Rima Menassa Ph.D.

Image Rima Menassa
Research Scientist

Current research and/or projects

Technology development of alternatives to subtherapeutic and metaphylactic antimicrobials used in the beef industry (2016-2022)

RNAi trap plants for horticulture (2018-2021)

Mitigating AMR (AAFC-GRDI) (2018-2023)

Development of an oral intervention method to eliminate shedding of Enterohemorrhagic Escherichia coli O157:H7 by pigs. (Collaboration with PlantForm Corp.) (2020-2022)

Plant-based oral vaccine for PEDv disease. (AAFC AgriScience project) (2019-2022)

Research and/or project statements

Plants as bioreactors for the production of veterinary vaccines and antibodies as alternatives to antibiotics

Transforming the chloroplast genome of various plant species for the production of dsRNA for insect pest control, and for the production of veterinary vaccines

Education and awards

  • PhD. in Molecular Genetics, McGill University
  • M.Sc. in Plant Pathology, American University of Beirut
  • B.Sc. in Agriculture, American University of Beirut

Key publications

  1. Scott, I.M., Zhu, H., Schieck, K., Follick, A., Reynolds, L.B., Menassa, R. (2019). Non-target effects of hyperthermostable α-amylase transgenic Nicotiana tabacum in the laboratory and the field, 10 http://dx.doi.org/10.3389/fpls.2019.00878

    2019 - View publication details

  2. Tran, H.H., Chen, B., Chen, H., Menassa, R., Hao, X., Bernards, M., Hüner, N.P.A., Wang, A. (2019). Development of a cucumber green mottle mosaic virus-based expression vector for the production in cucumber of neutralizing epitopes against a devastating animal virus, 269 18-25. http://dx.doi.org/10.1016/j.jviromet.2019.04.006

    2019 - View publication details

  3. Burke, W.G., Kaplanoglu, E., Kolotilin, I., Menassa, R., Donly, C. (2019). RNA interference in the tobacco hornworm, manduca sexta, using plastid-encoded long double-stranded RNA, 10 http://dx.doi.org/10.3389/fpls.2019.00313

    2019 - View publication details

  4. Donly, C., Kaplanoglu, E., Ludba, K.K., Kolotilin, I., Menassa, R., and Scott, I.M. (2018) Effects of RNA interference on greenhouse whitefly (Trialeurodes vaporariorum). Insect Biotech Conference, St. Catharines, Ontario.

    2018 - View publication details

  5. Feeney, M., Kittelmann, M., Menassa, R., Hawes, C., Frigerio, L. (2018). Protein storage vacuoles originate from remodeled preexisting vacuoles in arabidopsis thaliana, 241-254. http://dx.doi.org/10.1104/pp.18.00010

    2018 - View publication details

  6. Ceballo, Y., Tiel, K., López, A., Cabrera, G., Pérez, M., Ramos, O., Rosabal, Y., Montero, C., Menassa, R., Depicker, A., Hernández, A. (2017). High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza, 26(6), 775-789. http://dx.doi.org/10.1007/s11248-017-0047-9

    2017 - View publication details

  7. Miletic, S., Hünerberg, M., Kaldis, A., MacDonald, J., Leuthreau, A., McAllister, T., Menassa, R. (2017). A Plant-Produced Candidate Subunit Vaccine Reduces Shedding of Enterohemorrhagic Escherichia coli in Ruminants, 12(10), http://dx.doi.org/10.1002/biot.201700405

    2017 - View publication details

  8. Reuter, L.J., Shahbazi, M.A., Mäkilä, E.M., Salonen, J.J., Saberianfar, R., Menassa, R., Santos, H.A., Joensuu, J.J., Ritala, A. (2017). Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells, 28(6), 1639-1648. http://dx.doi.org/10.1021/acs.bioconjchem.7b00075

    2017 - View publication details

  9. Donly, C., Kaplanoglu, E., Ludba, K., Kolotilin, I., Menassa, R., Scott, I. (2017) Insecticidal trap plants incorporating RNA interference technology. Insect Biotech Conference, St. Catharines, Ontario. 14-16 June 2017, oral presentation.

    2017 - View publication details

  10. Saberianfar, R. and Menassa, R. (2017) Protein bodies: how the ER deals with high accumulation of recombinant proteins. Plant Biotechnol J 15, 671-673.

    2017 - View publication details

  11. MacDonald, J., Miletic, S., Gaildry, T., Chin-Fatt, A., Menassa, R. (2017). Co-expression with the type 3 secretion chaperone CesT from enterohemorrhagic E. coli increases accumulation of recombinant Tir in plant chloroplasts, 8 http://dx.doi.org/10.3389/fpls.2017.00283

    2017 - View publication details

  12. Arthur, L.L., Chung, J.J., Jankirama, P., Keefer, K.M., Kolotilin, I., Pavlovic-Djuranovic, S., Chalker, D.L., Grbic, V., Green, R., Menassa, R., True, H.L., Skeath, J.B., Djuranovic, S. (2017). Rapid generation of hypomorphic mutations, 8 http://dx.doi.org/10.1038/ncomms14112

    2017 - View publication details

  13. Zhu, H., Reynolds, L.B., Menassa, R. (2017). A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates, 17(1), http://dx.doi.org/10.1186/s12896-017-0372-3

    2017 - View publication details

  14. Topp, E., Irwin, R., McAllister, T.A., Lessard, M., Joensuu, J.J., Kolotilin, I., Conrad, U., Stöger, E., Mor, T., Warzecha, H., Hall, J.C., McLean, M.D, Cox, E., Devriendt, B., Potter, A., Depicker, A., Virdi, V., Holbrook, L., Doshi, K., Dussault, M., Friendship, R.M., Yarosh, O., Yoo, H.S., MacDonald, J.D., and Menassa, R. (2016). "The case for plant-made veterinary immunotherapeutics.", Biotechnology Advances. doi : 10.1016/j.biotechadv.2016.02.007

    2016 - View publication details

  15. Miletic, S., Simpson, D.J., Szymanski, C.M., Deyholos, M.K., Menassa, R. (2016). A plant-produced bacteriophage tailspike protein for the control of Salmonella, 6(JAN2016), http://dx.doi.org/10.3389/fpls.2015.01221

    2016 - View publication details

  16. Menassa, R. (2015). "Protein body biogenesis and utility in recombinant protein production in Nicotiana benthamiana.", Recombinant Protein Production 8, Palma, Mallorca, Spain, April 22-24, 2015.

    2015 - View publication details

  17. Kolotilin, I., Topp, E., Cox, E., Devriendt, B., Conrad, U., Joensuu, J.J., Stöger, E., Warzecha, H., McAllister, T.A., McLean, M.D, Hall, J.C., and Menassa, R. (2015). "Plant-based solutions for veterinary immunotherapeutics and prophylactics.", BMC Veterinary Research, 45(1), pp. 375. (Abstract)

    2015 - View publication details

  18. Menassa, R. (2015). "Producing veterinary vaccines for bacterial diseases in transplastomic plants.", 2015 Chloroplast Biotechnology Gordon Research Conference, Ventura Beach, California, USA.

    2015 - View publication details

  19. Menassa, R. (2015). "Production and testing of plant-made subunit vaccine candidates for porcine epidemic diarrhea and enterotoxigenic E. coli.", Plant-Based Vaccines, Antibodies and Biologicals 2015, Lausanne, June 8-10, 2015.

    2015 - View publication details

  20. Sattarzadeh, A., Saberianfar, R., Zipfel, W.R., Menassa, R., Hanson, M.R. (2015). Green to red photoconversion of GFP for protein tracking in vivo, 5 http://dx.doi.org/10.1038/srep11771

    2015 - View publication details

Research facility

1391 Sandford Street
London, ON N5V 4T3
Canada

Affiliations

Member of the International Society for Plant Molecular Farming

President of the Canadian Association for Plant Biotechnology

Secretary for Plant Canada

Adjunct professor, Department of Biology, Western University, London, Ontario

Associate editor, Frontiers in Plant Science

Language

English
French