Potato Greening through ‘omics approaches

Citation

Potato Greening through ‘omics approaches

Abstract

Potato ‘greening’ occurs when tubers are exposed to light, and results from a de novo synthesis of chlorophyll and a simultaneous formation of steroidal glycoalkaloids, which are toxic to humans and animals. Potato is one of the four crops that supply 50% of the world’s food energy needs and the largest vegetable crop in Canada. however, greening causes substantial loss of products. Currently, there are no potato cultivars that are resistant to light–induced greening available on the market. The goal of this study is to understand the genetic components and molecular mechanisms of light-induced greening, and to use this knowledge for developing gene-editing tools aimed to generate cultivars resistant to greening. From a core germplasm collection of mutant diploid potato clones, those found to be tolerant to light-induced greening were selected. These non-greening clones, along with a greening control, underwent transcriptomic sequencing after light exposure and their whole genome sequenced. Here, we will show our findings on the transcriptomic expression and genomic variations that differentiate the non-greening from greening clones, and how this information contributed to the development of gene-editing tools targeting commercially significant potato varieties. Deploying the non-greening trait into popular potato cultivars would be of high interest to the industry and stakeholders both for tuber appearance, quality, safety, marketability, and food waste reduction.