Ron Reade

Image Ron Reade
Curator of the Canadian Plant Virus Collection

The Canadian Plant Virus Collection (CPVC) maintains viable virus isolates infecting crops of economic importance in Canada (with a particular emphasis on horticultural crops) and provides access to their genetic information.  The CPVC supports  Agriculture & Agri-Food Canada priorities, in particular those aimed at addressing threats to the value chain.  It is an invaluable resource by providing positive controls for surveys/diagnostic research, and by helping evaluate the diversity of plant viruses and the emergence of virulent strains.  . The CPVC is the largest collection of its kind in Canada and is both a repository and distributor of viral genetic resources for Canadian and international researchers and diagnostic groups. The CPVC currently has 746 accessions consisting of both freeze dried (461) and living infected hosts (285) and includes many species significant to Canadian agriculture with representatives from quarantined species, and material preserved for genetic resource purposes.Clients include researchers from AAFC, other federal and provincial departments, universities and the private sector.

Current research and/or projects

  • Curator of the Canadian Plant Virus Collection (CPVC)
  • Contributing to the “Emerging Fruit Tree Decline” project

Research and/or project statements

  • Molecular virology
  • Plant-virus interactions
  • Virus characterization and analysis

Education and awards

BSc. Microbiology, University of British Columbia, Vancouver, B.C., 1993

Co-Recipient of Agriculture and Agri-Food Canada’s Outstanding Achievement in Science Award (2017) 

Key publications

  1. Alam, S.B., Reade, R., Maghodia, A.B., Ghoshal, B., Theilmann, J., Rochon, D.A. (2021). Targeting of cucumber necrosis virus coat protein to the chloroplast stroma attenuates host defense response. Virology, [online] 554 106-119. http://dx.doi.org/10.1016/j.virol.2020.10.012

    2021 - View publication details

  2. Alam.S.B., Reade, R. and Rochon, D (2016) A highly basic “KGRKPR” sequence in the coat protein of Cucumber necrosis virus plays an important role in the assembly of virions and encapsidation of full length viral RNA. 35th Annual Meeting of the American Society for Virology, Virginia Tech, Blacksburg, Virginia

    2020 - View publication details

  3. MacDonald, J.L., Punja, Z.K., Xiang, Y., Bouthillier, M.J., Reade, R., DeYoung, R.M., Bhagwat, B., Betz, E.C., Li, Y.Q., Chen, X. (2020). First report of wasabi mottle virus causing ringspot and vein-clearing symptoms on wasabi (Wasabia japonica) in North America, http://dx.doi.org/10.1080/07060661.2020.1805642

    2020 - View publication details

  4. Alam, S.B., Reade, R., Theilmann, J., Rochon, D.A. (2017). Evidence for the role of basic amino acids in the coat protein arm region of Cucumber necrosis virus in particle assembly and selective encapsidation of viral RNA. Virology, [online] 512 83-94. http://dx.doi.org/10.1016/j.virol.2017.09.003

    2017 - View publication details

  5. Rochon, D., Ghoshal, K., Theilmann, J., and Reade, R. (2015). "Cucumber necrosis virus encapsidates host RNA during infection.", 34th Annual Meeting of American Society for Virology, London, Ontario, Canada, July 11-15, 2015. (Abstract)

    2015 - View publication details

  6. Alam, S.B., Maghodia, A., Ghoshal, B., Reade, R., Theilmann, J., and Rochon, D. (2015). "Investigating the role of Cucumber necrosis virus in attenuating host defense responses during infection.", 34th Annual Meeting of American Society for Virology, Western University, London, Ontario, Canada, July 11-15, 2015. (Abstract)

    2015 - View publication details

  7. Ghoshal, K., Theilmann, J., Reade, R., Maghodia, A., Rochon, D. (2015). Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection. Journal of Virology, [online] 89(21), 10748-10761. http://dx.doi.org/10.1128/JVI.01466-15

    2015 - View publication details

  8. Rochon, D., Singh, B., Theilmann, J., Reade, R., Ghoshal, K., Alam, S.B., and Maghodia, A. (2014). "Cucumber necrosis virus infection results in de novo generation of peroxisomes from the endoplasmic reticulum and oxidative stress.", XVIth International Congress of Virology - International Union of Microbiological Societies (IUMS 2014), July 27 - August 1, 2014, Montréal, Québec, Canada, July 27-August 1, 2014.

    2014 - View publication details

  9. Rochon, D., Singh, B., Reade, R., Theilmann, J., Ghoshal, K., Alam, S.B., Maghodia, A. (2014). The p33 auxiliary replicase protein of Cucumber necrosis virus targets peroxisomes and infection induces de novo peroxisome formation from the endoplasmic reticulum. Virology, [online] 452-453 133-142. http://dx.doi.org/10.1016/j.virol.2013.12.035

    2014 - View publication details

  10. Ghoshal, K., Theilmann, J., Reade, R., Sanfacon, H., Rochon, D. (2014). The Cucumber leaf spot virus p25 auxiliary replicase protein binds and modifies the endoplasmic reticulum via N-terminal transmembrane domains. Virology, [online] 468-470 36-46. http://dx.doi.org/10.1016/j.virol.2014.07.020

    2014 - View publication details

  11. Ghoshal, K., Reade, R., Theilmann, J., Lee, J., and Rochon, D. (2012). "Identification of the origin of assembly sequence on Cucumber necrosis virus RNA.", 31st Annual Meeting of American Society for Virology, University of Wisconsin-Madison, Madison, WI, USA, July 21-25, 2012.

    2012 - View publication details

  12. Ghoshal, K., Theilmann, J., Reade, R., and Rochon, D. (2011). "Cucumber leaf spot virus replication associated protein p25 targets the endoplasmic reticulum.", XV International Congress of Virology, Sapporo, Japan, September 11-16, 2011. (Poster)

    2011 - View publication details

  13. Hao, X., Lu, A., Sokal, N., Bhagwat, B., Leung, E., Mao, R., Reade, R., Wu, Y., Rochon, D., Xiang, Y. (2011). Cucumber necrosis virus p20 is a viral suppressor of RNA silencing. Virus Research, [online] 155(2), 423-432. http://dx.doi.org/10.1016/j.virusres.2010.11.011

    2011 - View publication details

  14. Theilmann, J., Reade, R., and Rochon, D. (2010). "Coat protein of a Cucumber leaf spot virus mutant deficient in virus assembly accumulates on the chloroplast outer envelope.", 29th Annual Meeting of American Society for Virology, Montana State University, Bozeman, MT, USA, July 17-21, 2010.

    2010 - View publication details

  15. Reade, R., Kakani, K., Rochon, D. (2010). A highly basic KGKKGK sequence in the RNA-binding domain of the Cucumber necrosis virus coat protein is associated with encapsidation of full-length CNV RNA during infection. Virology, [online] 403(2), 181-188. http://dx.doi.org/10.1016/j.virol.2010.03.045

    2010 - View publication details

  16. Sharma, R., Stanford, K.I.M., Louie, M., Munns, K., John, S.J., Zhang, Y., Gannon, V.P.J., Chui, L., Reade, R., Topp, E., and McAllister, T.A. (2009). "Escherichia coli O157:H7 Lineages in Healthy Beef and Dairy Cattle and Clinical Human Cases in Alberta, Canada.", Journal of Food Protection, 72(3), pp. 601-607.

    2009 - View publication details

  17. Kakani, K., Reade, R., Katpally, U., Smith, T., Rochon, D. (2008). Induction of particle polymorphism by Cucumber necrosis virus coat protein mutants in vivo. Journal of Virology, [online] 82(3), 1547-1557. http://dx.doi.org/10.1128/JVI.01976-07

    2008 - View publication details

  18. Rochon, D., Xiang, Y., Hui, E., Theilmann, J., Orban, S., Reade, R., and White, G.J. (2007). "Subcellular targeting and host protein interactions during Cucumber necrosis virus infection.", 9th Annual Meeting of the Canadian Crop Genomics Initiative (CCGI), Penticton, BC, Canada, September 12-13, 2007.

    2007 - View publication details

  19. Xiang, Y., Bhavana, S., Reade, R., Theilmann, J., and Rochon, D. (2007). "Cucumber necrosis virus P33 is associated with the induction of necrotic symptoms in leaves of infected Nicotiana benthamiana.", 26th Meeting of the American Society for Virology, Oregon State University, Corvallis, OR, USA, July 14-18, 2007.

    2007 - View publication details

  20. Katpally, U., Kakani, K., Reade, R., Dryden, K., Rochon, D., Smith, T.J. (2007). Structures of T = 1 and T = 3 Particles of Cucumber Necrosis Virus: Evidence of Internal Scaffolding. Journal of Molecular Biology, [online] 365(2), 502-512. http://dx.doi.org/10.1016/j.jmb.2006.09.060

    2007 - View publication details

Research facility

4200 Highway #97
Summerland, BC V0H 1Z0
Canada

Language

English