Trichostatin A increases embryo and green plant regeneration in wheat

Citation

Jiang, F., Ryabova, D., Diedhiou, J., Hucl, P., Randhawa, H., Marillia, E.F., Foroud, N.A., Eudes, F., Kathiria, P. (2017). Trichostatin A increases embryo and green plant regeneration in wheat, 36(11), 1701-1706. http://dx.doi.org/10.1007/s00299-017-2183-3

Plain language summary

Wheat is the second most cultivated cereal worldwide and represents one of the top five agri-food exports in Canada. The genetics of wheat is highly complex, and the generation of doubled haploid (DH) plants can greatly facilitate advanced research and breeding in this crop. DHs can be developed by isolating the male germ cells (microspores) from anthers and culturing them under conditions that lead to embryogenesis and subsequent haploid plant development. The haploid plants can then be doubled spontaneously or by chemical treatments. The challenge in generating wheat DHs from microspores is that different wheat varieties behave differently—not all wheat respond to the culturing conditions equally. In this work, the effect of trichostatin A (TSA), a chemical that has previously been shown to induce cell differentiation, was assessed in its ability to induce embryogenesis in wheat microspores. TSA was found to increase embryogenesis and subsequent haploid plant production in different wheat varieties, including those with an otherwise poor response to microspore culturing. By increasing the efficiency embryogenesis in wheat microspore cultures, this DH tool is made more accessible to wheat breeding and research programs.

Abstract

Key message: Chemical agents such as trichostatin A (TSA) can assist in optimization of doubled haploidy for rapid improvements in wheat germplasm and addressing recalcitrance issues in cell culture responses. Abstract: In wheat, plant regeneration through microspore culture is an integral part of doubled haploid (DH) production. However, low response to tissue culture and genotype specificity are two major constraints in the broad deployment of this breeding tool. Recently, the structure of chromatin was shown to be linked with cell transitions during tissue culture. Specifically, repression of genes that are required for cell morphogenesis, through acetylation of histones, may play an important role in this process. Reduction of histone acetylation by chemical inhibition may increase tissue culture efficiency. Here, the role of trichostatin A (TSA) in inducing microspore-derived embryos was investigated in wheat. The optimal dose of TSA was determined for wheat cultivars and subsequently validated in F hybrids. A significant increase in the efficiency of DH production was observed in both cultivated varieties and F hybrids. Thus, the inclusion of TSA in DH protocols for wheat breeding programs is advocated. 1 1

Publication date

2017-11-01