RNA-seq analysis of plant maturity in crested wheatgrass (Agropyron cristatum L.)

Citation

Zeng, F., Biligetu, B., Coulman, B., Schellenberg, M.P., Fu, Y.B. (2017). RNA-seq analysis of plant maturity in crested wheatgrass (Agropyron cristatum L.). Genes, [online] 8(11), http://dx.doi.org/10.3390/genes8110291

Plain language summary

Crested wheatgrass breeding programs aim to develop later maturing cultivars for extending early spring grazing in Western Canada. Plant maturity is a complex genetic trait, and little is known about genes associated with late maturity in this species. An attempt was made using RNA-Seq to profile the transcriptome of crested wheatgrass maturity and to analyze differentially expressed genes (DEGs) between early and late maturing lines. This study detected 13,133 DEGs. Specifically, we found 22, 17, and eight flowering related DEGs for the three stages of plant maturity, respectively. Twelve DEGs, including nine flowering related DEGs at the stem elongation stage were further confirmed empirically. The analysis of homologous genes of the photoperiod pathway revealed their lower expression in the late maturing line at the stem elongation stage, suggesting that their differential expression contributed to late maturity in crested wheatgrass. These findings enhance our understanding of genetic basis of plant maturity and provide us basic knowledge that is helpful for its breeding program.

Abstract

Crested wheatgrass (Agropyron cristatum L.) breeding programs aim to develop later maturing cultivars for extending early spring grazing in Western Canada. Plant maturity is a complex genetic trait, and little is known about genes associated with late maturity in this species. An attempt was made using RNA-Seq to profile the transcriptome of crested wheatgrass maturity and to analyze differentially expressed genes (DEGs) between early and late maturing lines. Three cDNA libraries for each line were generated by sampling leaves at the stem elongation stage, spikes at the boot and anthesis stages. A total of 75,218,230 and 74,015,092 clean sequence reads were obtained for early and late maturing lines, respectively. De novo assembly of all sequence reads generated 401,587 transcripts with a mean length of 546 bp and N50 length of 691 bp. Out of 13,133 DEGs detected, 22, 17, and eight flowering related DEGs were identified for the three stages, respectively. Twelve DEGs, including nine flowering related DEGs at the stem elongation stage were further confirmed by qRT-PCR. The analysis of homologous genes of the photoperiod pathway revealed their lower expression in the late maturing line at the stem elongation stage, suggesting that their differential expression contributed to late maturity in crested wheatgrass.

Publication date

2017-11-01

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