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Citation
2021
Plain language summary
Isolated microspore culture (IMC) for the production of doubled haploid (DH) plants is a method used by plant breeders to rapidly obtain homozygous breeding lines. Breeders cut many years off the time it takes to create commercial lines by recovering plants from chromosome doubling of haploids. Although there are several DH production methods, IMC has great potential and is highly desirable because large numbers of cells can be isolated and cultured with relative ease. Even with this advantage, however, several factors inhibit universal adoption of microspore culture, especially in wheat. Problematic areas are the rapid death of microspores in culture, the failure of microspore to undergo embryogenesis and the high percentage of albinos that can be seen in resulting DH plants of certain genotypes. This chapter highlights these challenges and reviews research being done towards finding solutions for IMC improvement with a focus on wheat.
Abstract
Isolated microspore culture (IMC) for the production of doubled haploid (DH) plants is a method used by plant breeders to rapidly obtain homozygous breeding lines. Breeders cut many years off the time it takes to create commercial lines by recovering plants from chromosome doubling of haploids. Although there are several DH production methods, IMC has great potential and is highly desirable because large numbers of cells can be isolated and cultured with relative ease. Even with this advantage, however, several factors inhibit universal adoption of microspore culture, especially in wheat. Problematic areas are the rapid death of microspores in culture, the failure of microspore to undergo embryogenesis and the high percentage of albinos that can be seen in resulting DH plants of certain genotypes. This chapter highlights these challenges and reviews research being done towards finding solutions for IMC improvement with a focus on wheat.