Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176

Citation

Mainali, H.R., Vadivel, A.K.A., Li, X., Gijzen, M., Dhaubhadel, S. (2017). Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176, 7 http://dx.doi.org/10.1038/srep39550

Plain language summary

In this manuscript we describe characterization of GmCYP1, covering its chromosomal location, gene structure, conserved motif, tissue-specific expression patterns, putative subcellular location, and its interaction with the isoflavonoid regulators, GmMYB176 and 14-3-3 proteins. Our results suggest that GmCYP1 functions as a suppressor of defense in soybean via isoflavonoids.

Abstract

Cyclophilins (CYPs) belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. They catalyze the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. A yeast-two-hybrid screening using the isoflavonoid regulator GmMYB176 as bait identified GmCYP1 as one of the interacting proteins in soybean embryos. GmCYP1 localizes both in the nucleus and cytoplasm, and interacts in planta with GmMYB176, in the nucleus, and with SGF14l (a soybean 14-3-3 protein) in the nucleus and the cytoplasm. GmCYP1 contains a single cyclophilin-like domain and displays a high sequence identity with other plant CYPs that are known to have stress-specific function. Tissue-specific expression of GmCYP1 revealed higher expression in developing seeds compared to other vegetative tissues, suggesting their seed-specific role. Furthermore, GmCYP1 transcript level was reduced in response to stress. Since isoflavonoids are involved in plant stress resistance against biotic and abiotic factors, the interaction of GmCYP1 with the isoflavonoid regulators GmMYB176 and 14-3-3 protein suggests its role in defense in soybean.

Publication date

2017-01-11