Propagation Strategies and Genetic Fidelity in Strawberries
Debnath, S.C. (2013). Propagation Strategies and Genetic Fidelity in Strawberries, 13(1-2), 3-18. http://dx.doi.org/10.1080/15538362.2012.696520
While strawberries have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. The in vitro morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, which is again genotype specific. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon a better understanding of physiological and biochemical responses of plants to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of strawberry plants in liquid culture systems. Clonal fidelity can be a serious problem and molecular strategies have been developed in order to reduce the variation to manageable levels. The article describes the progress in-depth of various aspects of strawberry propagation in vitro on semi-solid gelled media and in liquid media using bioreactors, for their improvement and for commercial production. The article also focuses on the employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability, and trueness-to-type among donor plants and tissue culture regenerant. © 2013 Copyright 2013 Crown copyright.