In planta proximity dependent biotin identification (BioID)
Citation
Khan, M., Youn, J.Y., Gingras, A.C., Subramaniam, R., Desveaux, D. (2018). In planta proximity dependent biotin identification (BioID). Scientific Reports, [online] 8(1), http://dx.doi.org/10.1038/s41598-018-27500-3
Plain language summary
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of
proteome architecture, including insoluble or membrane-associated proteins. The technique has
been well established in mammalian cells but has yet to be applied to whole plant systems.
Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis
thaliana, thereby expanding the versatility of this important technique and providing a powerful
proteomics tool for plant biologists.
Abstract
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant Arabidopsis thaliana, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists.