negative control group compared to the two other groups (p = 0.001). No significant difference was revealed between the biter and bitten groups. Quantitative real-time PCR (qPCR) confirmed that lactobacilli were more abundant in the negative control group. Our study indicates that TB behavior is associated with the IM composition in pigs.