Genomic strategies to precisely identify genes contributing to FHB resistance in AAC Tenacious

Fusarium head blight is considered as the most serious disease affecting wheat (Triticum aestivum L.), causing significant yield losses worldwide. Yield loss results mainly by reduction in grain quality due to shriveled and/or discolored kernels, which are referred to as Fusarium-damaged kernels (FDK) and contamination of grain with the trichothecene mycotoxin deoxynivalenol (DON). Over the past decade, significant progress has been made in improving FHB resistance and disease management, however, our understanding of resistance is still incomplete. In fact, only a single registered bread wheat cultivar, AAC Tenacious, is currently rated as resistant in Canada. We developed a Targeting Induced Local Lesions In Genomes (TILLING) population of AAC Tenacious, that in field trials showed variation for a range of useful agronomic traits, including semi-dwarf stature and FHB-resistance. Importantly, we have identified lines that are highly susceptible to FHB, allowing for the use of genomic strategies to precisely identify genes contributing to FHB resistance in AAC Tenacious. By sequencing the genome of AAC Tenacious and comparing susceptible TILLING mutants, we can identify mutated alleles associated with FHB susceptibility. Further, germplasm, genotypic and phenotypic data of the TILLING population will be available to all wheat research programs