Short communication: Correlation between L-lactate concentrations in beef cattle, obtained using a hand-held lactate analyser and a lactate assay colorimetric kit
Daniela M Meléndez, Sonia Marti, Luigi Faucitano, Derek B Haley, Timothy D Schwinghamer ...
Journal of Animal Science, Volume 98, Issue Supplement_4, November 2020, Pages 296–297, https://doi.org/10.1093/jas/skaa278.535
Plain language summary
Lactate is a product of the break down of sugar in the body that results in the release of energy and the production of acid. It is used in animal research as an indicator of muscle fatigue and therefore, is a valuable indicator of cattle response to long distance transportation. Lactate can be measured using a commercially available analytical assay (considered to be the gold standard), or hand-held devices developed to be more time and cost effective than traditional analytical method. The aim of this study was to determine if the hand-held device can be used in place of the lab method by evaluating how closely matched the lactate measurements between the two methods are. Blood samples were collected from 96 steers prior to loading and after 36 hours of transportation, and prior to reloading and after an additional 4 hours of road transportation, and on days 1, 2, 3, 5, 14, and 28 after transport. At the time of blood collection, hand-held analyzer strips were dipped in blood, while additional blood was collected into tubes for analysis in the lab. Correlation between the two methods was poor leading to the conclusion that the hand held analyzer was not a suitable alternative to the gold standard lab assay.
Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from r = - 0.03 (p = 0.75; LO1) to r = 0.75 (p = < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant (r = 0.33, p < 0.001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.