Cloning and functional characterization of a chalcone isomerase from trigonella foenum-graecum L

Citation

Qin, J.C., Zhu, L., Gao, M.J., Wu, X., Pan, H.Y., Zhang, Y.S., Li, X. (2011). Cloning and functional characterization of a chalcone isomerase from trigonella foenum-graecum L. Planta Medica, [online] 77(7), 765-770. http://dx.doi.org/10.1055/s-0030-1250566

Abstract

Flavonoids belong to a group of plant natural products with variable phenolic structures and play important roles in protection against biotic and abiotic stress. Fenugreek (Trigonella foenum-graecum L.) seeds and stems contain flavonol glycosides and isoflavone derivatives. Up to now, the molecular features of fenugreek flavonoid biosynthesis have not been characterized. Here we present cloning of a cDNA encoding a chalcone isomerase (namely TFGCHI1) from the leaves of T. foenum-graecum which convert chalcones to flavanones in vitro. Transformation of Arabidopsis loss-of-function tt5 (chi) mutant with a TFGCHI1 cDNA complemented tt5 and produced higher levels of flavonol glycosides than wild-type Col-0. © Georg Thieme Verlag KG Stuttgart · New York.

Publication date

2011-01-01

Author profiles