Rima Menassa Ph. D.

Image Rima Menassa
Chercheure scientifique
Agriculture et Agroalimentaire Canada

Recherche et / ou projets en cours

Mise au point d’une technologie de rechange à l’administration d’antimicrobiens de niveaux subthérapeutiques et métaphylactiques dans l’industrie du bœuf (2016-2019)

Approche novatrice dans le domaine de la biologie synthétique pour la production d’anticorps et de vaccins sous-unitaires particulaires pour la vaccination par voie orale et la protection des animaux d’élevage. (2018-2021)

Élaboration d’outils de LAI au moyen d’approches génomiques et écologiques pour la lutte contre les insectes ravageurs dans les serres. (2016-2019)

Plants-pièges selon le principe de l’interférence ARN en horticulture (2018-2021)

 

Énoncés de recherches/projets

Plantes utilisées comme bioréacteurs pour la production de vaccins vétérinaires et d’anticorps à titre de solutions de rechange aux antibiotiques

Transformation du génome chloroplastique de diverses espèces de végétaux pour la production d’ARN à double brin pour la lutte contre les insectes nuisibles, et pour la production de vaccins vétérinaires

Prix et études

  • Doctorat en Génétique Moléculaire, Université McGill
  • Maitrise en phytopathologie, Université Américaine de Beyrouth
  • Baccalauréat en Agriculture, Université Américaine de Beyrouth

Principales publications

Pour une liste complète de publications, visitez: AAC en ligne.

 

Tran  HH,  Chen  B,  Chen  H,  Menassa  R,  Hao  X, Bernards  M,  Huner  NPA,  and Wang  A. 2019. Development  of  a  cucumber  green  mottle mosaic virus-based expression vector for the production in cucumber of neutralizing epitopes against a devastating animal virus. Journal of Virological Methods, doi: 10.1016/j.jviromet.2019.04.006

Saberianfar R, Chin-Fatt A,  Scott A, Henry K, Topp E, and Menassa R. 2019. Plant-produced chimeric VHH-sIgA against enterohemorrhagic E. coli intimin shows cross-serotype inhibition of bacterial adhesion to epithelial cells. Front. Plant Sci. doi: 10.3389/fpls.2019.00270

Burke WG, Kaplanoglu E, Kolotilin I, Menassa R and Donly C. 2019. RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA. Front. Plant Sci. 10:313. doi: 10.3389/fpls.2019.00313

Ramírez-Alanis IA, Renaud JB, García-Lara S, Menassa R, Cardineau GA. 2018. Transient co-expression with three O-glycosylation enzymes allows production of GalNAc-O-glycosylated Granulocyte-Colony Stimulating Factor in N. benthamiana. Plant Methods 14:98. doi: 10.1186/s13007-018-0363-y.

Saberianfar, S. & Menassa, R. 2018. Strategies to Increase Expression and Accumulation of Recombinant Proteins. In Allison R. Kermode (Ed.) Molecular Pharming: Applications, Challenges, and Emerging Areas, First Edition (pp 119-135).  John Wiley & Sons, Inc.

Khamis, Z., & Menassa, R. 2018. Porcine Epidemic Diarrhea Virus. In J. MacDonald (Ed.), Prospects of Plant-Based Vaccines in Veterinary Medicine (pp. 255-266). Cham, Switzerland: Springer International Publishing AG.

Chin-Fatt, A., Topp, E., & Menassa, R. 2018. The Benefit of a Plant-Based Cattle Vaccine for Reducing Enterohemorrhagic Escherichia Coli Shedding and Improving Food Safety. In J. MacDonald (Ed.), Prospects of Plant-Based Vaccines in Veterinary Medicine (pp. 285-310). Cham, Switzerland: Springer International Publishing AG.

Feeney M, Kittelmann M, Menassa R, Hawes C, Frigerio L. 2018. Protein storage vacuoles originate by remodelling of pre-existing vacuoles in Arabidopsis thaliana. Plant Phys. 177(1):241-254. doi: 10.1104/pp.18.00010

Ceballo Y, Tiel K, López A, Cabrera G, Pérez M, Ramos O, Rosabal Y, Montero C, Menassa R, Depicker A, Hernández A. 2017. High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza. Transgenic Res. 26 (6) :775-789. doi: 10.1007/s11248-017-0047-9.

Miletic S, Hünerberg M, Kaldis A, MacDonald J, Leuthreau A, McAllister T, Menassa R.  2017. A Plant-Produced Candidate Subunit Vaccine Reduces Shedding of Enterohemorrhagic Escherichia coli in Ruminants. Biotechnol J. 12(10). doi: 10.1002/biot.201700405. Epub 2017 Sep 28.

Reuter LJ, Shahbazi MA, Mäkilä EM, Salonen JJ, Saberianfar R, Menassa R, Santos HA, Joensuu JJ, Ritala A. 2017. Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells. Bioconjug Chem.28(6):1639-1648. doi:10.1021/acs.bioconjchem.7b00075.

Zhu H, Reynolds LB, Menassa R. 2017. A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates. BMC Biotechnol. 17(1):53. doi: 10.1186/s12896-017-0372-3.

Saberianfar R, Menassa R. 2017 Protein bodies - How the ER deals with high accumulation of recombinant proteins. Plant Biotechnol J. 15(6):671-673. doi: 10.1111/pbi.12730.

MacDonald J, Miletic S, Gaildry T, Chin-Fatt A, Menassa R. 2017 Co-expression with the Type 3 Secretion Chaperone CesT from Enterohemorrhagic E. coli Increases Accumulation of Recombinant Tir in Plant Chloroplasts. Front Plant Sci. 8:283. doi: 10.3389/fpls.2017.00283.

Arthur LL, Chung JJ, Jankirama P, Keefer KM, Kolotilin I, Pavlovic-Djuranovic S, Chalker DL, Grbic V, Green R, Menassa R, True HL, Skeath JB, Djuranovic S. 2017 Rapid generation of hypomorphic mutations. Nat Commun. 8:14112. doi: 10.1038/ncomms14112

Saberianfar R, Sattarzadeh A, Joensuu JJ, Kohalmi, SE, Menassa R. 2016. Protein bodies in leaves actively exchange contents through the endoplasmic reticulum. Front Plant Sci. 7:693. doi: 10.3389/fpls.2016.00693

Topp E, Irwin R, McAllister T, Lessard M, Joensuu JJ, Kolotilin I, Conrad U, Stöger E, Mor T, Warzecha H, Hall JC, McLean MD, Cox E, Devriendt B, Potter A, Depicker A, Virdi V, Holbrook L, Doshi K, Dussault M, Friendship R, Yarosh O, Yoo HS, MacDonald J, Menassa R. 2016. The case for plant-made veterinary immunotherapeutics. Biotechnol Adv. 34(5):597-604. doi: 10.1016/j.biotechadv.2016.02.007.

Miletic SP, Simpson DJ, Szymanski CM, Deyholos MK, Menassa R. 2016.  A plant-produced bacteriophage tailspike protein for the control of Salmonella.  Front Plant Sci. 6:1221. doi: 10.3389/fpls.2015.01221.

MacDonald J, Doshi K, Dussault M, Hall JC, Holbrook L, Jones G, Kaldis A, Klima CL, Macdonald P, McAllister T, McLean MD, Potter A, Richman A, Shearer H, Yarosh O, Yoo HS, Topp E, Menassa R. 2015. Bringing plant-based veterinary vaccines to market: Managing regulatory and commercial hurdles. Biotechnol Adv. 33(8):1572-81. doi: 10.1016/j.biotechadv.2015.07.007.

Duwadi K, Chen L, Menassa R, Dhaubhadel S. 2015. Identification, Characterization and Down-Regulation of Cysteine Protease Genes in Tobacco for Use in Recombinant Protein Production. PLoS One 10(7):e0130556. doi: 10.1371/journal.pone.0130556.

Sattarzadeh A, Saberianfar R, Zipfel WR, Menassa R, Hanson MR. 2015. Green to red photoconversion of GFP for protein tracking in vivo. Scientific Reports 2015. 5:11771. doi: 10.1038/srep11771.

Saberianfar R, Joensuu JJ, Conley AJ, Menassa R. 2015. Protein body formation in leaves of Nicotiana benthamiana: a concentration-dependent mechanism influenced by the presence of fusion tags. Plant Biotechnol J. 13(7):927-37. doi: 10.1111/pbi.12329

Kolotilin I, Topp E, Cox E, Devriendt B, Conrad U, Joensuu J, Stöger E, Warzecha H, McAllister T, McLean MD, Hall JC, Menassa R. 2014. Plant-based solutions for veterinary immunotherapeutics and prophylactics. Vet. Res. 45:117. doi: 10.1186/s13567-014-0117-4.

Pereira EO, Kolotilin I, Conley AJ, Menassa R. 2014. Production and characterization of in planta transiently produced polygalacturanase from Aspergillus niger and its fusions with hydrophobin or ELP tags. BMC Biotechnol. 14:59.

Feeney M, Frigerio L, Kohalmi SE, Cui Y, Menassa R. 2013. Reprogramming cells to study vacuolar development. Frontiers in Plant Sci. - Plant Cell Biology. doi: 10.3389/fpls.2013.00493. Review.

Pereira EO, Tsang A, McAllister T, Menassa R. 2013. The production and characterization of a new active lipase from Acremonium alcalophilum using a plant bioreactor. Biotechnology for Biofuels 6(1):111

Gutierrez SP, Saberianfar R, Kohalmi S, Menassa R*. 2013. Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins. BMC Biotechnol. 13:40.

Feeney M, Frigerio L, Harada JJ, Cui Y, Menassa R*. 2013. Following Vegetative to Embryonic Developmental Transitions by Over-expressing LEAFY COTYLEDON2 in Leaves of Arabidopsis thaliana. Plant Phys. 162(4):1881-96

Kolotilin I, Kaldis A, Pereira E, Laberge S, Menassa R*. 2013. Diverse Effects of Chloroplast Expression Cassette Configuration on Transplastomic Production of Hemicellulases in Tobacco. Biotechnology for Biofuels 6(1):65.

Chen L, Dempsey BR, Gyenis L, Menassa R, Brandle JE, Dhaubhadel S*. 2013. Identification of the factors that control synthesis and accumulation of a therapeutic protein, human immune-regulatory interleukin-10 in Arabidopsis thaliana. Plant Biotechnol J. 2013 11(5):546-54

Kaldis A, Ahmad A, Reid A, McGarvey B, Brandle J, Ma S, Jevnikar A, Kohalmi SE, Menassa R. 2013. High level production of human interleukin-10 fusions in tobacco cell suspension cultures. Plant Biotechnol J. 11(5):535-45

Kolotilin I, Kaldis A, Devriendt B, Joensuu J, Cox E, Menassa R. 2012. Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco. PLoS One.7(8):e42405.

Affiliations

Membre de la International Society for Plant Molecular Farming

Membre du conseil d’administration de la Canadian Association for Plant Biotechnology

Conseil de Directeurs de Plant Canada

Professeur auxiliaire, Département de biologie, Université Western, London (Ontario)

Langue

Anglais
Français