Effects of redox modifications on proteins encoded by the Low expression of OSmotically responsive genes 2 locus in Arabidopsis thaliana
Ouellet J, Dumont S, Bykova NV, Rojas LM, Dorion S, Bélanger M, Kornblatt, MJ, Rivoal J (2020) Effects of redox modifications on proteins encoded by the Low expression of OSmotically responsive genes 2 locus in Arabidopsis thaliana. Concours d'affiches scientifiques étudiantes 2020 du Centre SÈVE, Virtual Meeting, Québec, Canada, November 25-26, 2020. Poster, student
To tolerate diverse environmental conditions, plants have developed mechanisms ensuring an efficient management of reactive oxygen and nitrogen species. These small molecules play an important signaling function through redox-dependent post-translational modifications. Modifications, such as S-glutathionylation, have a protective role against irreversible oxidation of proteinic thiols and implications in the regulation of protein activity and function. This research investigates redox sensitivity of proteins encoded by the Low expression of OSmotically responsive genes 2 gene of Arabidopsis thaliana. This gene encodes an mRNA from which two proteins can be differentially translated. The first is a cytosolic enolase which is responsible for most of the cellular phosphoenolpyruvate production. The second is a transcription factor implicated in cold response regulation. Recombinant versions of both proteins were produced and subjected to in vitro assays in order to evaluate their sensibility to redox conditions. In spite of their divergent functions, these proteins exhibit some similarities in their sensitivity to oxidative conditions. In particular, our results support the existence of a covalent modification with glutathione for both proteins. In the end, this research could lead to a better understanding of redox regulation events in plant primary metabolism and in response to environmental stress.