Re-classification of clavibacter michiganensis subspecies on the basis of whole-genome and multi-locus sequence analyses

Citation

Li, X., Tambong, J., Yuan, K.X., Chen, W., Xu, H., André Lévesque, C., De Boer, S.H. (2018). Re-classification of clavibacter michiganensis subspecies on the basis of whole-genome and multi-locus sequence analyses. International Journal of Systematic and Evolutionary Microbiology (IJSEM), [online] 68(1), 234-240. http://dx.doi.org/10.1099/ijsem.0.002492

Plain language summary

Although bacteria of the genus Clavibacter were originally proposed to be in one species with several subspecies, recent whole-genome data and the use of multiple genes show that they are distinct species. This article reports scientific evidence to confirm that the six bacterial subspecies are authentic and unique species.

Abstract

Although the genus Clavibacter was originally proposed to accommodate all phytopathogenic coryneform bacteria containing B2γ diaminobutyrate in the peptidoglycan, reclassification of all but one species into other genera has resulted in the current monospecific status of the genus. The single species in the genus, Clavibacter michiganensis, has multiple subspecies, which are all highly host-specific plant pathogens. Whole genome analysis based on average nucleotide identity and digital DNA– DNA hybridization as well as multi-locus sequence analysis (MLSA) of seven housekeeping genes support raising each of the C. michiganensis subspecies to species status. On the basis of whole genome and MLSA data, we propose the establishment of two new species and three new combinations: Clavibacter capsici sp. nov., comb. nov. and Clavibacter tessellarius sp. nov., comb. nov., and Clavibacter insidiosus comb. nov., Clavibacter nebraskensis comb. nov. and Clavibacter sepedonicus comb. nov.

Publication date

2018-01-01

Author profiles