High viability of dormant Malus buds after 10 years of storage in liquid nitrogen vapour.

Three hundred and sixty two Malus accessions from the Canadian Clonal Genebank of Plant Gene Resources of Canada were cryopreserved as dormant buds at the USDA-ARS National Center for Genetic Resources Preservation in 1996. According to grafting data collected on 165 of these accessions in 1999, 80% of the accessions had at least 40% viability. A subsample of these accessions was processed for cryopreservation by either adjusting the moisture content of the budwood sections containing dormant buds to 32 or 37% moisture (fresh weight basis) or by not drying the budwood sections (46% moisture fresh weight basis) prior to cooling. Budwood sections were then slow-cooled at VC h⁻¹ to -30 degrees C, held for 24 h at -30 degrees C and then rapidly transferred to the vapour phase of liquid nitrogen. Cryopreserved buds from 13 accessions that were dried using the various techniques were warmed and grafted in both 1999 and 2006 to determine viability. Overall, bud viability was hi gh at both storage times. At the 10 year timepoint, some accessions had higher bud growth when they were desiccated prior to slow-cooling when compared to those that were not.