Evaluation of the effects of five sumai 3 QTL on fusarium head blight resistance in Canadian spring wheat

Citation

Berraies, S., Knox, R.E., DePauw, R.M., Clarke, F.R., Singh, A., Cuthbert, R.D., Martin, R.A., Xue, A., Gilbert, J.A., Brule Babel, A.L., and Campbell H. 2016. 1b28. Evaluation of the effects of five sumai 3 QTL on fusarium head blight resistance in Canadian spring wheat. 5th International Symposium on Fusarium Head Blight, Florianopolis. April 6-9.

Abstract

The cultivar Sumai 3 is a commonly used source of resistance in wheat (Triticum aestivum L.) breeding programs. The aim of this study was to evaluate the effect of Sumai 3 QTL to validate the transmission of loci that contribute to Type I and Type II FHB resistance, and Fusarium damaged kernel (FDK) and deoxynivalenol (DON) reduction in breeding populations through Sumai 3 derived parents. The three doubled haploid (DH) populations, Infinity/ND3085, Infinity/ND744 and Alsen/Helios utilizing Sumai 3 derived parents Alsen, ND3085 and ND744 were evaluated. In 2007 and 2008, 80 DH lines of each population and the parental genotypes were grown in FHB screening nurseries near Carman MB, Ottawa, ON and Charlottetown, PEI. The percentage of incidence (Type I), severity (Type II), FDK and DON accumulation were measured and FHB index calculated. DNA markers at five FHB resistance QTL detected in Sumai 3 on chromosomes 2DL, 3BS, 4B, 5AS, and 6BS were evaluated on the populations. For each trait, a t-test was applied to means of observations pooled by parental type of each marker to determine which loci contributed to resistance. The alleles at the 3BS and 5AS genomic regions most frequently contributed to Type I and Type II FHB resistance as well as to a reduced FDK and DON in all three populations. Interaction between loci across populations and environments was observed. Over the years and across the environments some loci showed a consistent effect and strong association with resistance, while the effect of some other loci was infrequent and irregular. The results of this study showed that resistance loci from Sumai 3 at the 3BS and 5AS QTL were transmitted to the progeny from the resistant parents to effectively reduced FHB damage.