Exploring the genetic diversity and mycotoxigenic potential of Canadian Fusarium poae isolates.

Citation

Harris, L.J., Johnston, A., Hermans, A., Sproule, A., Xue, A., Overy, D. Exploring the genetic diversity and mycotoxigenic potential of Canadian Fusarium poae isolates. 9th Can. Workshop on Fusarium Head Blight & 4th Can. Wheat Symp., https://www.cwfhb-cws.com/hotel-1/ Winnipeg, MB, Nov 19-22, 2018. P. 51. Poster.

Abstract

FHB-damaged grain is often co-contaminated with a number of Fusarium species, including Fusarium graminearum (main causal agent of FHB), Fusarium avenaceum, and Fusarium poae. These species are each capable of producing a diverse set of mycotoxins and other secondary metabolites. Recent Ontario surveys suggest that F. poae is the main fungus isolated from oats while F. poae and F. graminearum share the title of most commonly isolated from barley. European and Asian isolates of F. poae have been reported to produce type A and type B trichothecenes as well as beauvericin, cyclonerodiol, and enniatins, but little is known about the mycotoxigenic potential of Canadian isolates. We are interested in the genetic diversity and mycotoxin profile of the Canadian F. poae population. A set of 207 F. poae monosporic strains were obtained from oat, barley and wheat samples collected in several Canadian provinces (primarily ON but also QC and SK, 2006-2017 field seasons). Sequencing the tef1╬▒ gene confirmed the species identification and sequencing two trichothecene biosynthetic genes (TRI1 and TRI8) allowed assignment of the isolates into 13 groups. Metabolomic profiling on YES agar demonstrated the chemotype diversity of an initial set of 160 strains and the production of certain trichothecenes and emerging mycotoxins was confirmed. Emerging mycotoxins diacetoxyscirpenol and beauvericin were consistently produced by all strains. A representative subset of 46 strains was chosen for intensified metabolomics profiling and genome sequencing (NextSeq).

Publication date

2018-11-19