Genetic analysis of seed hardness trait in a black bean recombinant inbred line (ril) population

Citation

Sandhu K, You FM, Conner R, Balasubramanian P, Hou A (2016) Genetic analysis of seed hardness trait in a black bean recombinant inbred line (RIL) population. The 10th Canadian Pulse Research Workshop, Winnipeg, MB, October 25-28, 2016.

Résumé en langage clair

Seed hardness trait has a profound impact on cooking time and canning quality in dry beans. This study aims to identify the unknown genetic factors and associated molecular markers to better understand the mechanism of seed being hard to fully hydrate. A genetic population was developed from a hybrid between a hard and a soft seeded black bean lines (H68-4 and BK04-001, respectively). The materials were grown at two locations in southern Manitoba during 2014-15. Seeds from both field and greenhouse grown materials were tested for seed hardness traits. The hydration tests were conducted by soaking the seeds overnight at room temperature. Using computer software analysis, we were able to identify three gene loci that responsible for the hard seed trait. Further analysis is in progress.

Résumé

Seed hardness trait has a profound impact on cooking time and canning quality in dry beans. This study aims to identify the unknown genetic factors and associated molecular markers to better understand and tag this trait. A recombinant inbred line (RIL) population was derived from a cross between hard and soft seeded black bean parents (H68-4 and BK04-001, respectively). Ninety-two RILs and parents were grown at two locations in southern Manitoba during 2014-15. Seeds from both field and greenhouse grown RIL population were tested for seed hardness traits. The hydration coefficient and stone seed count were estimated by soaking the seeds overnight at room temperature. For mapping of genomic regions contributing to the trait, RIL population was also genotyped using genotype by sequencing (GBS) approach. The QTL mapping revealed that in addition to the major QTL on Chromosome 7 at a genomic location previously reported to affect the trait, another novel QTL with a significant effect was also detected on Chromosome 1. This study also suggests that multiple genetic factors are involved in the control of this complex trait.