Stacy D. Singer, Ph.D.
Current research and/or projects
Crop species face numerous types of abiotic and biotic pressures, which often severely limit productivity and are predicted to become even more problematic in coming years due to climate change. In addition, inadequacies in forage quality can lead to substantial economic losses for producers and the emission of significant quantities of greenhouse gases. As such, there is a very diverse range of traits that could be enhanced to improve sector profitability, sustainability and competitiveness, and also reduce environmental impact. To address these challenges, my research program focuses on unraveling the genetic mechanisms driving various performance- and quality-related traits, especially in forage crops, as well as the improvement of these traits using advanced molecular breeding tools such as genome editing.
Research and/or project statements
- developing genome editing technology in forage crop species to increase the pace and precision of breeding
- improving abiotic stress tolerance to increase climate change resiliency
- enhancing photosynthetic efficiency/capacity to increase productivity
- boosting oil production in vegetative tissues to reduce methane emissions from livestock production
Education and awards
PhD, Plant Molecular Genetics, University of Regina (2005). Received the President's Distinguished Graduate Student Award.
BSc (Great Distinction), Biology (Major), Biochemistry (Minor), University of Regina (1999).
Subedi U, Burton Hughes K, Dhariwal G, Kader K, Hannoufa A, Acharya S, Singer SD. Mutation of MsSPL8 alleles via CRISPR/Cas9-mediated genome editing leads to superior abiotic stress resiliency and distinct morphological alterations in alfalfa. 1st International Electronic Conference on Plant Science, Dec. 1 – 15, 20202020 - View publication details
Subedi U, Burton Hughes K, Dhariwal G, Chen G, Acharya S, Singer SD. Transcriptional down-regulation of various genes in alfalfa enhances tolerance to abiotic stresses. 1st International Electronic Conference on Plant Science, Dec. 1 – 15, 20202020 - View publication details
Moving toward climate-resilient crops and sustainable agriculture. Research Outreach (https://researchoutreach.org/wp-content/uploads/2020/07/Stacy-Singer.pdf)2020 - View publication details
Singer SD, Burton Hughes K, Dhariwal GK, Subedi U, Kader K, Hannoufa A, Acharya S. CRISPR/Cas9-mediated mutation of alfalfa SQUAMOSA PROMOTER-BINDING-LIKE8 alleles leads to distinct morphological alterations. Plant Biology Worldwide Summit 2020, virtual conference, July 27 – 31, 20202020 - View publication details
Subedi U, Dhariwal GK, Burton Hughes K, Chen G, Acharya S, Singer SD. RNAi mediated down-regulation of various genes enhances abiotic stress tolerance in alfalfa. Plant Biology Worldwide Summit 2020, virtual conference, July 27 – 31, 20202020 - View publication details
Singer SD, Subedi U, Feyissa BA, Dhariwal GK, Burton Hughes K, Kader K, Hannoufa A, Acharya S. Comparative physiological, biochemical and transcriptomic analyses of Medicago sativa subsp. falcata and subsp. sativa in the context of drought tolerance. Plant Biology Worldwide Summit 2020, virtual conference, July 27 – 31, 20202020 - View publication details
Wijekoon, C., Singer, S.D., Weselake, R.J., Petrie, J.R., Chen, G., Singh, S., Eastmond, P.J., Acharya, S.N. (2020). Downregulation of key genes involved in carbon metabolism in Medicago truncatula results in increased lipid accumulation in vegetative tissue, 60(4), 1798-1808. http://dx.doi.org/10.1002/csc2.201242020 - View publication details
Xu Y, Singer SD, Chen G. Improving oil production in plants and microalgae by engineering performance-enhanced diacylglycerol acyltransferase 1. Inform magazine, June 2020 issue2020 - View publication details
Laroche A, Singer S, Foroud N, Laurie J, Pittman A. 2020. Modern studies in Crop Sciences / Études Contemporaines chez les Grandes Cultures. Lethbridge AgExpo 2020, Lethbridge, AB, February 26-28, 2020.2020 - View publication details
Singer, S.D., Soolanayakanahally, R.Y., Foroud, N.A., Kroebel, R. (2020). Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2, 251(1), http://dx.doi.org/10.1007/s00425-019-03301-42020 - View publication details
Wijekoon, C., Singer, S.D., Weselake, R.J., Subedi, U., Acharya, S.N. (2019). Evaluation of virus-induced gene silencing methods for forage legumes including alfalfa, sainfoin, and fenugreek, 99(6), 917-926. http://dx.doi.org/10.1139/cjps-2018-03292019 - View publication details
Singer, S.D., Hannoufa, A., Acharya, S. (2018). Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment, 41(9), 1955-1971. http://dx.doi.org/10.1111/pce.130902018 - View publication details
- Adjunct status at the University of Lethbridge, Department of Biological Sciences
- Adjunct status at the University of Alberta, Department of Agricultural, Food and Nutritional Sciences