Mutational Genetics In Diploid Potato In The CRISPR Era

Mutational Genetics In Diploid Potato In The CRISPR Era
Fofana, B.*1, A. Somalraju1, D. Main1, M. Zaidi1, B. Bizimungu2
1Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, 440 University Avenue, Charlottetown, Prince Edward Island, C1A 4N6, Canada; 2Fredeicton Research and Development Centre, Agriculture and Agri-Food Canada, 95 Innovation Road, PO Box 20280, Fredericton, NB E3B 4Z7
Cultivated potato (Solanum tuberosum L.) is the fourth most consumed food crop after rice wheat and maize. Cultivated potato is a clonally propagated, autotetraploid crop species with a narrow genetic diversity. Its highly heterozygous, complex genome, and tetrasomic inheritance make its genetic studies and improvement more difficult than grain crops. Recently, diploid potato breeding has regained an interest in the potato genetics community. Genetically, diploid potatoes are easy to work with, can be used as gene donors in the breeding process of cultivated potatoes, and can also be grown on their own as varieties. However, diploid breeding continuum faces many challenges including anti-nutritional factors and self-incompatibilities. Mutations are known as the key drivers for evolution and diversification in plants. In breeding and varietal selection, sources for variation are always sought as starting materials, and in the absence of desired natural variations in breeding populations, targeted or random mutagenesis is applied to induce variations. Recently, a mutagenized pre-breeding diploid potato population was developed at AAFC Charlottetown. Dr. Fofana will give an update on this genetic resource, its characterization, and its potentials in the CRISPR era.