Raman Dhariwal, M.Sc. (Ag.), M.Phil., Ph.D.

Image Raman Dhariwal
Chercheur biologique

Découverte de caractères et sélection du blé de printemps et du triticale à l’interface de la biologie moléculaire, de la génétique, de la génomique, de l’analyse statistique et de la bioinformatique modernes.

Recherche et / ou projets en cours

Développement de marqueurs génétiques faciles à utiliser pour la sélection.

Cartographie des QTL et des gènes pour la résistance aux maladies des céréales et les caractères agronomiques.

Mise au point de cultivars de blé de printemps des Prairies canadiennes à haut rendement pour l’Ouest canadien. 

Énoncés de recherches/projets

Soutien du programme de recherche sur la sélection du blé de printemps et du triticale.

Identification et caractérisation de la résistance aux maladies chez le blé de printemps et le triticale.

Activités professionnelles / intérêts

Mes travaux de recherche actuels portent sur l’identification et la caractérisation moléculaire des gènes et des QTL qui rendent les plants de blé plus résilients face à divers stress biotiques. J’essaie plus particulièrement de comprendre la base génétique et moléculaire de la résistance et des autres mécanismes de lutte contre diverses maladies dévastatrices (p. ex. brûlure de l’épi causée par le fusarium, charbon nu, carie et rouille des céréales) et divers insectes (p. ex. tenthrède) d’importance pour le blé canadien.

Prix et études

Études

Doctorat en génétique et en sélection végétale, Université Chaudhary Charan Singh, 2012

 

Prix et distinctions

Bourse d’études postdoctorales

Université de l’Alberta

Juillet 2016

 

Bourse de recherche scientifique du CRSNG

Conseil de recherches en sciences naturelles et en génie du Canada

Juillet 2013

 

Prix du meilleur exposé oral

Université agricole du Punjab

Conférence internationale

Novembre 2012

 

Bourse de recherche de la NSF

National Science Foundation (NSF), États-Unis

Mai 2011

 

Bourse principale de recherche du DBT

Département de biotechnologie (DBT), gouvernement de l’Inde

Décembre 2009

 

Représentant des étudiants en recherche de l’année 2009

Université Chaudhary Charan Singh, Inde

Janvier 2009

 

Étudiant le plus haut classé de la cohorte de diplômés M. Ph. de 2008

Université Chaudhary Charan Singh, Inde

Juillet 2008

 

Bourse de recherche du DBT pour jeunes chercheurs

Département de biotechnologie (DBT), gouvernement de l’Inde

Décembre 2007

 

Étudiant le plus haut classé de la cohorte de diplômés M. Sc. de 2007

Université Chaudhary Charan Singh, Inde

Juillet 2007

Expérience et / ou de travail international

Université agricole du Punjab, Inde

Projet ou étude :

BREAD (recherche fondamentale pour favoriser le développement agricole) : un système alternatif de gènes de nanisme pour améliorer la tolérance au stress abiotique chez les céréales

De mai 2011 à juin 2013

 

Université Chaudhary Charan Singh, Inde

Projet ou étude :

Analyse de l’interaction hôte-pathogène dans l’infection du blé par la rouille des feuilles : une approche transcriptomique

De décembre 2007 à mai 2011

 

Mise au point de marqueurs moléculaires basés sur la PCR pour la jute

De décembre 2007 à mars 2011

 

Caractérisation moléculaire de la signalisation de la tolérance à la sécheresse (système à deux composants)

De décembre 2007 à mars 2011

 

Sélection assistée par marqueurs (SAM) pour la résistance à la rouille des feuilles et la germination sur pied chez le blé panifiable

De décembre 2007 à décembre 2009

 

Étude des aberrations chromosomiques induites par l’azoture de sodium chez le riz (Oryza sativa)

De juillet 2005 à juillet 2007

Principales publications

Research and Review Papers:

Raman Dhariwal, Maria A. Henriquez, Colin Hiebert, Curt A. McCartney, Harpinder S. Randhawa (2020) Mapping of Major Fusarium Head Blight Resistance from Canadian Wheat cv. AAC Tenacious. International Journal of Molecular Sciences 21, 4497.

Peter Brown, [RELISH Consortium, including Raman Dhariwal*], Yaoqi Zhou (2019) Large expert-curated database for benchmarking document similarity detection in biomedical literature search. Database 2019, baz085. (*Member of RELISH Consortium)

Raman Dhariwal, George Fedak, Yves Dion, Curtis Pozniak, André Laroche, François Eudes, Harpinder Singh Randhawa (2018) High density single nucleotide polymorphism (SNP) mapping and quantitative trait loci (QTL) analysis in a biparental spring triticale population localized major and minor effect fusarium head blight resistance and associated traits QTL. Genes 9, 19.

Gurcharn Singh Brar, Raman Dhariwal, Harpinder Singh Randhawa (2018) Resistance evaluation of differentials and commercial wheat cultivars to stripe rust (Puccinia striiformis) infection in hot spot regions of Canada. European Journal of Plant Pathology 152: 493-502.

Dharmendra Singh, Dhananjay Kumar, Lopamudra Satapathy, Jyoti Pathak, Saket Chandra, Adnan Riaz, Govindraj Bhaganagre, Raman Dhariwal, Manish Kumar, Kumble Vinod Prabhu, Harindra Singh Balyan, Pushpendra Kumar Gupta, Kunal Mukhopadhyay (2017) Insights of Lr28 mediated wheat leaf rust resistance: Transcriptomic approach. Gene 637: 72-89.

Harindra S Balyan, Vijay Gahlaut, Anuj Kumar, Vandana Jaiswal, Raman Dhariwal, Sandhya Tyagi, Priyanka Agarwal, Supriya Pandey, Pushpendra K Gupta (2016) Nitrogen and Phosphorus Use Efficiencies in Wheat: Physiology, Phenotyping, Genetics and Breeding. Plant Breeding Reviews 40: 167-234.

Raman Dhariwal, Vijay Gahlaut, Bhaganagare R Govindraj, Dharmendra Singh, Saloni Mathur, Shailendra Vyas, Rajib Bandopadhyay, Jitendra P Khurana, Akhilesh K Tyagi, Kumble V Prabhu, Kunal Mukhopadhyay, Harindra S Balyan, Pushpendra K Gupta (2015) Stage-specific reprogramming of gene expression characterizes Lr48-mediated adult plant leaf rust resistance in wheat. Functional & Integrative Genomics 15: 233-245.

Vijay Gahlaut, Saloni Mathur, Raman Dhariwal, Jitendra P Khurana, Akhilesh K Tyagi, Harindra S Balyan, Pushpendra K Gupta (2014) A multi-step phosphorelay two-component system impacts on tolerance against dehydration stress in common wheat. Functional & Integrative Genomics 14: 707-716.

Harindra S Balyan, Pushpendra K Gupta, Sachin  Kumar, Raman  Dhariwal, Vandana Jaiswal, Sandhya Tyagi, Priyanka Agarwal, Vijay Gahlaut and Supriya Kumari (2013) Genetic dissection and breeding for improvement of grain protein  content  and  other  health  related  constituents of wheat grain: current status and future needs. Plant Breeding 132: 446-457.

Moumita Das, Sumana Banerjee, Raman Dhariwal, Shailendra Vyas,  Reyazul R. Mir, Niladri Topdar, Avijit Kundu, Jitendra P. Khurana, Akhilesh K. Tyagi, Debabrata Sarkar, Mohit K. Sinha, Harindra S. Balyan and Pushpendra K. Gupta (2012) Development of SSR markers and construction of a linkage map in jute. Journal of Genetics 91: 21-23.

Raman Dhariwal, Gaganpreet Kaur, Johar S Saini, Kulvinder S Gill, Harindra S Balyan, Pushpendra K Gupta (2012) Identification of an alternate wheat dwarfing gene: wheat homologues of Arabidopsis Dwarf4 gene. Crop Improvement 39 (Spl. Issue): 13-14.

Gaganpreet Kaur, Raman Dhariwal, Kanwardeep Singh, Suruchi Jindal, Johar S Saini, Madhu M Jindal, Kulvinder S Gill (2012) Exploring the role of auxin influx carrier gene AUX1 for development of an improved dwarfing system in wheat. Crop Improvement 39 (Spl. Issue): 215-216.

Kanwardeep Singh, Suruchi Jindal, Raman Dhariwal, Gaganpreet Kaur, Johar Singh, Kulvinder S Gill (2012) In silico prediction and analysis of TaPIN1 (Auxin efflux carrier) gene in wheat. Crop Improvement 39 (Spl. Issue): 419-420.

Suruchi Jindal, Kanwardeep Singh, Gaganpreet Kaur, Raman Dhariwal, Kulvinder S Gill, Kuldeep Singh (2012) In silico prediction and analysis of maximum auxiliary branching (max2) gene in Triticum aestivum using comparative genomics. Crop Improvement 39 (Spl. Issue): 409-410.

Raman Dhariwal, Shailendra Vyas, Bhaganagare R Govindraj, Shailendra K Jha, Jitendra P Khurana, Akhilesh K Tyagi, Kumble V Prabhu, Harindra S Balyan and Pushpendra K Gupta (2011) Analysis of differentially expressed genes in leaf rust infected bread wheat involving seedling resistance gene Lr28. Functional Plant Biology 38: 479-492.

Jitendra Kumar, Vandana Jaiswal, Ajay Kumar, Ajay Kumar, Reazul R Mir, Sachin Kumar, Raman Dhariwal, Sandhya Tyagi,  Kumble V Parbhu, Harindra S Balyan and Pushpendra K Gupta (2011) Introgression of a major gene for high grain protein content in some Indian bread wheat cultivars. Field Crops Research 123: 226-233.

Raman Dhariwal, Shailendra Vyas, Bhaganagare R Govindraj, Shailendra K Jha, Jitendra P Khurana, Akhilesh K Tyagi, Kumble V Prabhu, Harindra S Balyan and Pushpendra K Gupta (2011) Genome-wide transcriptome analysis of seedling resistance to leaf rust conferred by Lr28 gene in bread wheat. Crop Improvement 38 (Spl. Issue): 260.

Other Publications: 

Abstracts/conference proceedings:

Raman Dhariwal, Maria Antonia Henriquez, Colin Hiebert, Curt McCartney, Robert Graf, Harpinder Randhawa (2018) Canadian heritage wheat variety alleles of flowering and plant height pathway genes associated with low Fusarium head blight. In: Proceedings of the 9th Canadian Workshop on Fusarium Head Blight /4th Canadian Wheat Symposium joint conference. Winnipeg, Manitoba, Nov 19 - 22, 2018. (Oral presentation)

Harpinder Randhawa, Gurcharn Brar, Randy Kutcher, Raman Dhariwal (2018) Stripe rust virulence in western Canada. In: Abstracts of BGRI Technical Workshop, Marrakech, Morocco, Apr 14-17, 2018.

Harpinder Randhawa, Raman Dhariwal, Maria Antonia Henriquez, Colin Hiebert, Curt McCartney (2018) Flowering pathway genes regulating Fusarium head blight resistance in wheat. In: Langin T, et al. (eds.) (2017) Book of abstracts: EUCARPIA Cereal section Meeting, Polydôme, Clermont-Ferrand, France, Mar 19h - 22, 2018.

Harpinder Randhawa, Raman Dhariwal, Maria Antonia Henriquez, Colin Hiebert, Curt McCartney (2017) Characterization of Fusarium head blight resistance in Canadian spring wheat cultivar AAC Tenacious. In: Buerstmayr H, et al. (eds.) (2017) Proceedings of the 13th International Wheat Genetics Symposium. Tulln, Austria, Apr 23 - 28, 2017. BOKU - University of Natural Resources and Life Sciences, Vienna, Austria; ISBN: 978-3-900932-48-0, p 230.

Raman Dhariwal, Harpinder Randhawa, Robert Graf, Colin Hiebert, Curt McCartney and François Eudes (2017) RNAseq bulked segregants suppression subtractive method for tagging of stem solidness genes in common wheat. In: Plant and Animal Genome Conference XXV. San Diego, USA, Jan 13 - 18, 2017.

Raman Dhariwal, Harpinder S. Randhawa and Robert J. Graf (2016) Identification and characterization of new stripe rust resistance sources in spring wheat. In: Proceedings of the 3rd Canadian Wheat Symposium. Ottawa, Canada, Nov 22 - 25, 2016. p 37-38.

Raman Dhariwal, Harpinder S. Randhawa, George Fedak, Yves Dion, François Eudes, André Laroche and Curtis Pozniak (2016) Mapping fusarium head blight resistance and DON related QTLs in Triticale. In: Miller S (ed.) 2016) Proceedings of the 8th Canadian Workshop on Fusarium Head Blight. Ottawa, Canada, Nov 20 - 22, 2016. p 43.

Raman Dhariwal, Harpinder Randhawa, George Fedak, Yves Dion, François Eudes, Andre Laroche and Curtis Pozniak (2016) Genetic mapping of disease resistance and agronomic traits using SNP markers in triticale. In: Proceedings of the 9th International Triticale Symposium. Szeged, Hungary, May 23 - 27, 2016. (Oral Presentation)

Raman Dhariwal and Harpinder Randhawa (2016) Identification and characterization of new resources of common bunt resistance in spring wheat. In: Morgounov A and Muminjanov H (eds.) (2016) Proceedings of the XIX International Workshop on Smuts and Bunts. Izmir, Turkey, May 3 - 6, 2016. p 41-42. (Oral Presentation)

Raman Dhariwal, Harpinder Randhawa, Robert Graf, Andre Laroche, Denis Gaudet (2015) Towards identification of new sources of stripe rust resistance in spring wheat. In: 36th Annual Meeting of the Plant Pathology Society of Alberta. Lethbridge, Canada, 11/2015. p 29. (Oral Presentation)

Raman Dhariwal, Harpinder Randhawa, Robert Graf, Andre Laroche, Denis Gaudet (2015) Genetic analysis of stripe rust resistance in spring wheat. In: 14th International Cereal Rusts and Powdery Mildews Conference. Helsingør, Denmark, 7/2015. p 138.

Raman Dhariwal, Harpinder S Randhawa, Curtis Pozniak, Brent McCallum, Tom Fetch, Robert Graf (2014) Genetic analysis of stem, leaf and stripe rust resistance in a spring wheat. In: the 2nd Canadian Wheat Symposium. Saskatoon, Canada, 06/2014. 

Harpinder Randhawa, Raman Dhariwal, Curtis Pozniak, Brent McCallum, Tom Fetch, Aakash Goyal, Robert Graf (2014) Genetic mapping of stem, leaf and stripe rust resistance in a spring wheat population using 90K SNP Infinium iSelect assay. In: Cereals for Food, Feed and Fuel - Challenge for Global Improvement, Wernigerode, Germany, 06/2014.

Supriya Kumari, Sachin Kumar, Raman Dhariwal, Bikram S Gill, Sunish K Sehgal, Catherine Feuillet, Pierre Sourdille, Harindra S Balyan, Pushpendra K Gupta (2014) In silico identification of a putative TaGS5, an ortholog of rice OsGS5 associated with grain size. In: Science of OMICS Agricultural Productivity: Future Perespectives, Pantnagar, India; 3/2014. 

Gaganpreet Kaur, Raman Dhariwal, Harindra S Balyan, Johar Saini, Pushpendra K Gupta, Kulvinder S Gill (2013) Exploring the role of auxin influx carrier gene Aux1 for development of improved root length in wheat. In: InterDrought-IV, Perth, Australia, 9/2013. p 154. 

Vijay Gahlaut, Saloni Mathur, Raman Dhariwal, Jitendra P Khurana, Akhilesh K Tyagi, Harindra S Balyan, Pushpendra K Gupta (2013) Identification of two-component system (TCS) candidate genes and their expression analysis under dehydration stress in wheat. In: InterDrought-IV, Perth, Australia, 9/2013. p 166. 

Pushpendra K Gupta, Harindra S Balyan, Sachin Kumar, Raman Dhariwal, Vandana Jaiswal, Sandhya Tyagi, Priyanka Agarwal, Vijay Gahlaut, Supriya Kumari (2012) Marker-assisted selection for crop improvement of some major crops in India. Plant & Animal Genomes XX Conference, Town & Country Convention Center San Diego, CA, USA, Jan 14 - 18, 2012. (Oral Presentation)

Harindra S Balyan, Sachin Kumar, Raman Dhariwal, Vandana Jaiswal, Sandhya Tyagi, Priyanka Agarwal, Vijay Gahlaut, Pushpendra K Gupta (2011) Molecular markers for genetic dissection and improvement of some grain quality traits in bread wheat. In: Development and use of molecular markers in crop improvement, Oct 29 - 31, NASC Complex, New Delhi, India. (Oral Presentation)

Raman Dhariwal, Shailendra Vyas, Bhaganagare Govindraj R, Shailendra K Jha, Jitendra P Khurana, Akhilesh K Tyagi,    Kumble V Prabhu, Harindra S Balyan and Pushpendra K Gupta (2011) Analysis of differentially expressed genes in leaf rust infected bread wheat involving seedling resistance gene Lr28. In: Hanchinal, R.R. et. al. (eds.) Proc. 3rd Int. Group Meeting on Wheat Productivity Enhancement under Changing Climate. Feb 9 - 12, 2011, UAS, Dharwad, India: p 118. 

Newsletters/Reports:

Pushpendra K Gupta, Harindra S Balyan, Jitendra Kumar, Reazul R Mir, Sachin Kumar, Raman Dhariwal, Vandana Jaiswal, Sandhya Tyagi, Priyanka Agarwal, Vijay Gahlaut, Supriya Kumar (2013) Molecular breeding, induced mutagenesis, and transcriptome analysis in wheat. Annual Wheat Newsletter 59: 20-26.

Manual:

Raman Dhariwal (2010) Virus Induced Gene Silencing (VIGS) in Bread Wheat. A hands-on-training manual.