The Prevalence of Fusarium species in FHB Disease Complex and Genetic Diversity of Fusarium Poae in commercial oat fields of Manitoba.

Citation

Mourita Tabassum, Mitali Banik, Meconnen Beyene, Fouad Daayf, Xiben Wang. (2018). The Prevalence of Fusarium species in FHB Disease Complex and Genetic Diversity of Fusarium Poae in commercial oat fields of Manitoba. 9th Canadian Workshop on Fusarium Head Blight (CWFHB) / 4th Canadian Wheat Symposium (CWS). Winnipeg, Manitoba, Canada, Nov 19-22, 2018.

Résumé

Fusarium head blight (FHB), is a devastating fungal disease causes by Fusarium Species. It causes dramatic yield losses and mycotoxin contamination in commercial cereal production. Moreover, trichothecenes type-A(T-2 and HT-2) and type-B(DON and NIV) are the most common group of mycotoxins produced by Fusarium species and recognized as the most acute poisoning mycotoxins. According to literature, FHB was common in oat crops surveyed in southern Manitoba in 2002 and 2003. So, this study emphasis on the prevalence of Fusarium species in commercial Oat field and another notable interest is to identify the genetic diversity of Fusarium poae. Where, F.poae is known to generate both type A and type B trichothecenes. To execute this, samples was collected from 2016 to 2018 and examined for the presence of the following pathogens: Fusarium graminearum, Fusarium poae, Fusarium avenaceum, and Fusarium sporotrichioides through morphological and molecular (conventional and real-time PCR) analysis. Besides this, mycotoxin analysis will be done by using LC-MS/MS and investigate the correlation with quantified Fusarium DNA. The morphological analysis demonstrated that Fusarium poae is the most dominant species in Fusarium cluster. In addition, a phylogenetic approach was used through bioinformatics tools to investigate the relationship between Fusarium poae isolates and TRI genes. It is expected that the TRI genes detection in F. poae may have any key gene related to the production of both mycotoxins in the trichothecene group. This study could be an important asset for evolving disease management methods and providing future information for breeding programmes in Canada.

Date de publication

2018-11-19

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