Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance

Citation

Bipfubusa, M., Rocher, S., Bertrand, A., Castonguay, Y., Renaut, J. (2016). Dataset of protein changes induced by cold acclimation in red clover (Trifolium pratense L.) populations recurrently selected for improved freezing tolerance, 8 570-574. http://dx.doi.org/10.1016/j.dib.2016.06.003

Plain language summary

Improving Red Clover’s freezing tolerance (Trifolium pratense L.) would increase its persistence in harsh winter conditions. In this study, we assessed freezing tolerance and compared the set of proteins expressed in non-acclimated and cold-acclimated plants. This comparison also included initial red clover cultivars Endure (E-TF0) and Christie (C-TF-0) and populations selected for improved freezing tolerance after either 3 (TF3) or 4 (TF4) cycles of selection. Plants were hardened to natural variation in air and soil temperatures throughout fall and winter into an unheated greenhouse. Freezing tolerance was assessed before cold acclimation in October and in January when plants were fully cold acclimated. In October, red clover was killed at around -2°C while in January plants survived -16 °C. Plants selected for improved freezing tolerance (TF4) could survive 2 to 3°C lower than the initial cultivars. We also found that cold acclimation had a very large impact on the proteins expressed in the plants. Marked increases in vegetative storage proteins (VSP), involved in plant regrowth in the spring, and dehydrins, involved in plant cold resistance, were among the most striking changes in protein composition of cold acclimated red clovers. A subset of proteins varied in abundance in response to selection including a dehydrin that increased in abundance in TF3 and TF4 populations as compared to TF0 in cultivar Endure. Our observations suggest that variations in freezing tolerance in red clover are associated with specific proteins changes,

Abstract

© 2016 .The data provide an overview of proteomic changes in red clover (Trifolium pratense L.) in response to cold acclimation and recurrent selection for superior freezing tolerance. Proteins were extracted from crowns of two red clover cultivars grown under non-acclimated or cold-acclimated conditions, and plants obtained from the initial genetic background (TF0) and from populations obtained after three (TF3) and four cycles (TF4) of recurrent selection for superior freezing tolerance. Proteins were analyzed using a two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) coupled to mass spectroscopy (MS and MS/MS). Differentially regulated proteins were subsequently identified using MALDI TOF/TOF analysis. The data are related to a recently published research article describing proteome composition changes associated with freezing tolerance in red clover, "A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)" (Bertrand et al., 2016 [1]). They are available in the ProteomeXchange Consortium database via the PRIDE partner repository under the dataset identifier PRIDE: PXD003689.

Publication date

2016-09-01