Pankaj Bhowmik, Ph.D

Image Pankaj Bhowmik
Agent de Recherches

Chercheur scientifique, Technologies cellulaires et développement de caractères

Agent de recherches, Initiatives stratégiques en recherche

Professeur auxiliaire, Irving K. Barber School, UBC Okanagan

Recherche et / ou projets en cours

  •  Amélioration génétique des protéines, les saveurs étrangères, de la qualité antinutritionnelle et de l’aptitude à la transformation des légumineuses.
  •  Système efficace d’isolation de protoplastes végétaux et de transformation et régénération transitoires pour la modification génétique facilitée par les courtes répétitions palindromiques groupées et régulièrement espacées (CRISPR) Cas9.

Énoncés de recherches/projets

  •  Puisque la qualité des protéines du pois et d’autres légumineuses est limitée par la faible concentration en acides aminés soufrés de la méthionine et de la cystéine, l’amélioration de qualité des protéines par l’augmentation des niveaux d’acides aminés soufrés est une priorité pour les marchés nouveaux et élargis.
  • Un autre obstacle à l’utilisation accrue de produits de légumineuses dans l’industrie alimentaire est les saveurs étrangères.

Activités professionnelles / intérêts

 

  • Culture de tissus végétaux, transformation, édition génique
  • Mise en pratique des technologies cellulaires — manipulation génétique des protoplastes et des microspores pour l’amélioration des cultures

Prix et études

Ehime University — Matsuyama, Ehime | Ph.D.

Agriculture, 2004

Kagawa University Takamatsu, Kagawa | Maîtrise ès sciences

Agriculture, 2001

Bangladesh Agricultural University Mymensingh, Bangladesh | Baccalauréat ès sciences

Agriculture, 1996

 

Bourse de recherche scientifique dans les laboratoires du gouvernement canadien du CRSNG : service des bioproduits et des bioprocédés, Centre de recherche et de développement de Lethbridge, Agriculture et Agroalimentaire Canada, de septembre 2007 à août 2009 

Bourse postdoctorale Izaak Walton Killam : département des sciences de l’agriculture, des aliments et de la nutrition (AFNS), Université de l’Alberta, Canada, de janvier 2006 à août 2007 

Bourse postdoctorale de la Japanese Society for the Promotion of Science (JSPS) : d’avril 2004 à décembre 2005

 

Expérience et / ou de travail international

 

  • Boursier de la Japan Society for the Promotion of Science (JSPS) — National Agriculture Research Organization, Sapporo, Japon
  • Bangladesh Agriculture Research Institute (BARI) — Agent scientifique

Principales publications

Selected Publications:

Pandey PK, Bhowmik P, Kagale S. Optimized methods for random and targeted mutagenesis in field pea (Pisum sativum L.). Front Plant Sci. 2022 Sep 8; 13:995542. Doi: 10.3389/fpls.2022.995542.

Islam T, Afroz N, Koh C, Hoque MN, Rahman MJ, Gupta DR, Mahmud NU, Nahid AA, Islam R, Bhowmik PK and Sharpe AG. 2022. Whole-genome sequencing of a year-round fruiting jackfruit (Artocarpus heterophyllus Lam.) reveals high levels of single nucleotide variation. Front. Plant Sci., doi.org/10.3389/fpls.2022.1044420.

Nadimi, M.; Loewen, G.; Bhowmik, P.; Paliwal, J. Effect of Laser Biostimulation on Germination of Sub-Optimally Stored Flaxseeds (Linum usitatissimum). Sustainability 2022, 14, 12183.

Zaji, Z. Liu, G. Xiao, P. Bhowmik, J. S. Sangha and Y. Ruan, "Wheat spikes counting using object-level data augmentation," 2022 IEEE International Instrumentation and Measurement Technology Conference (I2MTC), Ottawa, ON, Canada, 2022, pp. 1-6, doi: 10.1109/I2MTC48687.2022.9806479.

Gao, P., Quilichini, T.D., Yang, H., Li, Q., Nilsen, K.T., Qin, L., Babic, V., Liu, L., Cram, D., Pasha, A., Esteban, E., Condie, J., Sidebottom, C., Zhang, Y., Huang, Y., Zhang, W., Bhowmik, P., Kochian, L.V., Konkin, D., Wei, Y., Provart, N.J., Kagale, S., Smith, M., Patterson, N., Gillmor, C.S., Datla, R. and Xiang, D. (2022), Evolutionary divergence in embryo and seed coat development of U’s Triangle Brassica species illustrated by a spatiotemporal transcriptome atlas. New Phytol, 233: 30-51.https://doi.org/10.1111/nph.17759

Détain, A., Bhowmik, P., Nathalie Leborgne-Castel, N., and Ochatt, S. 2022. Latest biotechnology tools and targets for improving abiotic stress tolerance in protein legumes. Environmental and Experimental Botany, 197: 104824; https://doi.org/10.1016/j.envexpbot.2022.104824.

Bhowmik, P., Konkin, D., Polowick, P., Hodgins, C. L., Subedi, M., Xiang, D., Yu, B., Patterson, N., Rajagopalan, N., Babic, V., Ro, D.‐K., Tar'an, B., Bandara, M., Smyth, S. J., Cui, Y., & Kagale, S. (2021). CRISPR/Cas9 gene editing in legume crops: Opportunities and challenges. Legume Science, 1– 16. https://doi.org/10.1002/leg3.96.

Lie, K., Seegers S., Cao, W., Wanasundara, J., Chen, J., da Silva, A.E., Ross, K., Franco, A.L., Vrijenhoek, T., Bhowmik, P., Li, Y., Wu, X., and Bloomer, S. 2021. An International Collaborative Study on Trypsin Inhibitor Assay for Legumes, Cereals, and Related Products. J Am Oil Chem Soc. DOI 10.1002/aocs.12459.

Adhikary, D., Kulkarni, M., El-Mezawy, A., Mobini, S., Elhiti, M., Gjuric, R., Ray, A., Polowick, P., Slaski, J.J., Jones, M.P., and Bhowmik, P. 2021. Medical cannabis and industrial hemp tissue culture: present status and future potential. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2021.627240.

Chakraborty, M.; Hasanuzzaman, M.; Rahman, M.; Khan, M.A.R.; Bhowmik, P.; Mahmud, N.U.; Tanveer, M.; Islam, T. Mechanism of Plant Growth Promotion and Disease Suppression by Chitosan Biopolymer. Agriculture 2020, 10, 624.

Cho, J-Y., Bhowmik, P., Patricia L. Polowick, P., Sabine G. Dodard, S., El-Bakkari, M., Nowak, G., Fenniri, H., and Hemraz, U.2020. Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes. ACS Omega 5 (38), 24422-24433.

Cram, D., Kulkarni, M., Buchwaldt, M. Rajagopalan, N., Bhowmik, P., Rozwadowski, K., Parkin, I., Sharpe, A. and Kagale, S. 2019. WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat. BMC Plant Biol 19, 474 doi: 10.1186/s12870-019-2097-z.

Islam, M., Wahid, K. A., Dinh, A. V., & Bhowmik, P. 2019. Model of dehydration and assessment of moisture content on onion using EIS. Journal of Food Science and Technology, 56(6), 2814–2824.             

Bhowmik, P., Ellison, E., Polley, B., Bollina, V., Kulkarni, M., Ghanbarnia, K., et al. 2018. Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Sci. Rep. 8:6502. 

Bhowmik, P., Hassan, M., Molla, K., Rahman, M. and Islam, T. 2018. Application of CRISPR/Cas genome editing tools for the improvement of plant abiotic tolerance. In: Approaches for Enhancing Abiotic Stress Tolerance in Plants, CRC Press, Taylor and Francis Group, USA, pp 459-472.

Rajagopalan, N., Kagale, S., Bhowmik, P., Song, H. 2018. A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies." Methods Protoc. 1, no. 2: 17.

Shunmugam, A.S.K., Bollina, V., Dukowic-Schulze, S., Bhowmik, P.K.  et al. MeioCapture: an efficient method for staging and isolation of meiocytes in the prophase I sub-stages of meiosis in wheat. BMC Plant Biol 18, 293 (2018). https://doi.org/10.1186/s12870-018-1514-z.

Haque, E., Taniguchi, H., Hassan, MM. Bhowmik, P., Karim, MR., Śmiech, M., Zhao, K., Rahman, M. and Islam, T. (2018) Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges. Front. Plant Sci. 9:617. 

Bhowmik P.K, Dirpaul J, Polowick P and Ferrie A. 2011. An efficient high throughput Brassica napus microspore culture system: Influence of percoll gradient separation and precise bud selection on embryogenesis. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-010-9913-3.

Chandra, D., Matsui T., Suzuki, H. Kosugi, Y., Fujimura, K., and Bhowmik P.K. 2010. Textural and compositional changes of stored iceberg lettuce in relation to harvest season and storage. International Journal of Vegetable Science 16: 44-59.

Basu, S.K., Dutta, M., Goyal, A., Bhowmik, P.K., Kumar, J., and S. Nandy, S. 2010. Is genetically modified crop the answer for the next green revolution? GM Crops, 1 (2): 68-79.

Aremu, M.O., Basu, S.K., Gyar, S.D., Goyal, A., Bhowmik, P.K. and Datta Banik, S. 2009. Proximate composition and functional properties of mushroom flours from Ganoderma spp., Omphalotus olearius (DC.) Sing. And Hebeloma mesophaeum (Pers.) Quél. Used in Nasarawa State, Nigeria. Mal J Nutr 15(2): 233 – 241.

Bhowmik, P.K. and Basu S. 2008. Current developments, progress, issues and concerns in producing transgenic peas (Pisum sativum L.). Transgenic Plant Journal 2(2): 138-150.

Rashid, H., Khan, M.A., Amin, A., Nawab, K., N Hussain, N., and Bhowmik, P.K. 2008. Effect of Parthenium hysterophorus L., root extracts on seed germination and growth of maize and barley. The Americas J. Plant Sci. Biotechnol 2 (2), 51-55

Bhowmik P. K., K. Tamura, Y. Sanada, K. Tase and T. Yamada. 2007. Molecular cloning and characterization of a cDNA encoding sucrose synthase from perennial ryegrass (Lolium perenne L.). Indian Journal of Botanical Research 3(2): 237-250.

Bhowmik, P. K. and T. Matsui. 2007. Changes in acid invertase and sucrose synthase activity and gene expression during storage of asparagus spears. Indian Journal of Botanical Research 3(2): 251-264.

Bhowmik P. K., K.Tamura, Y. Sanada, K. Tase and T. Yamada. 2006. Sucrose Metabolism of Perennial Ryegrass in Relation to Cold Acclimation. Zeitschrift für Naturforschung C- a Journal of Biosciences 61(c): 99-104.

Bhowmik, P. K. and T. Matsui. 2005. Ethylene biosynthetic genes in ‘Moso' bamboo shoot in response to wounding. Postharvest Biology and Technology 38 (2): 188-194.

Bhowmik, P. K. and T. Matsui. 2005. Changes in phenylalanine ammonia lyase activity and gene expression during storage of asparagus spears. Zeitschrift für Naturforschung C- a Journal of Biosciences. 60 (2): 128-132.

Bhowmik, P. K. and T. Matsui. 2004. Changes in the Activity and Expression of 1 Aminocyclopropane -1-Carboxylate (ACC) Synthase, ACC Oxidase, and Phenylalanine Ammonia-Lyase in Asparagus Spears in Response to Wound- Induced Ethylene Synthesis. HortScience 39(6):1074-1078.

Bhowmik, P.K., T. Matsui and E. Miki. 2004. α-aminooxi-β-phenylpropionic acid controls phenylalanine ammonia lyase gene expression during storage of asparagus spears. Journal of Food, Agriculture & Environment 2(1): 10-13.

Bhowmik, P. K., T. Matsui, F. G. Enriquez, A. K. M. S. Alam, H. Suzuki and Y. Kosugi. 2003. Pre- and postharvest fiber development in green asparagus as related to temperature during growth, storage, and handling. Journal of Food, Agriculture Environment 1(4): 50-53.

Bhowmik, P. K., T. Matsui, T. Ikeuchi and H. Suzuki. 2002. Changes in storage quality and shelf life of green asparagus over an extended harvest season. Postharvest Biology and Technology. 26(3): 323-328.

Bhowmik, P.K., T. Matsui, K. Kawada, and H. Suzuki. 2001. Seasonal changes of asparagus spears in relation to enzyme activities and carbohydrate content. Scientia Horticulturae. 88(1): 1-9. 

Installation de recherche

110, place Gymnasium
Saskatoon, SK S7N 0W9
Canada

Affiliations

Membre de l’exécutif de l’Association canadienne de biotechnologie des plantes (ACBP)

Site Web : http://www.canadianplantbiotech.ca/; de janvier 2014 à aujourd’hui; January, 2014 – Present

Language

Anglais

Other languages

Bengali