Characterization of cryptic species of Botrytis associated with fruit rot of highbush blueberry and raspberry in the Fraser Valley of British Columbia
Betz, E. C. L., Burlakoti, R. R., Novinscak, A., and Sabaratnam, S. 2022. Characterization of cryptic species of Botrytis associated with fruit rot of highbush blueberry and raspberry in the Fraser Valley of British Columbia. British Columbia regional meeting, 2021/Réunion régionale de la Colombie-Britannique, 2021, Canadian Journal of Plant Pathology (Abstr.) Page 2. DOI: 10.1080/07060661.2022.2102280.
Botrytis cinerea (Pers.) is a necrotrophic plant pathogen infecting over 200 plant species worldwide. The pathogen primarily causes fruit rot in several crops including highbush blueberry and red raspberry. To understand the species composition and genetic diversity of Botrytis population from highbush blueberry and raspberry in the Fraser Valley of British Columbia, a total of 254 isolates of Botrytis (105 from raspberry and 147 from blueberry) collected from commercial highbush blueberry and raspberry farms in 2016 were characterized using PCR-based assays. These assays were used to identify the species and mating types of Botrytis. The isolates were further differentiated based on transposable elements (TEs) in B. cinerea species complex such as ‘boty’, ‘flipper’, ‘transposa’, and ‘vacuma’. Among the Botrytis isolates from the raspberry, 99 isolates were B. cinerea and 4 were B. caroliniana X.P. Li & G. Schnabel. All the blueberry isolates were confirmed as B. cinerea. Regardless of the host, the majority of B. cinerea isolates were ‘boty’ type (~52 to 53%) followed by ‘vacuma’ (35 to 37%). The frequency of TEs type and mating types varied among cultivars and locations.