Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications
Citation
Bhatwa A, Wang W, Hassan YI,
Abraham N, Li X-Z and Zhou T (2021)
Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications. Front. Bioeng. Biotechnol. 9:630551.
doi: 10.3389/fbioe.2021.630551
Résumé en langage clair
Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for producing soluble and functional recombinant proteins. To overcome this hurdle, different strategies have been developed through adjusting growth conditions, engineering host strains of E. coli, altering expression vectors, and modifying the proteins of interest. These approaches will be comprehensively highlighted with some of the new developments in this review. Additionally, the unique features of protein inclusion bodies, the mechanism and influencing factors of their formation, and their potential advantages will also be discussed.
Résumé
Recombinant proteins are becoming increasingly important for industrial applications,
where Escherichia coli is the most widely used bacterial host for their production.
However, the formation of inclusion bodies is a frequently encountered challenge
for producing soluble and functional recombinant proteins. To overcome this hurdle,
different strategies have been developed through adjusting growth conditions,
engineering host strains of E. coli, altering expression vectors, and modifying the
proteins of interest. These approaches will be comprehensively highlighted with some of
the new developments in this review. Additionally, the unique features of protein inclusion
bodies, the mechanism and influencing factors of their formation, and their potential
advantages will also be discussed.