Successful control of fire blight: Can bacteriophages do the job?
Citation
Svircev, A.M., Anany , H., Wang, Q. and Castle, A.J. Successful control of fire blight: Can bacteriophages do the job? Fourth International Symposium on Biological Control of Bacterial Plant Diseases, Biocontrol 2019, July 9-11, Viterbo, IT , FAO pp 34-38 http://www.fao.org/3/ca5311en/ca5311en.pdf
Résumé en langage clair
The use of lytic bacteriophages for the control of the fire blight pathogen, Erwinia amylovora, exploits the ability of this biological to kill the pathogen on the blossom surface. In our system, an epiphytic bacterium, named the carrier, is infected by phages. The carrier acts as a delivery and propagation system and a biological control agent. Upon application and on the pistil surface, the carrier-phage populations increase prior to the arrival of the pathogen. During the development of this biopesticide, multiple developmental processes were employed in order to improve efficacy at field level. This includes the selection, isolation and characterization of broad host range lytic phages, selection of Pantoea agglomerans isolates as carrier, in planta bioassays and multi-year field trials. In addition, the study examined the effect of host EPS on phage infection and the presence of lysogeny in field collected pathogen and carrier. Currently, the focus is on the development of a stable formulated carrier-phage preparation and the continued of field-based trials.
Résumé
The use of lytic bacteriophages for the control of the fire blight pathogen, Erwinia amylovora, exploits the ability of this biological to kill the pathogen on the blossom surface. In our system, an epiphytic bacterium, named the carrier, is infected by phages. The carrier acts as a delivery and propagation system and a biological control agent. Upon application and on the pistil surface, the carrier-phage populations increase prior to the arrival of the pathogen. During the development of this biopesticide, multiple developmental processes were employed in order to improve efficacy at field level. This includes the selection, isolation and characterization of broad host range lytic phages, selection of Pantoea agglomerans isolates as carrier, in planta bioassays and multi-year field trials. In addition, the study examined the effect of host EPS on phage infection and the presence of lysogeny in field collected pathogen and carrier. Currently, the focus is on the development of a stable formulated carrier-phage preparation and the continued of field-based trials.