Serum metabolite biomarkers for predicting residual feed intake (RFI) of young angus bulls

Citation

Foroutan, A., Fitzsimmons, C., Mandal, R., Berjanskii, M.V., Wishart, D.S. (2020). Serum metabolite biomarkers for predicting residual feed intake (RFI) of young angus bulls. Metabolites, [online] 10(12), 1-20. http://dx.doi.org/10.3390/metabo10120491

Plain language summary

A feed efficiency measure known as residual feed intake (RFI) is a commonly used in the livestock industry to identify animals that either efficiently or inefficiently convert the food they eat into meat or body mass. Selection for low residual feed intake (LRFI), or feed efficient animals, is gaining popularity among beef producers due to the fact that LRFI cattle eat less and produce less methane per unit weight gain. RFI is a difficult and time-consuming measure to perform, and therefore a simple blood test that could distinguish high-RFI (HRFI) from LRFI animals (early on) would potentially benefit beef farmers in terms of optimizing production or selecting which animals to cull or breed. Therefore, in order to identify small molecules (metabolites) in the blood that could be used as biological markers for RFI, we used three different methods to perform large-scale studies of metabolites in the blood of HRFI and LRFI young Angus bulls. We successfully identified several biomarkers for RFI that could potentially be translated to an RFI blood test. These biomarkers have high sensitivity and specificity for distinguishing between HRFI from LRFI animals. These results suggest that measuring metabolites in the blood could be used to inexpensively predict RFI in beef cattle. Further validation using larger and more diverse groups of cattle is required to confirm these findings.

Abstract

Residual feed intake (RFI) is a feed efficiency measure commonly used in the livestock industry to identify animals that efficiently/inefficiently convert feed into meat or body mass. Selection for low-residual feed intake (LRFI), or feed efficient animals, is gaining popularity among beef producers due to the fact that LRFI cattle eat less and produce less methane per unit weight gain. RFI is a difficult and time-consuming measure to perform, and therefore a simple blood test that could distinguish high-RFI (HRFI) from LRFI animals (early on) would potentially benefit beef farmers in terms of optimizing production or selecting which animals to cull or breed. Using three different metabolomics platforms (nuclear magnetic resonance (NMR) spectrometry, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and inductively coupled plasma mass spectrometry (ICP-MS)) we successfully identified serum biomarkers for RFI that could potentially be translated to an RFI blood test. One set of predictive RFI biomarkers included formate and leucine (best for NMR), and another set included C4 (butyrylcarnitine) and LysoPC(28:0) (best for LC-MS/MS). These serum biomarkers have high sensitivity and specificity (AUROC > 0.85), for distinguishing HRFI from LRFI animals. These results suggest that serum metabolites could be used to inexpensively predict and categorize bovine RFI values. Further validation using a larger, more diverse cohort of cattle is required to confirm these findings.

Publication date

2020-12-01

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