Frédéric Marsolais, Ph.D.

Image Frédéric Marsolais
Chercheur scientifique

Programme de recherche sur la chimie des protéines des légumineuses

Agriculture et Agroalimentaire Canada
London, Ontario

Recherche et / ou projets en cours

Les travaux de recherches portent actuellement sur la chimie des protéines des légumineuses et le métabolisme des acides aminés dans les graines.

Énoncés de recherches/projets

  • Protéines et pathologie chez le soya: approches génomiques pour améliorer le soya pour les producteurs canadiens
  • Amélioration génétique des protéines et des caractères de qualité des graines du pois
  • Métabolisme de l’asparagine et fonction de cet acide aminé dans la signalisation de l’azote

Publications récentes

Articles de recherche

  1. Xiong R, Wang A, Marsolais F, Todd C (2022) A fast and efficient method to introduce Apple Latent Spherical Virus to legume plants via Agrobacterium rhizogenes-mediated transformation of hairy roots. Legume Sci. 3, e154. doi:10.1002/leg3.154
  2. Palmer JP, Pajak A, Robson B, Zhang B, Joshi J, Diapari M, Pauls KP, Marsolais F (2021) PECTIN ACETYLESTERASE 8 influences pectin acetylation in the seed coat, seed imbibition and dormancy in common bean (Phaseolus vulgaris L.). Legume Sci 3, e130. doi:10.1002/leg3.130
  3. Luo Y, Na R, Nowak J, Qiu Y, Lu QS, Yang C, Marsolais F, Tian L. (2021) Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV. BMC Plant Biol 21, 419. doi:10.1186/s12870-021-03138-8
  4. Kambhampati S, Pajak A, Marsolais F (2021) Evidence that class I glutamine amidotransferase, GAT1_2.1, acts as a glutaminase in roots of Arabidopsis thaliana. Plant Sci 312, 111033. doi:10.1016/j.plantsci.2021.111033
  5. Fu Y-B, Cober ER, Morrison MJ, Marsolais F, Peterson GW, Horbach C (2021) Patterns of genetic variation in a soybean germplasm collection as characterized with genotyping-by-sequencing. Plants 10, 1611. doi: 10.3390/plants10081611. 
  6. Song J, Xie X, Chen C, Shu J, Thapa RK, Nguyen V, Bian S, Kohalmi SE, Marsolais F, Zou J, Cui Y (2021) Endosperm-synthesized pioneer-like transcription factor LEAFY COTYLEDON1 enables embryo maturation in Arabidopsis. Nat Commun 12, 3963. doi:10.1038/s41467-021-24234-1
  7. Viscarra-Torrico RC, Aga Pajak A, Soler Garzón A, Zhang B, Pandurangan S, Diapari M, Song Q, Conner RL, House JD, Miklas PN, Hou A, Marsolais F (2021) Common bean (Phaseolus vulgaris L.) with increased cysteine and methionine concentration. Legume Sci. e103. doi: 10.1002/leg3.103
  8. Arshad M, Puri A, Simkovich AJ, Renaud J, Gruber MY, Marsolais F, Hannoufa A (2020) Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature. BMC Genomics 21, 758. doi: 10.1186/s12864-020-07161-1
  9. Reinprecht Y, Schram L, Marsolais F, Smith TH, Hill B and Pauls KP (2020) Effects of nitrogen application on nitrogen fixation in common bean production. Front. Plant Sci. doi: 10.3389/fpls.2020.01172.
  10. Islam N, Bett KE, Pauls KP, Marsolais F, Dhaubhadel S (2020) A basic helix-loop-helix transcription factor, PvTT8, encoded by the P gene, regulates postharvest seed coat darkening in pinto bean (Phaseolus vulgaris). Plants, People, Planet 2, 663-677. doi: 10.1002/ppp3.10132.
  11. Jiang Y, MacLean DE, Perry GE, Marsolais F, Hill B, Pauls KP (2020) Evaluation of beneficial and inhibitory effects of nitrate on nodulation and nitrogen fixation in common bean (Phaseolus vulgaris). Legume Sci. 2, e45. doi: 10.1002/leg3.45.
  12. Xiong R, Pajak A, Wang A, Yoshikawa N, Marsolais F, Todd CD (2019) Agrobacterium-mediated inoculation of asymptomatic Apple latent spherical virus as gene silencing vector in pea (Pisum sativum L.). Legume Sci. 1, e14. doi: 10.1002/leg3.14.  
  13. Wilker JL, Navabi A, Rajcan I, Marsolais F, Hill B, Torkamaneh D, Pauls PK (2019) Agronomic performance and nitrogen fixation of heirloom and conventional dry bean varieties under low-nitrogen field conditions. Front Plant Sci. 10, 952. doi: 10.3389/fpls.2019.00952. Research Topic: Legumes for Global Food Security
  14. Joshi J, Saboori-Robat E, Solouki M, Mohsenpour M, Marsolais F (2019) Distribution and possible biosynthetic pathway of non-protein sulfur amino acids in legumes. J Exp Bot 70, 4115-4121. doi: 10.1093/jxb/erz291. Special issue: Sulfur nutrition: impacts on plant development, metabolism, and stress responses.
  15. Joshi J, Renaud J, Sumarah M, Marsolais F (2019) Deciphering S-methylcysteine biosynthesis in common bean by isotopic tracking with mass spectrometry. Plant J 100, 176-186. doi: 10.1111/tpj.14438 (Cover figure)
  16. Chen C, Shu J, Li C, Thapa R, Nguyen V, Yu K, Yuan Z-C; Kohalmi S, Liu J, Marsolais F, Huang S, Cui Y (2019) RNA polymerase II independent recruitment of SPT6L at transcription start sites in Arabidopsis. Nucl Acids Res 47, 6714-6725. doi: 10.1093/nar/gkz465
  17. Saboori-Robat E, Joshi J, Pajak A, Solouki M, Mohsenpour M, Renaud J, Marsolais F. (2019) Common bean (Phaseolus vulgaris L.) accumulates most S-methylcysteine as its γ-glutamyl dipeptide. Plants 8, 126. doi: 10.3390/plants8050126. Special Issue "Advances in Plant Sulfur Research"
  18. Ajewole A, Santamaria-Kisiel L, Pajak A, Jaskolski M, Marsolais F (2018) Structural basis of potassium activation in plant asparaginases. FEBS J 285, 1528-1539. doi.: 10.1111/febs.14428
  19. Duwadi K, Austin RS, Mainali HR, Bett K, Marsolais F, Dhaubhadel, S (2018) Metabolomics and transcriptomics of two pinto bean cultivars that differ in postharvest seed coat darkening reveal extensive differences in proanthocyanidin biosynthesis and accumulation. BMC Genomics 19, 260. doi: 10.1186/s12864-018-4550-z
  20. Balasubramanian PM, Rupert T, Marsolais F, Park SJ, Navabi A, Smith TH, Khanal R, Burt AJ, Pauls KP (2018) AAC Argosy navy dry bean. Can J Plant Sci 98, 199–202. doi: 10.1139/CJPS-2017-0176
  21. Balasubramanian PM, Rupert T, Marsolais F, Park SJ, Navabi A, Smith TH, Khanal R, Burt AJ, Pauls KP (2018) AAC Shock navy dry bean. Can J Plant Sci 98, 203–206 doi: 10.1139/CJPS-2017-0175
  22. Chen C, Li C, Wang Y, Renaud J, Tian G, Kambhampati S, Saatian B, Vi N, Hannoufa A, Marsolais F, Yuan Z-C, Yu K, Austin RS, Liu J, Kohalmi SE, Wu K, Huang S, Cui Y. (2017) Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis. Nat Plants 3, 814-824. doi: 10.1038/s41477-017-0023-7
  23.  

Book

  1. The Common Bean Genome (2017) Perez de la Vega M, Santalla M, Marsolais F (eds). Series Compendium of Plant Genomes. Springer International Publishing, Cham, Switzerland. doi: 10.1007/978-3-319-63526-2

Book Chapters

  1. Wiesinger JA, Marsolais F, Glahn RP (2021) Health implications and nutrient bioavailability of bioactive compounds in dry beans and other pulses. In Dry Beans and Pulses Production, Processing and Nutrition, 2nd ed. Siddiq M, Uebersax MA, eds. Wiley. pp. 505-529. doi: 10.1002/9781119776802
  2. Kambhampati S, Ajewole E, Marsolais F. (2018) Recent advances in asparagine metabolism. In Progress in Botany Vol. 79. Cánovas FM, Lüttge U, Matyssek R (eds). Springer International Publishing, Cham, Switzerland. pp. 49-74. doi: 10.1007/124_2017_13
  3. Joshi J, Pandurangan S, Diapari M, Marsolais F (2017) Comparison of gene families: seed storage proteins. In The Common Bean Genome. Perez de la Vega M, Santalla M, Marsolais F (eds). Series Compendium of Plant Genomes. Springer International Publishing, Cham, Switzerland. pp. 201-217. doi: 10.1007/978-3-319-63526-2_10
  4. Pérez de la Vega M, Santalla M, Marsolais F (2017) Prospects: the importance of common bean as a model crop. In The Common Bean Genome. Perez de la Vega M, Santalla M, Marsolais F (eds). Series Compendium of Plant Genomes. Springer International Publishing, Cham, Switzerland. pp. 289-295. doi: 10.1007/978-3-319-63526-2_13

Activités professionnelles / intérêts

  • Membre du comité de rédaction, Legume Science
  • Rédacteur adjoint, Frontiers in Plant Science, section spécialisée d'amélioration des plantes
  • Membre du comité de rédaction, Editorial Board, Agronomy

Prix et études

  • B.Sc. en microbiologie, Université Laval, 1992
  • M.Sc. en biologie, Université Laval, 1995
  • Ph.D. en biologie, Université Concordia, 2001
  • Titulaire de bourse de recherche postdoctorale du CRSNG, 2001-2003

  • Distinguished Achievement Award (prix pour distinction honorifique), Bean Improvement Cooperative, 2019

Expérience et / ou de travail international

  • Associé de recherche, département de biologie, Université Duke, 2001-2002
  • Associé de recherche, département de biologie, Université de Caroline du Nord à Chapel Hill, 2002-2003

Principales publications

  1. Diapari M, Pandurangan S, Song Q, Cregan PB, Miklas P, Marsolais F (2016) High density SNP genotyping of lines with different storage protein composition reveals sites of interspecific introgression and markers associated with phaseolin or lectin deficiency. Annual Report of the Bean Improvement Cooperative 59, 1-2

    2016 - Consulter les détails de la publication

  2. De Ron, A.M., González, A.M., Papa, R., Bitocchi, E., Debouck, D.G., Brick, M.A., Fourie, D., Marsolais, F., Beaver, J.S., Geffroy, V., McClean, P.E., Lozano, R., Yuste-Lisbona, F., et Casquero, P.A. (2015). « Common bean. », dans De Ron, A.M. (dir.) - Grain Legumes, Series Handbook of Plant Breeding. Vol 10., Springer-Verlag GmbH, p. 1-36.

    2015 - Consulter les détails de la publication

  3. Nowak, J.J., Marsolais, F., et Tian, L.-N. (2015). « Establish in vitro regeneration technology in common bean (Phaseolus vulgaris) varieties for herbicide resistance development. », Biennial Bean Improvement Cooperative Meeting, Niagara Falls, Canada, November 2-3, 2015. (Affiche)

    2015 - Consulter les détails de la publication

  4. Pandurangan, S., Crosby, W.L., Pauls, K.P., et Marsolais, F. (2015). « Genomic analysis of polymorphisms conferring storage protein deficiency in common bean. », ASPB Midwestern Section’s Annual Meeting, Saint-Louis, MO, USA, March 21-22, 2015, Presentation. (Affiche)

    2015 - Consulter les détails de la publication

  5. Pandurangan, S., Crosby, W.L., Pauls, K.P., et Marsolais, F. (2015). « Genomic analysis of polymorphisms conferring storage protein deficiency in common bean. », EUCARPIA Symposium on Protein Crops, Pontevedra, Spain, May 4-7, 2015, Actas Asociacion Española Leguminosas, no. 6, 69-70.

    2015 - Consulter les détails de la publication

  6. Pandurangan, S., Crosby, W.L., Pauls, K.P., et Marsolais, F. (2015). « Genomic analysis of polymorphisms conferring storage protein deficiency in common bean. », EUCARPIA Symposium on Protein Crops, Pontevedra, Spain, May 4-7, 2015.

    2015 - Consulter les détails de la publication

  7. Pandurangan, S. et Marsolais, F. (2015). « Determining sulfur-limiting conditions for studies of seed composition in common bean (Phaseolus vulgaris). Molecular Physiology and Ecophysiology of Sulfur. », dans De Kok, LJ, Hawkesford, M.J., Rennenberg, H., Saito, K., and Schnug, E. (dir.) - Series Proceedings of the International Plant Sulfur Workshop, Springer, p. 207-213.

    2015 - Consulter les détails de la publication

  8. Bett, K.E., Stonehouse, R.G., Miklas, P.N., Dhaubhadel, S., Austin, R.S., et Marsolais, F. (2015). « Is the slow-darkening gene another allele at P? », Bean Improvement Cooperatives 2015, Niagara Falls, ON, Canada, November 1-4, 2015. (Résumé)

    2015 - Consulter les détails de la publication

  9. Ajewole, E., Marsolais, F., Jaskolski, M., et Pajak, A. (2015). « K+-Dependent and K+-independent asparaginase from common bean (Phaseolus vulgaris): mechanism of activation by K+. Oral presentation. », Eastern Regional Meeting of the Canadian Society of Plant Biologists, Toronto, Canada, November 20-21, 2015. (Présentation)

    2015 - Consulter les détails de la publication

  10. Joshi, J. et Marsolais, F. (2015). « Unraveling sulfur amino acid metabolism in common bean seeds (Phaseolus vulgaris). », 10th Jubilee Plant Sulfur Workshop, Goslar, Germany, September 1-4, 2015. (Affiche)

    2015 - Consulter les détails de la publication

  11. Kambhampati, S. et Marsolais, F. (2015). « Role of a class I glutamine amidotransferase (GAT1_2.1) in maintaining C/N balance in roots of Arabidopsis thaliana. », Botany 2015 Joint Conference: Science and plants for people, Shaw Convention Center, Edmonton, Alberta, Canada, July 25-29, 2015, Oral presentation.

    2015 - Consulter les détails de la publication

  12. Pandurangan, S., Diapari, M., Munholland, S., Perry, G.E., Crosby, W.L., Pauls, K.P., et Marsolais, F. (2015). « Genome wide analysis of beans with novel protein compositions. », Biennial Bean Improvement Cooperative Meeting, Niagara Falls, Canada, November 2-3, 2015, Invited oral presentation.

    2015 - Consulter les détails de la publication

  13. Shahmir, F.A., Marsolais, F., et Pauls, K.P. (2015). « Development and mapping of SNP markers in a resistant inter-cross population of common bean. », Biennial Bean Improvement Cooperative Meeting, Niagara Falls, Canada, November 2-3, 2015. (Affiche)

    2015 - Consulter les détails de la publication

  14. Ajewole, E., Pajak, A., Jaskolski, M., et Marsolais, F. (2015). « K+ Dependent and K+ independent asparaginase from common bean (Phaseolus vulgaris): mechanism of activation by K+. », 54th Annual Meeting of the Phytochemical Society of North America, Urbana-Champaign, August 8-12, 2015.

    2015 - Consulter les détails de la publication

  15. Cohen, R.D.H., Pajak, A., Pandurangan, S., Amir, R., et Marsolais, F. (2015). « Methionine over-accumulation in transgenic Arabidopsis seeds alters the composition of storage compounds. », 10th Jubilee Plant Sulfur Workshop, Goslar, Germany, September 1-4, 2015. (Affiche)

    2015 - Consulter les détails de la publication

  16. Pandurangan, S., Pajak, A., Rintoul, T.L., Beyaert, R.P., Hernández-Sebastià, C., Brown, D.C.W., et Marsolais, F. (2015). « Soybean seeds overexpressing asparaginase exhibit reduced protein concentration. », Physiologia Plantarum, 152(2), p. 126-137. doi : 10.1111/ppl.12341

    2015 - Consulter les détails de la publication

  17. Groves, R.A., Hagel, J.M., Zhang, Y., Kilpatrick, K.G., Levy, A., Marsolais, F., Lewinsohn, E., Sensen, C.W., et Facchini, P.J. (2015). « Transcriptome profiling of khat (Catha edulis) and Ephedra sinica reveals gene candidates potentially involved in amphetamine-type alkaloid biosynthesis. », PLoS ONE, 10(3: e0119701), p. 21 pages. doi : 10.1371/journal.pone.0119701

    2015 - Consulter les détails de la publication

  18. Pandurangan, S., Sandercock, M., Beyaert, R.P., Conn, K.L., Hou, A., et Marsolais, F. (2014). « Differential response to sulfur nutrition of two common bean genotypes differing in storage protein composition. », Frontiers in Plant Science, 6(Feb: Article 92), p. 1-11. doi : 10.3389/fpls.2015.00092

    2015 - Consulter les détails de la publication

  19. Pandurangan, S., Crosby, W.L., Pauls, K.P., et Marsolais, F. (2014). « Genomic analysis of mutations conferring storage protein deficiency in common bean. », Annual Report of the Bean Improvement Cooperative (BIC), 57(29).

    2014 - Consulter les détails de la publication

  20. Marsolais, F. (2014). « Protein Chemistry of grain legumes. », Science Day, Southern Crop Protection and Food Research Centre, London, ON, Canada, December 9, 2014. (Présentation)

    2014 - Consulter les détails de la publication

Contact

Installation de recherche

Centre de recherche et de développement de London
1391, rue Sandford
London, ON N5V 4T3
Canada

Expertise

Agriculture›Enzymologie
Agriculture›Génomique›Fonctionnelle
Agriculture›Légumineuses
Agriculture›Nutrition›Cultures
Agriculture›Protéomique
Plantes›Céréales et grain
Plantes›Oléagineux

Affiliations

  • Professeur de recherche auxiliaire (niveau 1), département de biologie, Université de Western Ontario
  • Membre du Sous-comité de la recherche, Ontario Pulse Committee
  • Membre, Bean Improvement Cooperative
  • Membre, AOCS, Division des protéines et co-produits
  • Membre, Société canadienne de biologie végétale

Langue

Anglais
Français