Bioreactor micropropagation strategies and epigenetic variations in berry crops
Citation
Debnath, S.C. (2018). Bioreactor micropropagation strategies and epigenetic variations in berry crops. In Vitro Cell. Develop. Biol. - Plant 54 (Suppl 1):S1; https://doi.org/10.1007/s11627-018-9927-9; 2018 Int. Assoc. Plant Biotechnol. Congr. Abst. Issue, P-23; S39; Abstract; http://iapb2018.com/wp-content/uploads/2018/08/IAPB2018-Book-of-Abstracts.pdf.
Plain language summary
Conference presentation containing research updates on micropropagation of berry crops and epigenetic variation in micropropagated berry crops.
Abstract
Micropropagation is a multibillion dollar industry throughout the world. Automated bioreactors using liquid media permit multiplication of berry plants as a possible way of reducing propagation cost. Success in bioreactor micropropagation depends upon better understanding of explant response for physiological and biochemical factors to the signals of liquid culture
microenvironment. Although both stationary (SB) and temporary immersion bioreactor (TIB) systems are successful for berry crop micropropagation, use of Growtek SB bioreactors
will reduce the micropropagation cost significantly compared to the TIB bioreactor systems. Somaclonal variation is a major concern in commercial micropropagation to produce
true-to-type micropragules. It is unpredictable in nature, can be both heritable (genetic) and non-heritable (epigenetic), and may also result in a range of genetically stable variation useful in berry crop improvement. Epigenetic modifications that include DNA methylationinduced
changes may be due to activation of transposable elements, chromosome aberration and/or gene mutation. The presentation describes the progress of various aspects of bioreactor micropropagation, and molecular markers used for clonal fidelity and epigenetic variation in berry crop micropropagation.