Building a phylogeny for the Adelgidae using shotgun phylogenomics and genome sequences
Citation
Brunet, B.M.T., Burke, G.R., Foottit, R.G., Havill, N.P., Johnston, S.J., and C. von Dohlen. Building a phylogeny for the Adelgidae using shotgun phylogenomics and genome sequences of the Hemlock and Balsam Woolly Adelgdids. Joint Annual Meeting of the Entomological Society of Canada, the Acadian Entomological Society, and the Canadian Society for Ecology and Evolution, Fredericton, New Brunswick, Canada, Aug. 18-22, 2019.
Résumé en langage clair
Draft genome sequences were presented for two aphid pest species in Canada, as well as results from genetic analyses for eight closely related species to characterize their evolutionary relationships.
Résumé
The Adelgidae (Hemiptera: Sternorrhynch: Aphidomorpha) contains two regulated pest species in Canada, the Hemlock (Adelges tsugae) and Balsam (Adelges piceae) Woolly Adelgids. These species cause significant mortality and reduced growth to hemlock and true fir species, respectively, in eastern and western North America. Despite a surge in genome sequencing of non-model systems over the last decade, the genomes of these species have managed to avoid attention. Recent molecular work has focused more on characterizations of adelgid symbionts, including the genome sequences of two A. tsugae obligate endosymbionts. In an effort to bolster genomic resources for A. tsugae and A. piceae, we use a combination of Illumina short-read and 10x linked read sequences to de novo assemble the first draft genomes for these species. Subsequent alignment of low-coverage short-read data for additional adelgid species/lineages will allow for investigation of the evolutionary relationships within the Adelgidae using phylogenomic methods based on orthologous gene regions.