Genome-wide identification of cytochrome P450 monooxygenases provide insight into their role in partial resistance against Phytophthora sojae in soybean (Glycine max).
Khatri, P.* and Dhaubhadel, S. (2021) Genome-wide identification of cytochrome P450 monooxygenases provide insight into their role in partial resistance against Phytophthora sojae in soybean (Glycine max). Phytochemical Society of North America 2021. Kelowna, BC, CA, in a virtual format, July 25-30.
Plain language summary
Cytochrome P450 consists of a group of enzymes that are involved in the synthesis of many compounds produced by plants during stress. By the bioinformatic analysis, we found that soybean contains 346 of such enzymes where 103 may have a function in resistance against stem and root rot disease caused by Phytophthora sojae.
Cytochrome P450 monooxygenases have proven their potential in synthesis of many secondary metabolites involving alkaloids, terpenoids, phytoalexins and saponins. Plants utilize these metabolites to combat with biotic as well as antibiotic stress. In our study we have found 359 GmP450 corresponding to 342 genes participating in many metabolic pathways in soybean specifically explored their role in partial resistance against Phytophthora sojae. The genome wide comparison of A-type and non-A-type GmP450s was also performed in respect to their motifs, exon-intron structure, tissue specific expression and their distribution over chromosomes. The higher number of exon-intron distribution clearly pointed that most non-A type GmP450s originated recently. The analysis of motifs showed a conserved EXXR, PXRX and CXG motifs among all GmP450s. There is more variation found in non-A type as compared to A-type GmP450s. For the first time we analysed variable region within different motifs of P450s and discovered that in A-type CYP families, the 51 amino acids variable region were found between the EXXR and oxygen binding domains and PXRX and EXXR domains, respectively with some exceptions while non–A type showed wide variation. The analysis of transcriptomic datasets resulted out in 21 GmP450s inducing upon P. sojae infection in soybean expressing higher in root tissue. The analysis of different partial resistance QTLs against P. sojae resulted out in 139 GmP450s falling under QTL regions furthermore out of those 103 GmP450s found induced upon P. sojae infection. The overall analysis of GmP450s in soybean showed that members of CYP71, CYP82 and CYP93 actively participate in partial resistance mechanism in soybean to provide inherent immunity to plant.