Molecular analysis of three new Cherry mottle leaf virus isolates reveals intra-species genetic diversity and inter-species gene transfer
Citation
Su, L., Bhagwat, B., Bernardy, M., Wiersma, P.A., Cheng, Z., Xiang, Y. (2016). Molecular analysis of three new Cherry mottle leaf virus isolates reveals intra-species genetic diversity and inter-species gene transfer. Phytoparasitica, [online] 44(4), 571-574. http://dx.doi.org/10.1007/s12600-016-0548-8
Plain language summary
Cherry mottle leaf virus (CMLV) is a pathogen of sweet cherry, ornamental flowering cherry, peach and apricot, affecting fruit quality and quantity. Only two isolates of the virus were reported previously. Progress in understanding the origins, divergence, evolution and pathogenicity of the virus has been slow at least partially due to the lack of new virus isolates for comparative study. Here we reported the complete genome sequences of three new CMLV isolate conserved in the Canadian Plant Virus Collections. Their genome sequences show significant difference with each other. In addition the data suggest possible inter-species gene transfer among the virus species in the genus Trichovirus, in which CMLV is one of the species. These findings provide insight into the understanding of the divergence and evolution of the CMLV and other trichoviruses, which should contribute to design methods for detecting and managing the virus disease.
Abstract
Cherry mottle leaf virus (CMLV) is a pathogen of sweet cherry and several other plant species in the genus Prunus, but only two isolates of the virus were reported previously. Here we determined the complete genome sequences of three CMLV isolates that were collected during 1980s. The coat protein of the CMLV was estimated to be about 22 kDa by protein western blot assay, and agree with the size expected based on their genome sequences. The three isolates share 78.4 to 82.7 % identities at the nucleotide sequence level between each other and 78.7 to 84.3 % identities to the two previously published CMLV isolates SA1162-21 and 95CI215. The high genome sequence divergences among the CMLV isolates were supported by phylogeny analysis. Possible inter-species gene transfer between CMLV and the other species in the genus Trichovirus was revealed by the RDP recombination analysis algorithms.