Frédéric Marsolais, Ph.D.

Image Frédéric Marsolais
Chercheur scientifique

Programme de recherche sur la chimie des protéines des légumineuses

Recherche et / ou projets en cours

Les travaux de recherches portent actuellement sur la chimie des protéines des légumineuses et le métabolisme des acides aminés dans les graines.

Énoncés de recherches/projets

  • Concevoir de nouveaux systèmes de fixation d'azote par les légumineuses et les rhizobiums pour réduire les émissions de protoxide d'azote des systèmes de culture
  • Métabolisme de l’asparagine et fonction de cet acide aminé dans la signalisation de l’azote

Publications récentes

Articles de recherche

  1. Kambhampati S, Hubbard A, Koley S, Gomez J, Marsolais F, Evans B, Young J (2024) SIMPEL: using stable isotopes to elucidate dynamics of context specific metabolism. Commun Biol 7, 172. doi: 10.1038/s42003-024-05844-z
  2. Bhowmik PK, Yan W, Hodgins C, Polley B, Warkentin T, Nickerson MT, Ro D-K, Marsolais F, Domoney C, Shariati-Ievari S, Aliani M (2023) CRISPR/Cas9-mediated lipoxygenase gene editing in yellow pea leads to major changes in fatty acid and flavor profiles. Front Plant Sci 14, 1246905. doi: 10.3389/fpls.2023.1246905
  3. Puri A, Pajak A, Hannoufa A, Marsolais F (2023) Quantitative proteomic analysis of the role of miRNA156 in alfalfa under drought stress. Environ Exp Bot 214, 105449. doi:10.1016/j.envexpbot.2023.105449
  4. Takahashi H, Marsolais F, Cuypers A, Kopriva S (2023) Sulfur metabolism : actions for plant resilience and environmental adaptation. J Exp Bot 74, 3271-3275. doi:10.1093/jxb/erad164 (Special issue: Sulfur metabolism: Actions for plant resilience and environmental adaptation, 12th International Plant Sulfur Workshop, London, Ontario, Canada, 2022)
  5. Pajak A, Zaman D, Ajewole E, Pandurangan S, Marsolais F. Diurnal accumulation of K+-dependent L-asparaginase in leaf of common bean (Phaseolus vulgaris L.) (2023) Phytochemistry 205, 113489. doi:10.1016/j.phytochem.2022.113489
  6. Xiong R, Wang A, Marsolais F, Todd C (2023) A fast and efficient method to introduce Apple Latent Spherical Virus to legume plants via Agrobacterium rhizogenes-mediated transformation of hairy roots. Legume Sci. 3, e154. doi:10.1002/leg3.154
  7. Fu Y-B, Cober ER, Morrison MJ, Marsolais F, Zhou R, Xu N, Gahagan AC, Horbach C (2022) Variability in maturity, oil and protein concentration, and genetic distinctness among soybean accessions conserved at Plant Gene Resources of Canada. Plants 11, 3525.
  8. Islam NS, Duwadi K, Chen L, Pajak A, McDowell T, Marsolais F, Dhaubhadel S (2022) Global analysis of common bean multidrug and toxic compound extrusion transporters (PvMATEs): PvMATE8 and pinto bean seed coat darkening. Front Plant Sci 13, 1046597. doi:10.3389/fpls.2022.1046597. Research topic “Specialized Metabolism in Legume Species - Defense and Medicinal Compounds”
  9. Palmer JP, Pajak A, Robson B, Zhang B, Joshi J, Diapari M, Pauls KP, Marsolais F (2022) PECTIN ACETYLESTERASE 8 influences pectin acetylation in the seed coat, seed imbibition and dormancy in common bean (Phaseolus vulgaris L.). Legume Sci 3, e130. doi:10.1002/leg3.130. Correction (2024) doi:10.1002/leg3.215
  10. Luo Y, Na R, Nowak J, Qiu Y, Lu QS, Yang C, Marsolais F, Tian L. (2021) Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV. BMC Plant Biol. 21, 419. doi:10.1186/s12870-021-03138-8
  11. Kambhampati S, Pajak A, Marsolais F (2021) Evidence that class I glutamine amidotransferase, GAT1_2.1, acts as a glutaminase in roots of Arabidopsis thaliana. Plant Sci 312, 111033. doi:10.1016/j.plantsci.2021.111033
  12. Fu Y-B, Cober ER, Morrison MJ, Marsolais F, Peterson GW, Horbach C (2021) Patterns of genetic variation in a soybean germplasm collection as characterized with genotyping-by-sequencing. Plants 10, 1611. doi: 10.3390/plants10081611. Special Issue on “Genetic Diversity, Conservation and Innovative Plant Breeding Strategies”
  13. Song J, Xie X, Chen C, Shu J, Thapa RK, Nguyen V, Bian S, Kohalmi SE, Marsolais F, Zou J, Cui Y (2021) Endosperm-synthesized pioneer-like transcription factor LEAFY COTYLEDON1 enables embryo maturation in Arabidopsis. Nat Commun 12, 3963. doi:10.1038/s41467-021-24234-1. Featured on Editors’ Highlights Focus webpage of “Plants and agriculture” in Nat Commun
  14. Viscarra-Torrico RC, Aga Pajak A, Soler Garzón A, Zhang B, Pandurangan S, Diapari M, Song Q, Conner RL, House JD, Miklas PN, Hou A, Marsolais F (2021) Common bean (Phaseolus vulgaris L.) with increased cysteine and methionine concentration. Legume Sci. e103. doi: 10.1002/leg3.103. Pre-harvest paper of the year
  15. Arshad M, Puri A, Simkovich AJ, Renaud J, Gruber MY, Marsolais F, Hannoufa A (2020) Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature. BMC Genomics 21, 758. doi: 10.1186/s12864-020-07161-1
  16. Reinprecht Y, Schram L, Marsolais F, Smith TH, Hill B and Pauls KP (2020) Effects of nitrogen application on nitrogen fixation in common bean production. Front. Plant Sci. doi: 10.3389/fpls.2020.01172.
  17. Islam N, Bett KE, Pauls KP, Marsolais F, Dhaubhadel S (2020) A basic helix-loop-helix transcription factor, PvTT8, encoded by the P gene, regulates postharvest seed coat darkening in pinto bean (Phaseolus vulgaris). Plants, People, Planet 2, 663-677. doi: 10.1002/ppp3.10132.
  18. Jiang Y, MacLean DE, Perry GE, Marsolais F, Hill B, Pauls KP (2020) Evaluation of beneficial and inhibitory effects of nitrate on nodulation and nitrogen fixation in common bean (Phaseolus vulgaris). Legume Sci. 2, e45. doi: 10.1002/leg3.45.
  19. Xiong R, Pajak A, Wang A, Yoshikawa N, Marsolais F, Todd CD (2019) Agrobacterium-mediated inoculation of asymptomatic Apple latent spherical virus as gene silencing vector in pea (Pisum sativum L.). Legume Sci. 1, e14. doi: 10.1002/leg3.14.  
  20. Wilker JL, Navabi A, Rajcan I, Marsolais F, Hill B, Torkamaneh D, Pauls PK (2019) Agronomic performance and nitrogen fixation of heirloom and conventional dry bean varieties under low-nitrogen field conditions. Front Plant Sci. 10, 952. doi: 10.3389/fpls.2019.00952. Research Topic: Legumes for Global Food Security
  21. Joshi J, Saboori-Robat E, Solouki M, Mohsenpour M, Marsolais F (2019) Distribution and possible biosynthetic pathway of non-protein sulfur amino acids in legumes. J Exp Bot 70, 4115-4121. doi: 10.1093/jxb/erz291. Special issue: Sulfur nutrition: impacts on plant development, metabolism, and stress responses.
  22. Joshi J, Renaud J, Sumarah M, Marsolais F (2019) Deciphering S-methylcysteine biosynthesis in common bean by isotopic tracking with mass spectrometry. Plant J 100, 176-186. doi: 10.1111/tpj.14438 (Cover figure)
  23. Chen C, Shu J, Li C, Thapa R, Nguyen V, Yu K, Yuan Z-C; Kohalmi S, Liu J, Marsolais F, Huang S, Cui Y (2019) RNA polymerase II independent recruitment of SPT6L at transcription start sites in Arabidopsis. Nucl Acids Res 47, 6714-6725. doi: 10.1093/nar/gkz465
  24. Saboori-Robat E, Joshi J, Pajak A, Solouki M, Mohsenpour M, Renaud J, Marsolais F. (2019) Common bean (Phaseolus vulgaris L.) accumulates most S-methylcysteine as its γ-glutamyl dipeptide. Plants 8, 126. doi: 10.3390/plants8050126. Special Issue "Advances in Plant Sulfur Research".

Chapitre de livre

  1. Wiesinger JA, Marsolais F, Glahn RP (2021) Health implications and nutrient bioavailability of bioactive compounds in dry beans and other pulses. In Dry Beans and Pulses Production, Processing and Nutrition, 2nd ed. Siddiq M, Uebersax MA, eds. Wiley. pp. 505-529. doi: 10.1002/9781119776802

Activités professionnelles / intérêts

  • Rédacteur adjoint, Legume Science
  • Rédacteur adjoint, Frontiers in Plant Science, section spécialisée d'amélioration des plantes

Prix et études

  • B.Sc. en microbiologie, Université Laval, 1992
  • M.Sc. en biologie, Université Laval, 1995
  • Ph.D. en biologie, Université Concordia, 2001
  • Titulaire de bourse de recherche postdoctorale du CRSNG, 2001-2003
  • Distinguished Achievement Award (prix pour distinction honorifique), Bean Improvement Cooperative, 2019

Expérience et / ou de travail international

  • Associé de recherche, département de biologie, Université Duke, 2001-2002
  • Associé de recherche, département de biologie, Université de Caroline du Nord à Chapel Hill, 2002-2003

Principales publications

  1. Wiesinger JA, Marsolais F, Glahn RP (2022) Health implications and nutrient bioavailability of bioactive compounds in dry beans and other pulses. In Dry Beans and Pulses Production, Processing and Nutrition, 2nd ed. Siddiq M, Uebersax MA, eds. Wiley. pp. 505-529. doi: 10.1002/9781119776802

    2021 - Consulter les détails de la publication

  2. Arshad, M., Puri, A., Simkovich, A.J., Renaud, J., Gruber, M.Y., Marsolais, F., Hannoufa, A. (2020). Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature. BMC Genomics, [online] 21(1), http://dx.doi.org/10.1186/s12864-020-07161-1

    2020 - Consulter les détails de la publication

  3. Islam, N.S., Bett, K.E., Pauls, K.P., Marsolais, F., Dhaubhadel, S. (2020). Postharvest seed coat darkening in pinto bean (Phaseolus vulgaris) is regulated by Psd, an allele of the basic helix-loop-helix transcription factor P. Plants People Planet, [online] 2(6), 663-677. http://dx.doi.org/10.1002/ppp3.10132

    2020 - Consulter les détails de la publication

  4. Marsolais F. (2019) Soybean Research Initiatives. Oral presentation. Soy Canada Annual General Meeting. London, June 18, 2019.

    2020 - Consulter les détails de la publication

  5. Marsolais F, Hou A, Conner RL, Pajak A (2019) Development of edible bean germplasm lines with improved cysteine and methionine concentration. Poster presentation. 2019 Biennial Bean Improvement Cooperative Meeting. Fargo, ND, November 3-6

    2019 - Consulter les détails de la publication

  6. Reinprecht Y, Schram L, Qiu T, Baerg L, Marsolais F, Smith T, Pauls KP (2019) Effects of nitrogen application on nitrogen fixation in common bean production. 2019 Biennial Bean Improvement Cooperative Meeting. Fargo, ND, November 3-6

    2019 - Consulter les détails de la publication

  7. Joshi, J., Renaud, J.B., Sumarah, M.W., Marsolais, F. (2019). Deciphering S-methylcysteine biosynthesis in common bean by isotopic tracking with mass spectrometry. Plant Journal, [online] 100(1), 176-186. http://dx.doi.org/10.1111/tpj.14438

    2019 - Consulter les détails de la publication

  8. Wilker JL, Navabi A, Rajcan I, Marsolais F, Hill B, Torkamaneh D, Pauls PK (2019) Agronomic performance and nitrogen fixation of heirloom and conventional dry bean varieties under low-nitrogen field conditions. Oral presentation. Plant Canada. University of Guelph. July 7-10.

    2019 - Consulter les détails de la publication

  9. Marsolais F (2018) Improvement of sulfur amino acid concentration in common bean. Invited full talk. Sulfur in agriculture and ecophysiology session. 11th International Plant Sulfur Workshop. Conegliano, Italy, September 16-20

    2018 - Consulter les détails de la publication

  10. Reinprecht Y, Schram L, Qiu T, Baerg L, Marsolais F, Smith TH, KP Pauls (2018) Effect of cultivar mixtures on yield in common bean. Poster presentation. National Association of Plant Breeders Annual Meeting. Guelph, ON, August 7-10

    2018 - Consulter les détails de la publication

  11. Islam NS, Marsolais F, Dhaubhadel S (2018) Arabidopsis TT8 orthologue of pinto bean (Phaseolus vulgaris L.) regulates proanthocyanidin genes and seed coat darkening. Plant Biology 2018, Montreal, July 13-18

    2018 - Consulter les détails de la publication

  12. Joshi J, Renaud JB, Marsolais F (2018) Biosynthesis of S-methylcysteine in developing seeds of common bean as studied by isotopic tracking and high resolution mass spectrometry. Poster presentation. Plant Biology 2018, Montreal, July 13-18

    2018 - Consulter les détails de la publication

  13. Puri A, Hannoufa A, Marsolais F (2018) Quantitative proteome analysis of alfalfa in drought stress under the influence of miR156 (Medicago sativa). North American Alfalfa Improvement Conference. Logan, Utah, June 4-6

    2018 - Consulter les détails de la publication

  14. Duwadi, K., Austin, R.S., Mainali, H.R., Bett, K., Marsolais, F., Dhaubhadel, S. (2018). Slow darkening of pinto bean seed coat is associated with significant metabolite and transcript differences related to proanthocyanidin biosynthesis. BMC Genomics, [online] 19(1), http://dx.doi.org/10.1186/s12864-018-4550-z

    2018 - Consulter les détails de la publication

  15. Joshi J, Pandurangan S, Diapari M, Marsolais F. Comparison of gene families: seed storage proteins. In The Common Bean Genome. Perez de la Vega M, Santalla M, Marsolais F (eds). Series Compendium of Plant Genomes. Springer International Publishing, Cham, Switzerland. pp. 201-217. doi: 10.1007/978-3-319-63526-2_10

    2017 - Consulter les détails de la publication

  16. Palmer J, Pajak A, Marsolais F (2017) Identification and characterization of a pectin acetylesterase from the seed coat of common bean (Phaseolus vulgaris). Oral presentation. 2017 Eastern Regional Meeting of the Canadian Society of Plant Biologists. Sainte-Anne-de-Bellevue, QC, November 25, 2017

    2017 - Consulter les détails de la publication

  17. Palmer J, Pajak A, Marsolais F (2017) Identification and characterization of a pectin acetylesterase from the seed coat of common bean (Phaseolus vulgaris). Oral presentation. 2017 Biennial Bean Improvement Cooperative Meeting. East Lansing, MI, October 29-November 1

    2017 - Consulter les détails de la publication

  18. Islam, N., Marsolais, F. and Dhaubhadel, S. (2017) Identification and characterization of slow darkening gene in pinto bean (Phaseolus vulgaris L.) Biennial Bean Improvement Cooperative Meeting, East Lansing, MI, Oct 29-31

    2017 - Consulter les détails de la publication

  19. Pajak A, Santamaria-Kisiel L, Marsolais F (2016) Mapping of a pepsin-resistant peptide and identification of glycosylation sites in the α subunit of the 11S globulin legumin from common bean. Oral presentation. Tenth Canadian Pulse Research Workshop, Winnipeg , October 25-28

    2017 - Consulter les détails de la publication

  20. Chen C, Li C, Wang Y, Renaud J, Tian G, Kambhampati S, Saatian B, Nguyen V, Hannoufa A, Marsolais F, Yuan ZC, Yu K, Austin RS, Liu J, Kohalmi SE, Wu K6, Huang S, Cui Y (2017). "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis". Nature Plants. 3(10):814-824. doi: 10.1038/s41477-017-0023-7. Epub 2017 Sep 25.

    2017 - Consulter les détails de la publication

Installation de recherche

1391, rue Sandford
London, ON N5V 4T3
Canada

Affiliations

  • Professeur de recherche auxiliaire (niveau 1), département de biologie, Université de Western Ontario
  • Membre, Ontario Pulse Crop Committee
  • Membre, Société Phytochimique de l'Amérique du Nord

Langue

Anglais
Français