Protoplasts in the analysis of early plant-pathogen interactions: current applications and perspectives
Citation
Xing T., Li, X.-Q., Laroche, A., Tian, L., Tubei, K., and Wang, X. (2017) Protoplasts in the analysis of early plant-pathogen interactions: current applications and perspectives. European Journal of Plant Pathology DOI 10.1007/s10658-017-1230-9.
Plain language summary
We have reviewed the potential of transient gene expression in protoplasts (plant cells without the rigid cell wall surrounding them) to study the role of different genes in the plant responses against different pathogens. This simplified system is more efficient to initially assess the the role and function of candidate genes involve in the plant resistance response against pathogens.
Abstract
© 2017, Koninklijke Nederlandse Planteziektenkundige Vereniging. Transient gene expression in protoplasts has been applied to the analysis of gene expression, promoter activity, subcellular localization of proteins, and genome editing. Its advantages have been realized in recent years in various physiological, biochemical, genetic, and molecular biological studies. The omics and systems biology studies of plant development and their interactions with the environment have revitalized this approach. Early plant-pathogen interaction represents one of the most unique signalling research areas. Here, we try to discuss some developments and current applications of the protoplast system. It is for sure that the system will significantly bridge the gap between high throughput approaches and functional analysis in the study of plant-pathogen interactions in the omics era.