Generation and development of diploid potato lines producing tubers low in glycoalkaloid and asparagine after ethyl methane sulfonate mutagenesis

Citation

Generation and development of diploid potato lines producing tubers low in glycoalkaloid and asparagine after ethyl methane sulfonate mutagenesis

Abstract

Potato constitutes a major component of the human diet. However, depending on varieties and handling, potato may contain high levels of anti-nutritional factors including acrylamide and steroidal glycoalkaloids (SGA). Whereas backcrossing breeding strategies contributed to releasing varieties with low SGA and acrylamide-forming potential, substantial resources are still required to minimizing these anti-nutritional factors. We developed and characterized an EMS mutagenized potato population using a reverse and forward genetics platform. A subset of 1,750 EMS-treated and 140 non-treated lines underwent reverse genetics through amplicon sequencing of AS1, AS2, SGT1, and SGT2 for the detection of EMS-induced mutations. A mutation frequency ranging from 1/43 kb to 1/436 kb was determined in the 4 genes among 208 EMS lines and 124 missense and 24 nonsense mutations were detected in exonic regions. Linking phenotype to genotype, large variations in SGA and asparagine content as well as for late blight resistance were observed. The data will be presented and discussed in a context of green breeding strategy.

Publication date

2017-06-26

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