Abdelali (Ali) Hannoufa, Ph.D.

Image Mathieu Rioux
Chercheur Scientifique

Chercheur scientifique et professeur adjoint en génomique et biotechnologie

Recherche et / ou projets en cours

  1. Recherche scientifique pour améliorer la luzerne
  2. Tolérance de la luzerne au stress
  3. Plants-pièges selon le principe de l’interférence ARN en horticulture
  4. Nouvelles méthodes biologiques pour atténuer les stress abiotiques chez les légumineuses fourragères et réduire les émissions de méthane des ruminants
  5. Mise au point d’outils de lutte intégrée contre les insectes nuisibles dans les serres au moyen d’approches génomiques et écologiques
  6. Amélioration de la productivité et de la persistance de la luzerne dans des conditions météorologiques extrêmes

Prix et études

Ph. D. en biologie moléculaire des plantes, York University, 1996

M. Sc. en biochimie des plantes, Concordian University, 1991

Principales publications

  1. Subedi U, Burton Hughes K, Chen G, Hannoufa A, Singer SD (2022) Eliciting targeted mutations in Medicago sativa using CRISPR/Cas9-mediated genome editing: A potential tool for the improvement of disease resistance. In: Plant-Pathogen Interactions (Foroud N, Neilson J, eds). Springer, NY.

    2022 - View publication details

  2. Gamalat Allam, Yousef Papadopoulos, Mark A. Bernards, Abdelali Hannoufa (2022) miR156/SPL network negatively regulates aluminum stress tolerance in Medicago sativa by targeting SPL13. 2022 Meeting of the Canadian Association for Plant Biotechnology, May 22-25, Quebec City, Quebec (oral presentation)

    2022 - View publication details

  3. Vida Nasrollahi, Susanne E. Kohalmi, Abdelali Hannoufa (2022) SPL12 regulates nodulation under osmotic stress and nitrate sufficiency conditions by targeting AGL6 and AGL21 in Medicago sativa. 2022 Meeting of the Canadian Association for Plant Biotechnology, Quebec City, Quebec 2022/08/22 - 2022/08/25 (oral presentation).

    2022 - View publication details

  4. Feyissa, B.A., Amyot, L., Nasrollahi, V., Papadopoulos, Y., Kohalmi, S.E., Hannoufa, A. (2021). Involvement of the miR156/SPL module in flooding response in Medicago sativa, 11(1), http://dx.doi.org/10.1038/s41598-021-82450-7

    2021 - View publication details

  5. Dan-Dobre M, Karagiannis J, Hannoufa A (2021) Characterization of the role of SPL4 in the regulation of drought stress and trichome development in alfalfa. The Eastern Regional Meeting of the Canadian Society of Plant Biologists. Careleton University, Nov 27 (virtual)

    2021 - View publication details

  6. Nasrollahi V, Kohalmi SE, Hannoufa A (2021) Characterization of SPL12 role in regulating root architecture, nodulation and nitrogen fixation in Medicago sativa. The Eastern Regional Meeting of the Canadian Society of Plant Biologists. Carleton University, Nov 27 (virtual)

    2021 - View publication details

  7. Dan-Dobre M, Karagiannis J, Hannoufa A (2021) Characterization of the role of SPL4 in trichome development and drought response in Medicago sativa. 11th Biennial Biology Graduate Research Forum, University of Western Ontario, Nov 5, 2021

    2021 - View publication details

  8. Nasrollahi V, Kohalmi SE, Hannoufa A (2021) miR156-SPL12-AGL6 module regulates root architecture and nodulation in Medicago sativa (alfalfa). 11th Biennial Biology Graduate Research Forum, University of Western Ontario, Nov 5, 2021

    2021 - View publication details

  9. Nasrollahi V., Kohalmi S., Hannoufa A. (2021) miR156/SPL12 modulates nodulation, nitrogen fixation and root regeneration in Medicago sativa by Silencing AGAMOUS-LIKE 6”. European Nitrogen Fixation Conference, Aarhus University, Denmark , October 8, 2021 (Virtual)

    2021 - View publication details

  10. Allam G, Papadopoulos Y, Bernards BA, Hannoufa A (2021) miR156/SPL network negatively regulates aluminum stress tolerance in Medicago sativa. The 2021 Annual General Meeting of the Canadian Society of Plant Physiologists (virtual), Saskatoon, SK, June 7-10, 2021

    2021 - View publication details

  11. Nasrollahi V, Kohalmi SE, Hannoufa A (2021) Characterization of SPL12 role in regulating root architecture, nodulation and nitrogen fixation in Medicago sativa (2021) The 2021 Annual General Meeting of the Canadian Society of Plant Physiologists (virtual), Saskatoon, SK, June 7-10, 2021

    2021 - View publication details

  12. Dan-Dobre M, Karagiannis J, Hannoufa A (2021) Characterization of SPL4 role in drought stress and trichome development in alfalfa. The 2021 Annual General Meeting of the Canadian Society of Plant Physiologists (virtual), Saskatoon, SK, June 7-10, 2021

    2021 - View publication details

  13. Nasrollahi V., Kohalmi S., Hannoufa A. (2021) Role of miR156/SPL in rot architecture and nodulation in Medicago sativa (alfalfa). Webinar on Plant Science and Genomics 2021 (WPSG 2021), April 29-30, 2021

    2021 - View publication details

  14. The plant-specific transcription factors Squamosa Promoter Binding Protein-Like (SPL) are involved in a range of vital biological and regulatory processes, such as vegetative growth, flowering and stress response. While the SPL gene family has been well studied in some plants, a systematic study has not yet been reported in alfalfa (Medicago sativa). In the present study, a total of 28 novel SPL members, including six alfalfa-specific ones, were identified using in silico C-based analysis of the recently released genome sequence, and named based on their closest orthologs in Medicago truncatula. The SPL-specific SBP domain was detected in all members, and 16 of the 28 members showed miR156 recognition target sequences. Maximum-Likelihood phylogenetic analyses showed the presence of five subclades and three outgroup members. The sequences within groups showed motif conservation, which may imply the presence of redundant functionality between certain SPL members. However, the members overall had divergent motifs indicating diverse functional roles of SPLs in alfalfa. A large number of redundant and/or highly similar sequences observed during synteny analyses revealed extensive gene duplication within the alfalfa genome. This might imply that MsSPL gene expansion stemmed from segmental duplication processes. The present study provides a comprehensive foundation for elucidating the biological functions of these transcription factors and thereby provides new tools for biotechnological improvement of important alfalfa attributes.

    2021 - View publication details

  15. Lei, Y., Hannoufa, A., Yu, P. (2020). Overexpression of miR156 and Silencing SPL6RNAi and SPL13RNAi Genes in Medicago sativa on the Changes of Carbohydrate Physiochemical, Fermentation, and Nutritional Profiles. Journal of Agricultural and Food Chemistry, [online] 68(49), 14540-14548. http://dx.doi.org/10.1021/acs.jafc.0c02508

    2020 - View publication details

  16. Arshad, M., Puri, A., Simkovich, A.J., Renaud, J., Gruber, M.Y., Marsolais, F., Hannoufa, A. (2020). Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature, 21(1), http://dx.doi.org/10.1186/s12864-020-07161-1

    2020 - View publication details

  17. Subedi U, Burton Hughes K, Dhariwal G, Kader K, Hannoufa A, Acharya S, Singer SD. Mutation of MsSPL8 alleles via CRISPR/Cas9-mediated genome editing leads to superior abiotic stress resiliency and distinct morphological alterations in alfalfa. 1st International Electronic Conference on Plant Science, Dec. 1 – 15, 2020

    2020 - View publication details

  18. Feyissa, B.A., Renaud, J., Nasrollahi, V., Kohalmi, S.E., Hannoufa, A. (2020). Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance, 21(1), http://dx.doi.org/10.1186/s12864-020-07118-4

    2020 - View publication details

  19. Hanly, A., Karagiannis, J., Lu, Q.S.M., Tian, L., Hannoufa, A. (2020). Characterization of the role of SPL9 in drought stress tolerance in medicago sativa, 21(17), 1-14. http://dx.doi.org/10.3390/ijms21176003

    2020 - View publication details

  20. Singer SD, Burton Hughes K, Dhariwal GK, Subedi U, Kader K, Hannoufa A, Acharya S. CRISPR/Cas9-mediated mutation of alfalfa SQUAMOSA PROMOTER-BINDING-LIKE8 alleles leads to distinct morphological alterations. Plant Biology Worldwide Summit 2020, virtual conference, July 27 – 31, 2020

    2020 - View publication details

Installation de recherche

1391, rue Sandford
London, ON N5V 4T3
Canada

Affiliations

Professeur Adjoint – University of Western Ontario

Membre – University of Saskatchewan, College of Graduate and Postdoctoral Studies

Vice Président – Canadian Association for Plant Biotechnology

Membre – Board of Directors, Plant Canada

Membre – Canadian Society of Plant Biologists

Language

Anglais