Brad S. Pickering, PhD

Image Brad Pickering
Chercheur scientifique (Unité des agents pathogènes spéciaux)

L’étude des virus zoonotiques d’importance vétérinaire, y compris la pathogenèse, l’interaction hôte-virus, la transmission, les modèles animaux et la mise au point de tests diagnostiques.

Recherche et / ou projets en cours

  • Mes travaux de recherche actuels portent sur les agents zoonotiques à conséquences élevées d’importance vétérinaire, y compris les hénipavirus, les ébolavirus et le virus de la fièvre de la vallée du Rift.

Énoncés de recherches/projets

  • Essais d’efficacité du vaccin contre le virus Nipah et corrélats de protection chez les porcs.
  • Développement du diagnostic du virus Ebola en utilisant des applications de sérologie et de biologie moléculaire.
  • La co-infection et ses effets sur la progression de la maladie de l’ébolavirus chez les porcs.

Prix et études

  • B.Sc. avec distinction, Microbiologie, Université du Manitoba, 2003
  • Doctorat en microbiologie, Université du Manitoba, 2010
  • Boursier postdoctoral, Children's Hospital Boston et Harvard Medical School, 2009-2012
  • Bourse de recherche scientifique du Conseil de recherches en sciences naturelles et en génie (CRSNG), ACIA, Unité des agents pathogènes spéciaux du CNMAE, 2012-2013
  • Boursier postdoctoral, Université du Manitoba, Unité des agents pathogènes spéciaux du CNMAE, 2013-2014

Principales publications

Hongzhao Li, Alexander Bello, Greg Smith, Dominic M S Kielich, James E Strong, and Bradley S Pickering. (2022). Degenerate sequence-based CRISPR diagnostic for Crimean–Congo hemorrhagic fever virus. PLOS Neglected tropical diseases. 16(3) http://dx.doi.org/10.1371/journal.pntd.0010285.

 

Chandrika Senthilkumaran, Andrea Kroeker, Gregory Smith, Carissa Embury-Hyatt, Bradley Collignon, Elizabeth Ramirez-Medina, Paul A. Azzinaro, Bradley Pickering, Fayna Diaz-San Segundo, Hana M. Weingartl, Teresa de los Santos. (2022). Treatment with Ad5-porcine interferon-αattenuates Ebolavirus disease in pigs. Pathogens. 11: 449. http://dx.doi.org/doi.org/10.3390/pathogens11040449

Amalfitano E, Karlikow M, Norouzi M, Jaenes K, Cicek S, Masum F, Sadat Mousavi P, Guo Y, Tang L, Sydor A, Ma D, Pearson JD, Trcka D, Pinette M, Ambagala A, Babiuk S, Pickering B, Wrana J, Bremner R, Mazzulli T, Sinton D, Brumell JH, Green AA, Pardee K. (2021). A glucose meter interface for point-of-care gene circuit-based diagnostics. Nat Commun.12(1): 724.

 

Christine Atherstone, Sandra Diederich, Bradley Pickering, Greg Smith, Graham Casey, Kerstin Fischer, Michael P. Ward, Dickson Ndoboli, Hana Weingartl, Silvia Alonso, Navneet Dhand, Kristina Roesel, Delia Grace, Siobhan M. Mor. (2021). Investigation of Ebolavirus exposure in pigs presented for slaughter in Uganda. Transbound. Emerg. Dis.68(3): 1521-1530.

 

Pickering BS, Smith G, Pinette MM, Embury-Hyatt C, Moffat E, Marszal P, Lewis CE. (2021). Susceptibility of Domestic Swine to Experimental Infection with Severe Acute Respiratory Syndrome Coronavirus 2. Emerging Infectious diseases. 1: 104-112.

 

Biondi MJ, Garnett L, Bello A, Funk D, Poliquin PG, Jones S, Tierney K, Tran K, Kozak RA, Leung A, Grolla A, Nakamura C, Soule G, Ranadheera C, Hagan M, Dhaliwal A, Kobasa D, Falzarano D, Bovendo HF, Feldmann H, Kesselman M, Hansen G, Gren J, Mortimer T, Racine T, Deschambault Y, Edmonds J, Aminian S, Saurette R, Allan M, Rondeau L, Huynh J, Hadder S, Press C, DeGraff C, Kucas S, Kubay J, Azanarsky K, Cook BWM, Hancock BJ, Kumar A, Soni R, Schantz D, McKitrick J, Warner B, Griffin BD, Qiu X, Kobinger GP, Safronetz D, Wood H, R Stein D, Cutts T, Pickering B, Kenny J, Theriault S, Menec L, Vendramelli R, Higgins S, Banadyga L, Liu G, Rahim MN, Kasloff S, Sloan A, He S, Tailor N, Albietz A, Wong G, Gray M, Feldmann F, Marzi A, Risi G, Strong JE. (2021). Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment. Microorganisms. 9(3): 498.

Berhane Y, Suderman M, Babiuk S, Pickering B. (2021). Susceptibility of turkeys, chickens and chicken embryos to SARS-CoV-2. Transboundary and emerging disease. 68(6): 3038-3042. http://dx.doi.org/10.1111/tbed.13970

 

Chukwunonso Onyilagha, Henna Mistry, Peter Marszal, Mathieu Pinette, Darwyn Kobasa, Nikesh Tailor, Yohannes Berhane, Charles Nfon, Bradley Pickering, Samira Mubareka, David Bulir, Sylvia Chong, Robert Kozak & Aruna Ambagala. (2021). Evaluation of mobile real-time polymerase chain reaction tests for the detection of severe acute respiratory syndrome coronavirus 2.Sci Rep. 11: 9387.

Brad Pickering, Charles E Lewis. (2021). Balancing Animal Welfare, Human Safety, and Research in Agriculture High Containment. ILAR. 61(1): 1-2.

 

Ambagala A, Schwantje H, Laurendeau S, Snyman H, Joseph T, Pickering B, Hooper-McGrevy K, Babiuk S, Moffat E, Lamboo L, Lung O, Goolia M, Pinette M, Embury-Hyatt C. (2021). Incursions of rabbit haemorrhagic disease virus 2 in Canada-Clinical, molecular and epidemiological investigation. Transbound Emerg Dis.68(4): 1711-1720.

 

Biondi MJ, Garnett L, Bello A, Funk D, Poliquin PG, Jones S, Tierney K, Tran K, Kozak RA, Leung A, Grolla A, Nakamura C, Soule G, Ranadheera C, Hagan M, Dhaliwal A, Kobasa D, Falzarano D, Bovendo HF, Feldmann H, Kesselman M, Hansen G, Gren J, Mortimer T, Racine T, Deschambault Y, Edmonds J, Aminian S, Saurette R, Allan M, Rondeau L, Huynh J, Hadder S, Press C, DeGraff C, Kucas S, Kubay J, Azanarsky K, Cook BWM, Hancock BJ, Kumar A, Soni R, Schantz D, McKitrick J, Warner B, Griffin BD, Qiu X, Kobinger GP, Safronetz D, Wood H, R Stein D, Cutts T, Pickering B, Kenny J, Theriault S, Menec L, Vendramelli R, Higgins S, Banadyga L, Liu G, Rahim MN, Kasloff S, Sloan A, He S, Tailor N, Albietz A, Wong G, Gray M, Feldmann F, Marzi A, Risi G, Strong JE. (2021). Characterization of Ebola Virus Risk to Bedside Providers in an Intensive Care Environment. Microorganisms. 26(3): 498.

 

Chan M, Leung A, Hisanaga T, Pickering B., Griffin B., Vendramelli R., Tailor N., Wong G., Bi Y., Babiuk S., Berhane Y., and Darwyn Kobasa. (2020). H7N9 Influenza VirusContaining a Polybasic HA Cleavage Site Requires Minimal Host Adaptation to Obtain a Highly Pathogenic Disease Phenotype in Mice. Viruses.12(65): 10.3390/v12010065.

 

Charles E Lewis, Bradley Pickering. (2020). Livestock and Risk Group 4 Pathogens: Researching Zoonotic Threats to Public Health and Agriculture in Maximum Containment. ILAR Journal. 61(1): 86-102.

 

Kristian M Forbes, Omu Anzala, Colin J Carlson, Alyson A Kelvin, Krutika Kuppalli, Eric M Leroy, Gael D Maganga, Moses M Masika, Illich M Mombo, Dufton M Mwaengo, Roch F Niama, Julius Nziza, Joseph Ogola, Brad S Pickering, Angela L Rasmussen, Tarja Sironen, Olli Vapalahti, Paul W Webala, Jason Kindrachuk. (2020). Towards a coordinated strategy for intercepting human disease emergence in Africa. Lancet Microbe 2020. 2: e51-e52.

 

Kroeker AL, Smid V, Embury-Hyatt C, Collignon B, Pinette M, Babiuk S and Pickering B. (2020). Increased Susceptibility of Cattle to Intranasal RVFV Infection. Frontiers in Veterinary Science. 7(137): 1-11.

 

Poliquin G, Funk D, Jones S, Tran K, Ranadheera C, Hagan M, Tierney K, Grolla A, Dhaliwal A, Bello A, Leung A, Nakamura C, Kobasa D, Falzarano D, Garnett L, Bovendo HF, Feldmann H, Kesselman M, Hansen G, Gren J, Risi G, Biondi M, Mortimer T, Racine T, Deschambault Y, Aminian S, Edmonds J, Saurette R, Allan M, Rondeau L, Hadder S, Press C, DeGraff C, Kucas S, Cook BWM, Hancock BJ, Kumar A, Soni R, Schantz D, McKitrick J, Warner B, Griffin BD, Qiu X, Kobinger GP, Safronetz D, Stein D, Cutts T, Kenny J, Soule G, Kozak R, Theriault S, Menec L, Vendramelli R, Higgins S, Banadyga L, Liu G, Rahim MN, Kasloff S, Sloan A, He S, Tailor N, Albietz A, Pickering B, Wong G, Gray M, Strong JE. (2019). Impact of intensive care unit supportive care on thephysiology of Ebola virus disease in a universally lethal nonhuman primatemodel. Intensive Care Medicine Experimental. 7(66): 1-13.

 

Kasloff SB, Leung A, Pickering B, Moffat E, Embury-Hyatt C, Kobasa D, Weingartl HM. (2019). Pathogenicityof Nipah henipavirus Bangladesh in a swine host.Scientific Reports. 9(5230): 1.

Poliquin G., Funk D., Jones S., et al. (2019). Impact of intensive care unit supportive care on the physiology of Ebola virus disease in a universally lethal non-human primate model. Intensive Care Med Exp.7: 54.

Brad S Pickering, Jessica R Spengler, Elnaz Shadabi, Antonia E Dalziel, Elizabeth A Lautner, Primal Silva. (2019). The Biosafety Level 4 Zoonotic Laboratory Network (BSL4ZNet): Report of a workshop on Live animal Handling. Antiviral Research. 172: 104640.

Vijayakumares V, Vanhove A, Pickering B, Szu Yu.L, Tierney B, jasara, Bronson R, Watnick P. (2018). Removal of a membraneanchor reveals the opposing regulatory functions of Vibrio choleraeglucosespecific enzyme llA. mBio. 1: 1.

Liao J, Gibson JA, Pickering BS, and Watnick PI. (2018). Sublingual adjuvant delivery by a live attenuated Vibrio cholerae-based antigen presentation platform. mSphere. 3: 1.

Xu W, Hole K, Goolia M, Pickering B, Salo T, Lung O, et al. (2017) Genome wide analysis of the evolution of Senecavirus A from swine clinical material and assembly yard environmental samples. PLoS ONE 12(5): e0176964

B.S. Pickering, B. Collignon, G. Smith, P. Marszal, G. Kobinger, and H.M. Weingartl, 2017. Detection of ZEBOV in swine: Assay development and optimization. Transbound Emerg Dis. 2017 Mar 27. doi: 10.1111

Brad S. Pickering, John M. Hardham, Greg Smith, Eva Weingartl, Paul Dominowski, Dennis Foss, Duncan Mwangi, Christopher C. Broder, James A. Roth, Hana M. Weingartl, 2016. Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response. Vaccine. 14;34(40):4777-86. Doi: 10.1016/j.vaccine.2016.08.028.

Yohannes Berhane, Darwyn Kobasa, Carissa Embury-Hyatt, Brad Pickering, Shawn Babiuk, et al., 2015. Molecular and Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolate from British Columbia, 2015. Sci Rep. 2016; 6: 23380.

Leng Y., Vakulskas CA., Zere TR., Pickering BS., Watnick PI., Babitzke P., Romeo T., 2015. Regulation of CsrB/C sRNA decay by EIIAGlc of the phosphoenolpyruvate: carbohydrate phosphotransferase system. Molecular Microbiology. DOI: 10.1111/mmi.13259.

Pickering BS, Tyler S, Smith G, Burton L, Li M, Dallaire A, et al. 2015. Identification of a Novel Afipia Species Isolated from an Indian Flying Fox. PLoS ONE 10(4): e0121274. doi:10.1371/journal.pone.0121274

Pickering, B., Lopilato J., Smith D.R., and Watnick P.I., 2014. The Transcription Factor Mlc Promotes Vibrio cholerae Biofilm Formation through Repression of Phosphotransferase System Components. Journal of Bacteriology, 196(13): 2423-2430.

Pickering B.S., Smith D.R., and Watnick P.I., 2012. Glucose-Specific Enzyme IIA Has Unique Binding Partners in the Vibrio cholerae Biofilm. MBio, e00228-12.

Pickering, B.S., Yudistira H and Oresnik, I.J., 2012. Characterization of the Twin-Arginine Transport Secretome in Sinorhizobium meliloti and Evidence for Host-Dependent Phenotypes. Applied and Environmental Microbiology, 79(19): 7141-7144.

Houot L, Chang S, Pickering BS, Absalon C., Watnick, PI. 2010. The phosphoenolpyruvate phosphotransferase system regulates Vibrio Cholerae biofilm formation through multiple independent pathways. Journal of Bacteriology, 192(16):4083-5.

Pickering, B.S., and Oresnik, I.J., 2010. The Twin Arginine Transport System Appears To Be Essential for Viability in Sinorhizobium meliloti. Journal of Bacteriology, 192(19):5173-80

Barney A. Geddes., Brad S. Pickering., Nathan J. Poysti, Heather Collins, Harry Yudistira and Ivan J. Oresnik, 2010. A Locus Necessary for the Transport and Catabolism of erythritol in Sinorhizobium meliloti. Microbiology, Oct;156 (Pt 10): 2970-81.

Pickering, B.S. and Oresnik, I.J., 2008. Formate-dependent autotrophic growth in Sinorhizobium meliloti. Journal of Bacteriology, 190(19): 6409-18.

Installation de recherche

1015, rue Arlington
Winnipeg, MB R3E 3M4
Canada

Affiliations

Université du Manitoba, département de microbiologie médicale et des maladies infectieuses

Langue

Anglais