Genome sequences of isolates from high-touch surfaces in washrooms at a post-secondary institution

Citation

Holman, D.B., James-Gzyl, K.E., Rieberger, R.L., Keim, K.S., Carson, M.J., Norris, S.G., Taheri, A.E. (2023). Genome sequences of isolates from high-touch surfaces in washrooms at a post-secondary institution. Microbiology Resource Announcements, [online] 12(12), http://dx.doi.org/10.1128/MRA.00910-23

Plain language summary

High-touch objects (paper towel dispenser handle, soap dispenser handle, and toilet seat) in public washrooms on the Red Deer Polytechnic main campus in Red Deer, Alberta, Canada, were swabbed and streaked onto CHROMagar MRSA (methicillin-resistant Staphylococcus aureus) with the objective of detecting MRSA or other methicillin-resistant Staphylococcus spp. Twelve isolates were recovered, sequenced, and identified as Brevibacterium casei (n = 1), Heyndrickxia oleronia (n = 1), Kocuria palustris (n =1), Microbacterium spp. (n = 5), Staphylococcus cohnii (n = 3), and Staphylococcus epidermidis.
Although all isolates grew on CHROMagar MRSA, none were identified as S. aureus after genome sequencing. However, all Staphylococcus cohnii (n = 3) and Staphylococcus epidermidis (n = 1) genomes carried the mecA gene for methicillin resistance which may explain their selection on CHROMagar MRSA. The Microbacterium spp. isolates appeared to belong to a novel species.

Abstract

We report here the draft genome sequences of Brevibacterium casei (n = 1), Heyndrickxia oleronia (n = 1), Kocuria palustris (n =1), Microbacterium spp. (n = 5), Staphylococcus cohnii (n = 3), and Staphylococcus epidermidis isolated from high-touch surfaces in washrooms at a post-secondary institution.

Publication date

2023-12-01

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