Manitoba lingonberry supplementation improves lipid profile in metabolic disorder


Madduma Hewage, S., Sid, V., Prashar, S., Shang, Y., O, K. and Siow, Y.L. (2018) Manitoba lingonberry supplementation improves lipid profile in metabolic disorder. St. Boniface Albrechtsen Research Centre R30 Conference, Winnipeg, April 21, 2018


Introduction: Hyperlipidemia is an important risk factor for developing atherosclerosis, a major cause of cardiovascular disease (CVD). Excessive dietary fat intake can cause hyperlipidemia, obesity and non-alcoholic fatty liver disease. Nutritional regulation is emerging as an alternative strategy for prevention of CVD. High anthocyanin-containing lingonberry is an evergreen shrub that grows in northern regions of North America and Europe. Our previous study reported that Manitoba lingonberry extract could protect cardiomyocytes against ischemia-reperfusion induced apoptosis. In the present study, we investigated the impact of lingonberry supplementation on attenuating CVD risk induced by chronic consumption of high-fat diet.

Methods: Mice (C57BL/6J) were fed for 12 weeks a control diet (10% kcal fat), a high-fat diet (60% kcal fat), or a high-fat diet supplemented with lingonberry extract. Liver function was assessed by measuring plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Liver histology was examined by hemotoxylin and eosin (H&E) and Oil-Red-O staining. The cholesterol and triacylglycerol levels in the plasma and liver were determined by using commercial kits.

Results: High-fat diet feeding for 12 weeks caused a significant body weight gain, hepatic lipid accumulation (fatty liver) and increased plasma lipid levels (total cholesterol, triacylglycerol). The phenotype developed in high-fat diet fed mice resembled metabolic syndrome that could increase CVD risk. Mice fed a high-fat diet supplemented with lingonberry extract displayed improved histological integrity of the liver, less hepatic lipid accumulation, decreased levels of lipids, ALT and AST in the plasma.

Conclusion: Chronic consumption of high-fat diet induces metabolic syndrome like phenotype in a mouse model. Dietary supplementation of lingonberry extract can improve lipid profiles, which may account for the protective effect of dietary berry compounds for cardiovascular disease.

Funding source: This work was supported, in part, by the Natural Sciences and Engineering Research Council of Canada and AAFC.

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