Efficacy of multi-stage sous-vide cooking on tenderness of low value beef muscles

Citation

Uttaro, B., Zawadski, S., McLeod, B. (2019). Efficacy of multi-stage sous-vide cooking on tenderness of low value beef muscles. Meat Science, [online] 149 40-46. http://dx.doi.org/10.1016/j.meatsci.2018.11.008

Plain language summary

Two sous-vide cooking methods were tested for how effective they were in increasing tenderness of Canada AA beef chuck tenders (supraspinatus) and beef knuckles (rectus femoris) aged for 10 d. One method cooked vacuum-packaged steaks at 59C for 4 h (single-stage sous-vide: S-SV), and the other cooked them at 39C for 1 h, then at 49C for another 1h, and finally at 59C for 4 h (multi-stage sous-vide: M-SV). The control treatment (Ctl) was cooking bagged steaks in 70C water until they reached 59C (~22 min). Steaks were cooled rapidly following cooking, then refrigerated for up to 2 wks. Meat was then reheated on a grill to an internal temperature of 55C, and while still warm, underwent tenderness testing. The results showed that S-SV meat was 17-21% more tender than Ctl, and that M-SV became a further 5-6% more tender. The changes seen appeared to be consistent with the action of residual native enzymes in the meat, which, when selectively activated by specific temperatures, worked to break down muscle fibers and the connective tissue holding them together. The practical implication is that a cut which is lower value because of toughness could possibly be substituted for a higher value cut if the lower value cut is cooked with the M-SV method.

Abstract

The efficacy of thermal activation of residual proteolytic enzymes on shear force and deformation of 72 beef supraspinatus (SS) and rectus femoris (RF) muscles was tested using multi-stage sous-vide cooking (M-SV; 1 h at 39 °C, 1 h at 49 °C, 4 h at 59 °C), single-stage sous-vide cooking (S-SV; 4 h at 59 °C), and waterbath cooking (22 min at 70 °C, to 59 °C). Two storage conditions (1 week at 2 °C; 2 weeks at −1.5 °C) followed, then meat was reheated to and tested at 55 °C. Shear force decreased by 17–21% with S-SV (P < 0.001) and appeared to affect both myofibrillar and collagen components, likely through heat activation of cathepsin B & L and 20S proteasome. A further 5–6% increase was realized with M-SV (P: SS = 0.006, RF = 0.12) affecting primarily the myofibrillar component, likely from calpain-2 activation. The degree of deformation increased with sous-vide cooking in general (P < 0.001). No meaningful effects of post-cooking storage were found (P > 0.05).

Publication date

2019-03-01

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