Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine

Citation

Dalto, B.D., Tsoi, S., Audet, I., Dyck, M.K., Foxcroft, G.R., Matte, J.J. (2015). Gene expression of porcine blastocysts from gilts fed organic or inorganic selenium and pyridoxine, 149(1), 31-42. http://dx.doi.org/10.1530/REP-14-0408

Abstract

In this study, we determined how maternal dietary supplementation with pyridoxine combined with different sources of selenium (Se) affected global gene expression of porcine expanded blastocysts (PEB) during pregnancy. Eighteen gilts were randomly assigned to one of the three experimental diets (nZ6 per treatment): i) basal diet without supplemental Se or pyridoxine (CONT); ii) CONT+0.3 mg/kg of Na-selenite and 10 mg/kg of HCl-pyridoxine (MSeB 10); and iii) CONT+0.3 mg/kg of Se-enriched yeast and 10 mg/kg of HCl-pyridoxine (OSeB 10). All gilts were inseminated at their fifth post-pubertal estrus and killed 5 days later for embryo harvesting. A porcine embryo-specific microarray was used to detect differentially gene expression between MSeB 10 vs CONT, OSeB 10 vs CONT, and OSeB 10 vs MSeB 10. CONT gilts had lower whole blood Se and erythrocyte pyridoxal-5-P concentrations than supplemented gilts (P<0.05). No treatment effect was observed on blood plasma Se-glutathione peroxidase activity (PZ0.57). There were 10, 247, and 96 differentially expressed genes for MSeB 10 vs CONT, OSeB 10 vs CONT, and OSeB 10 vs MSeB 10 respectively. No specific biological process was associated with MSeB 10 vs CONT. However, for OSeB 10 vs CONT, upregulated genes were related with global protein synthesis but not to selenoproteins. The stimulation of some genes related with monooxygenase and thioredoxin families was confirmed by quantitative real-time RT-PCR. In conclusion, OSeB 10 affects PEB metabolism more markedly than MSeB 10. Neither Se sources with pyridoxine influenced the Se-glutathione peroxidase metabolic pathway in the PEB, but OSeB 10 selectively stimulated genes involved with antioxidant defense. 6 6 6 6 6 6 6 6 6 6 6 6 6 6 6

Publication date

2015-01-01