Improved Markers for a Pre-Harvest Sprouting QTL on Wheat Chromosome 3B.

Citation

Jorden M, You FM (2018) Improved markers for a pre-harvest sprouting QTL on wheat chromosome 3B. Proc 26th Plant and Animal Genome Conference, San Diego, CA, January 13-17, P1042 (poster)

Résumé

A QTL for sprouting index on chromosome 3B was identified in the AC Domain x RL4452 bi-parental mapping population (Cabral et al. 2014, BMC Plant Biology 14:340). The flanking markers derived from the Illumina iSelect 90K chip define a region of 185 Mbp in the wheat RefSeq 1.0 genome sequence containing over 1180 annotated high confidence genes. Additional markers are needed to narrow down this region for wheat breeding purposes as well as to eventually identify candidate genes. As a first step to identify additional markers SNPs previously validated in the KASP assay were identified by locating QTL flanking markers from the 'AC Domain' x RL4452 3B map on the 'Avalon' x 'Cadenza' map. Validated KASP markers mapped on Avalon x Cadenza in the region between QTL flanking markers were chosen (http://lgcapps.com/assays/wheat/) for genotyping and mapping on the 'AC Domain' x RL4452 population. Subsequently the full genomic region between flanking markers from the IWGSC RefSeq1.0 genome sequence was used to identify SNPs between the parental genotypes in a sequence database derived from exome capture sequencing of Canadian wheat genotypes. 1492 SNPs were identified in 288 annotated genes. The SNPs will be useful to further narrow down the QTL region and provide robust markers for selection of lines with increased tolerance to pre-harvest sprouting.

Date de publication

2018-01-13

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