Dr Jonathan Griffiths
Virologue moléculaire végétal
Recherche et / ou projets en cours
Plant viruses are serious pathogens that can be difficult to study. In my lab, we are using multiple different approaches towards understanding and combating plant viral pathogens. Plum pox virus has been a major issue for stone fruit crops in Ontario for years. We are currently evaluating novel approaches to reduce the impact of this virus including a non-transgenic, topical RNAi approach to either downregulate host genes required for viral replication, or directly targeting this virus to reduce replication and viral load. We are also heavily involved in investigating the role of viruses in Sudden Apple Decline and Tree fruit decline, two disorders that have recently been plaguing tree fruit producers across Canada. We are using traditional surveying and pathology approaches to understand the distribution of major plant viruses in tree fruits. In grapes, we are employing an omics-based approach to understand the distribution and variation of major grape viruses in Ontario, which will be followed up with a reverse genetics approach to understand the function of major viral proteins. We are developing a novel survey approach using commercial pollination services to obtain quicker and cheaper images of plant virus distribution. We are evaluating how to sample honey bees used to pollinate fruit trees to detect plant viruses using an RNAseq approach. Finally, we are actively studying Tomato brown rugose fruit virus and Pepino mosaic virus, two similarly transmitted, yet distantly related tomato pathogens. We are also using a genetic mapping/positional cloning approach to identify sources of resistance to Tomato brown rugose fruit virus.
Énoncés de recherches/projets
Current projects:
-Plant virus surveillance using bees
-Investigating the cause of tree fruit decline
-Development of resistance to Plum pox virus in peach
-Development of resistance to Tomato brown rugose fruit virus
-Genomics of grapevine viruses
Prix et études
Ph.D. UBC Botany, 2013
AgreenSkills post-doctoral Fellowship, Marie Curie Co-fund, 2015-2017
Liens additionnels
Principales publications
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Ellouze, W., Griffiths, J. (2021) AAFC research update on sudden apple decline and fruit tree decline disorders in Ontario. Orchard Network Newsletter, February 2021. p. 16-19.
2021 - Consulter les détails de la publication
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Burlakoti R. and Griffiths J. 2020. Tomato Brown Rugose Fruit Virus research project. BC Greenhouse Growers Association Town Hall Minor Use Meeting, Virtual. November 5, 2020.
2020 - Consulter les détails de la publication
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Griffiths, J., Ellouze, W. (2019) Research activities working towards identifying causes of tree fruit decline. Orchard Network Newsletter, December 2019. p. 6-7.
2019 - Consulter les détails de la publication
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Nguyen HDT, Grunwald NJ, Abad ZG, Martin FN, Crouch J, Hambleton S, Dettman J, Liu M, Harris LJ, Castlebury L, Salagado-Salazar C, Cowger C, Ellouz O, Mollov D, Fall ML, Ling K, Griffifths J, Mahaffee W, Chen W, Sultata T, Yu Q, Carta LK, Skantar AM, Tambong J, Rogers E, Li S, Dumonceaux TJ, Widmer TL. The 2019 AAFC-USDA meeting on genotyping and monitoring of high-risk plant pathogens.
2019 - Consulter les détails de la publication
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Griffiths, J., Lofano, A., Ellouze, W., Wang, A. (2019) Apple and apricot decline in Ontario. Plant Canada 2019, July 7-10. University of Guelph, Guelph, Ontario, Canada.
2019 - Consulter les détails de la publication
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Babu, M.R., Griffiths, J.S., et Wang, A.M. (2010). « Isolation and transfection of plant protoplasts for studying infection and replication of plant viruses. », dans Wang, A. (dir.) - Principles and Practice of Advanced Technology in Plant Virology, Research Signpost, Kerala, India, Chapitre 8, p. 141-152.
2010 - Consulter les détails de la publication
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Griffiths, J.S. et Wang, A.M. (2007). « Isolation and Characterization of Arabidopsis Translation Initiation Factors Associated with Plum Pox Virus Infection. », Plant Biology 2007 and Botany 2007 Joint Congress, Hilton Chicago, Chicago, IL, USA, July 7-11, 2007.
2007 - Consulter les détails de la publication
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Wang, A.M., Babu, M.R., Huang, T.-S., Griffiths, J.S., Yi, J., Gagarinova, A.G., et Chen, L. (2007). « Virus-plant interactions: molecular identification of host genes associated with potyvirus infection. », Plant Biology 2007 and Botany 2007 Joint Congress, Hilton Chicago, Chicago, IL, USA, July 7-11, 2007.
2007 - Consulter les détails de la publication
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Raghupathy, M.B., Griffiths, J.S., Stobbs, L.W., Brown, D.C.W., Brandle, J.E., et Wang, A.M. (2006). « Transfection of Arabidopsis protoplast with a Plum pox virus (PPV) infectious clone for studying early molecular events associated with PPV infection. », Journal of Virological Methods, 136(1-2), p. 147-153.
2006 - Consulter les détails de la publication
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Griffiths, J.S., Babu, M.R., et Wang, A.M. (2006). « Analysis of Arabidopsis translation factors in Plum pox virus infection. », 25th Annual Meeting of the American Society for Virology, University of Wisconsin-Madison, Madison, WI, USA, July 15-19, 2006.
2006 - Consulter les détails de la publication
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Raghupathy, M.B., Griffiths, J.S., Stobbs, L.W., Brown, D.C.W., Brandle, J.E., et Wang, A.M. (2006). « A genome-wide microarray study in Arabidopsis: activation and suppression of gene expression in response to plum pox virus infection. », 25th Annual Meeting of the American Society for Virology, University of Wisconsin-Madison, Madison, WI, USA, July 15-19, 2006.
2006 - Consulter les détails de la publication
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Wang, A.M., Raghupathy, M.B., Griffiths, J.S., Chen, L., et Huang, T.-S. (2005). « Understanding of molecular PPV-host interactions and development of novel strategies against PPV. », 2005 Annual meeting, NE1006: Eradication, Containment and/or Management of Plum Pox Disease (Sharka), Niagara Falls, ON, Canada, October 25-27, 2005.
2005 - Consulter les détails de la publication
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Raghupathy, M.B., Griffiths, J.S., Chen, L., Stobbs, L.W., Brown, D.C.W., Brandle, J.E., et Wang, A.M. (2005). « Isolation of Arabidopsis protoplasts for studying gene expression in response to Plum pox virus infection. », 2005 Annual meeting, NE1006: Eradication, Containment and/or Management of Plum Pox Disease (Sharka), Niagara Falls, ON, Canada, October 25-27, 2005.
2005 - Consulter les détails de la publication
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Wu, K., Griffiths, J.S., et McKellar, R.C. (2000). « A comparison of the Bioscreen method and microscopy for the determination of lag times of individual cells of Listeria monocytogenes. », Letters in Applied Microbiology, 30(6), p. 468-472.
2000 - Consulter les détails de la publication
Contact
Installation de recherche
Affiliations
Adjunct Professor, Brock University
Associate Editor, Canadian Journal of Plant Pathology