Pankaj Bhowmik, Ph.D
Research Scientist, Cell Technologies and Trait Development
Research Officer, Strategic Research Initiatives
Adjunct Professor, Irving K. Barber School, UBC Okanagan
Current research and/or projects
- Genetic enhancement of protein, off-flavour, anti-nutritional quality and processability in pulse crops.
- An efficient plant protoplasts isolation and transient transformation and regeneration system for CRISPR/Cas9 mediated gene editing
Research and/or project statements
- Protein quality in pea and other pulses is limited by the low concentration of the sulfur amino acids methionine, and cysteine.
- Therefore, enhancement of protein quality by increasing the levels of sulfur amino acids is a priority for new and expanded markets for pea and other pulses.
- Another barrier to increased use of pulse products in the food industry is the off-flavour.
- We aim to manipulate genes of interest (LOX and Vicilin) using the innovative new breeding technique – gene editing for improving both essential amino acids and reducing off-flavour in pea.
Professional activities / interests
- Plant Tissue culture, transformation, gene editing
- Application of cell technologies - protoplasts and microspore-based genetic manipulation for crop improvement
Education and awards
Ehime University - Matsuyama, Ehime | Ph.D.
Kagawa University - Takamatsu, Kagawa | Master of Science
Bangladesh Agricultural University - Mymensingh, Bangladesh | Bachelor of Science
NSERC visiting Fellow in Canadian Government Laboratory: Department of Bioproduct and Bioprocess, Lethbridge Research Centre Agriculture and Agri-Food Canada; September, 2007 – August, 2009
Izaak Walton Killam Postdoctoral Fellowship: Department of AFNS, University of Alberta, Canada; January, 2006 – August, 2007
Japanese Society for the Promotion of Science (JSPS) Postdoctoral Fellowship: April, 2004 – December, 2005
International experience and/or work
- Japan Society for the Promotion of Science (JSPS) Fellow –National Agriculture Research Organization, Sapporo, Japan
- Bangladesh Agriculture Research Institute (BARI) – Scientific Officer
(Refereed Journal = 25 Refereed Book Chapter = 12; Conference Presentation = 15)
Peer reviewed journal
25. Bhowmik, P., Ellison, E., Polley, B., Bollina, V., Kulkarni, M., Ghanbarnia, K., et al. (2018). Targeted mutagenesis in wheat microspores using CRISPR/Cas9. Sci. Rep. 8:6502. doi: 10.1038/s41598-018-24690-8.
24. Rajagopalan, N., Kagale, S., Bhowmik, P., Song, H. (2018). A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies." Methods Protoc. 1, no. 2: 17.
23. Haque, E., Taniguchi, H., Hassan, MM., Bhowmik, P., Karim, MR., Śmiech, M., Zhao, K., Rahman, M. and Islam,T. (2018) Application of CRISPR/Cas9 Genome Editing Technology for the Improvement of Crops Cultivated in Tropical Climates: Recent Progress, Prospects, and Challenges. Front. Plant Sci. 9:617. doi: 10.3389/fpls.2018.00617.
22. Bhowmik P.K, Dirpaul J, Polowick P and Ferrie A. 2011. An efficient high throughput Brassica napus microspore culture system: Influence of percoll gradient separation and precise bud selection on embryogenesis. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-010-9913-3.
21. Chandra, D., Matsui T., Suzuki, H. Kosugi, Y., Fujimura, K., and Bhowmik P.K. 2010. Textural and compositional changes of stored iceberg lettuce in relation to harvest season and storage. International Journal of Vegetable Science 16: 44-59.
20. Aremu, M.O., Basu, S.K., Gyar, S.D., Goyal, A., Bhowmik, P.K. and Datta Banik, S. 2009. Proximate composition and functional properties of mushroom flours from Ganoderma spp., Omphalotus olearius (DC.) Sing. and Hebeloma mesophaeum (Pers.) Quél. used in Nasarawa State, Nigeria. Mal J Nutr 15(2): 233 – 241.
19. Bhowmik, P.K. and Basu S. 2008. Current developments, progress, issues and concerns in producing transgenic peas (Pisum sativum L.). Transgenic Plant Journal 2(2): 138-150.
18. Bhowmik P. K., K. Tamura, Y. Sanada, K. Tase and T. Yamada. 2007. Molecular cloning and characterization of a cDNA encoding sucrose synthase from perennial ryegrass (Lolium perenne L.). Indian Journal of Botanical Research 3(2): 237-250.
17. Bhowmik, P. K. and T. Matsui. 2007. Changes in acid invertase and sucrose synthase activity and gene expression during storage of asparagus spears. Indian Journal of Botanical Research 3(2): 251-264.
16. Bhowmik P. K., K.Tamura, Y. Sanada, K. Tase and T. Yamada. 2006. Sucrose Metabolism of Perennial Ryegrass in Relation to Cold Acclimation. Zeitschrift für Naturforschung C- A Journal of Biosciences 61(c): 99-104.
15. Bhowmik, P. K. and T. Matsui. 2005. Ethylene biosynthetic genes in ‘Moso' bamboo shoot in response to wounding. Postharvest Biology and Technology 38 (2): 188-194.
14. Bhowmik, P. K. and T. Matsui. 2005. Changes in phenylalanine ammonia lyase activity and gene expression during storage of asparagus spears. Zeitschrift für Naturforschung C- A Journal of Biosciences. 60 (2): 128-132.
13. Bhowmik, P. K. and T. Matsui. 2004. Changes in the Activity and Expression of 1 Aminocyclopropane -1-Carboxylate (ACC) Synthase, ACC Oxidase, and Phenylalanine Ammonia-Lyase in Asparagus Spears in Response to Wound- Induced Ethylene Synthesis. HortScience 39(6):1074-1078.
12. Bhowmik, P.K., T. Matsui and E. Miki. 2004. α-aminooxi-β-phenylpropionic acid controls phenylalanine ammonia lyase gene expression during storage of asparagus spears. Journal of Food, Agriculture & Environment 2(1): 10-13.
11. Bhowmik, P. K., T. Matsui, F. G. Enriquez, A. K. M. S. Alam, H. Suzuki and Y. Kosugi. 2003. Pre- and postharvest fiber development in green asparagus as related to temperature during growth, storage, and handling. Journal of Food, Agriculture Environment 1(4): 50-53.
10. Bhowmik, P. K., T. Matsui, T. Ikeuchi and H. Suzuki. 2002. Changes in storage quality and shelf life of green asparagus over an extended harvest season. Postharvest Biology and Technology. 26(3): 323-328.
9. Bhowmik, P.K., T. Matsui, K. Kawada, and H. Suzuki. 2001. Seasonal changes of asparagus spears in relation to enzyme activities and carbohydrate content. Scientia Horticulturae. 88(1): 1-9.
8. Bhowmik, P. K. T. Matsui and A. K. M. S. Alam. 2004.Cloning and sequencing of an ACC Synthase cDNA from asparagus spears and its expression during storage. Asian Journal of Plant Sciences. 3(1): 25-30.
7. Bhowmik, P. K., T. Matsui, M. Fujita, M. Z. Hossain and E. Miki 2004. Phenylalanine ammonia lyase inhibitors-2,5-Norbornadiene and α-Aminooxi-β-Phenylpropionic acid reduce lignification and toughening of stored asparagus spears. Asian Journal of Plant Sciences.3(1): 50-54.
6. Bhowmik, P. K. and T. Matsui. 2003. Carbohydrate status and sucrose metabolism in asparagus roots over an extended harvest season. Asian Journal of Plant Sciences. 2(12): 891-893.
5. Bhowmik, P. K. and T. Matsui. 2003. Postharvest physiology, storage and keeping quality of green asparagus: a review. Asian Journal of Plant Sciences. 2(12): 941-943.
4. Bhowmik, P. K., T. Matsui, H. Suzuki and Y. Kosugi. 2003. A phenylalanine ammonia-lyase gene from asparagus: cDNA cloning, sequence and expression in response to wounding. Asian Journal of Plant Sciences. 2(5): 425-430.
3. Bhowmik, P. K., T. Matsui, H. Suzuki and Y. Kosugi. 2003. Glutamine synthetase in harvested asparagus spears: Changes in activity and gene expression during storage. Asian Journal of Plant Sciences. 2(1):102-107.
2. Bhowmik, P. K., T. Matsui, H. Suzuki and Y. Kosugi. 2002. A harvest-induced ACC Oxidase gene from tips of harvested asparagus spears and its expression during storage. Asian Journal of Plant Sciences. 1(4): 390-394.
1. Bhowmik, P.K., T. Matsui, and Z. Ahmed. 2001. Changes in the activity of sucrose metabolizing enzymes during the germination of wheat seeds. Pak.J.Biol.Sci. 4(10): 1264- 1266.
Executive member of the Canadian Association for Plant Biotechnology (CAPB) Web site: http://www.canadianplantbiotech.ca/; January, 2014 – Present