RNAi triggered systemic acquired resistance to combat emerging plant pathogens

Continuous emergence of plant pathogens has severe consequences on crop plants and plant ecosystems. Thus, novel approaches to control plant diseases that are environmentally friendly and cost-effective are needed. RNA interference (RNAi) is a natural gene regulation and antiviral response mechanism in eukaryotes. Transgenic and non-transgenic plant-based RNAi approaches have shown great potential to target specific plant pathogens. In concert with a RNAi approach, compounds that are capable of promoting plant defense mechanisms have previously proven to trigger systemic acquired resistance in plants and can also be considered. The objective of this project is to develop and assess approaches that can be used in the short term to mitigate the effects of Tomato brown rugose fruit virus (ToBRFV), which is a serious emerging threat to greenhouse tomato production in Canada. Disease regulator products such as HEADS UP (Chlorothalonil), ACTIGUARD 50WG (Acibenzolar-S-methyl) and EZ-GRO (10% Salicylic) will be tested alone and in combination with pre-market dsRNA formulations to effectively manage ToBRFV disease without compromising overall crop production and impacting its economic value.